Lymphocyte-specific protein 1 expression in eukaryotic cells reproduces the morphologic and motile abnormality of NAD 47/89 neutrophils.
AuthorsHoward TH, Hartwig J, Cunningham C
JournalBlood
PubMed ID9616178
'Despite its name, the actin-binding protein lymphocyte-specific protein1 (LSP1) is found in all hematopoetic cells, and yet its role in cell function remains unclear. Recently, LSP1 was identified as the 47- kD protein overexpressed in the polymorphonuclear neutrophils of patients with a rare neutrophil disorder, neutrophil actin dysfunction with abnormalities ... More
Cloning and analysis of the murine Fanconi anemia group C cDNA.
AuthorsWevrick R, Clarke CA, Buchwald M
JournalHum Mol Genet
PubMed ID7689006
'Fanconi anemia (FA) is one of a group of disorders characterized at the cellular level by a combination of hypersensitivity to DNA-damaging agents, chromosomal instability, and defective DNA repair. Clinical features of FA include pancytopenia, often accompanied by specific congenital malformations, and a predisposition to leukemia. Since the hematological manifestations ... More
Protein kinase C isotypes in human erythroleukemia (K562) cell proliferation and differentiation. Evidence that beta II protein kinase C is required for proliferation.
AuthorsMurray NR, Baumgardner GP, Burns DJ, Fields AP
JournalJ Biol Chem
PubMed ID8340409
'The human erythroleukemia (K562) cell line undergoes megakaryocytic differentiation and cessation of proliferation when treated with phorbol myristate acetate (PMA). To investigate the role of individual protein kinase C (PKC) isotypes in these events, we have assessed PKC isotype expression during leukemic proliferation and PMA-induced differentiation. Immunoblot analysis using isotype-specific ... More
A requirement for extracellular signal-regulated kinase (ERK) function in the activation of AP-1 by Ha-Ras, phorbol 12-myristate 13-acetate, and serum.
AuthorsFrost JA, Geppert TD, Cobb MH, Feramisco JR
JournalProc Natl Acad Sci U S A
PubMed ID8170999
'The role of ERK-1 and ERK-2 in wild-type (wt) Ha-Ras, phorbol 12- myristate 13-acetate (PMA), and serum-induced AP-1 activity was studied. Microinjection of ERK-specific substrate peptides inhibited the induction of AP-1 activity by all three stimuli, whereas a control peptide had no effect. By using eukaryotic expression constructs encoding wt ... More
Stat5 is a physiological substrate of the insulin receptor.
AuthorsChen J, Sadowski HB, Kohanski RA, Wang LH
JournalProc Natl Acad Sci U S A
PubMed ID9122188
'Using the cytoplasmic domain of the insulin receptor (IR) in a yeast two-hybrid screen, we identified a cDNA clone encoding the C-terminal 308 amino acids of human Stat5b (Stat5b-Ct). Stat5b-Ct is tyrosine phosphorylated by purified IR kinase domain in vitro. Insulin stimulates tyrosine phosphorylation of overexpressed Stat5b-Ct and endogenous Stat5 ... More
Discoordinate expression of invariant chain and MHC class II genes in class II transactivator-transfected fibroblasts defective for RFX5 [In Process Citation]
AuthorsPeijnenburg A, Van Eggermond MJ, Gobin SJ, Van den Berg R, Godthelp BC, Vossen JM, Van den Elsen PJ
JournalJ Immunol
PubMed ID10395672
'MHC class II deficiency or bare lymphocyte syndrome is a severe combined immunodeficiency caused by defects in MHC-specific transcription factors. In the present study, we show that fibroblasts derived from a recently identified bare lymphocyte syndrome patient, SSI, were mutated for RFX5, one of the DNA-binding components of the RFX ... More
The RFX complex is crucial for the constitutive and CIITA-mediated transactivation of MHC class I and beta2-microglobulin genes.
AuthorsGobin SJ, Peijnenburg A, van Eggermond M, van Zutphen M, van den Berg R, van den Elsen PJ
JournalImmunity
PubMed ID9806639
In type III bare lymphocyte syndrome (BLS) patients, defects in the RFX protein complex result in a lack of MHC class II and reduced MHC class I cell surface expression. Using type III BLS cell lines, we demonstrate that the RFX subunits RFX5 and RFXAP are crucial for constitutive and ... More
RFX-B is the gene responsible for the most common cause of the bare lymphocyte syndrome, an MHC class II immunodeficiency.
AuthorsNagarajan UM, Louis-Plence P, DeSandro A, Nilsen R, Bushey A, Boss JM
JournalImmunity
PubMed ID10072068
The bare lymphocyte syndrome (BLS) is characterized by the absence of MHC class II transcription and humoral- and cellular-mediated immune responses to foreign antigens. Three of the four BLS genetic complementation groups have defects in the activity of the MHC class II transcription factor RFX. We have purified the RFX ... More
LINEs mobilize SINEs in the eel through a shared 3' sequence.
AuthorsKajikawa Masaki; Okada Norihiro;
JournalCell
PubMed ID12453401
We characterized members of the LINE (UnaL2) and SINE (UnaSINE1) families from the eel genome and found that these LINE/SINE partners share similar 3' tails. A retrotransposition assay in HeLa cells demonstrated that the 3' conserved tail of UnaL2 is necessary for its retrotransposition. This 3' tail is recognized in ... More
Site alpha is crucial for two routes of IFN gamma-induced MHC class I transactivation: the ISRE-mediated route and a novel pathway involving CIITA.
AuthorsGobin SJ, Peijnenburg A, Keijsers V, van den Elsen PJ
JournalImmunity
PubMed ID9175838
The constitutive and cytokine-induced levels of major histocompatibility (MHC) class I expression are tightly controlled at the transcriptional level. In this study, it is shown that the cis- acting regulatory element site alpha of the MHC class I promoter is essential for the IFN gamma-induced transactivation of MHC class I ... More
The human DNA ligase III gene encodes nuclear and mitochondrial proteins.
AuthorsLakshmipathy U, Campbell C
JournalMol Cell Biol
PubMed ID10207110
We provide evidence that the human DNA ligase III gene encodes a mitochondrial form of this enzyme. First, the DNA ligase III cDNA contains an in-frame ATG located upstream from the putative translation initiation start site. The DNA sequence between these two ATG sites encodes an amphipathic helix similar to ... More
Pseudotyping of murine leukemia virus with the envelope glycoproteins of HIV generates a retroviral vector with specificity of infection for CD4-expressing cells.
AuthorsSchnierle BS, Stitz J, Bosch V, Nocken F, Merget-Millitzer H, Engelstadter M, Kurth R, Groner B, Cichutek K
JournalProc Natl Acad Sci U S A
PubMed ID9238030
CD4-expressing T cells in lymphoid organs are infected by the primary strains of HIV and represent one of the main sources of virus replication. Gene therapy strategies are being developed that allow the transfer of exogenous genes into CD4(+) T lymphocytes whose expression might prevent viral infection or replication. Insights ... More
Beta-adrenergic receptor kinase (GRK2) colocalizes with beta-adrenergic receptors during agonist-induced receptor internalization.
AuthorsRuiz-Gomez A, Mayor F Jr
JournalJ Biol Chem
PubMed ID9092483
Rapid regulation of G protein-coupled receptors appears to involve agonist-promoted receptor phosphorylation by G protein-coupled receptor kinases (GRKs). This is followed by binding of uncoupling proteins termed arrestins and transient receptor internalization. In this report we show that the beta-adrenergic receptor kinase (betaARK-1 or GRK2) follows a similar pattern of ... More
The role of the C-terminal domain of I kappa B alpha in protein degradation and stabilization.
AuthorsBeauparlant P, Lin R, Hiscott J
JournalJ Biol Chem
PubMed ID8631876
In the present study, the role of the I kappa B alpha C terminus in NF- kappa B/I kappa B alpha regulation was examined in NIH 3T3 cells engineered to inducibly express wild type or mutated human I kappa B alpha proteins under the control of the tetracycline responsive promoter. ... More
In vitro comparison of the antigen-binding and stability properties of the various molecular forms of IgA antibodies assembled and produced in CHO cells.
AuthorsBerdoz J, Blanc CT, Reinhardt M, Kraehenbuhl JP, Corthesy B
JournalProc Natl Acad Sci U S A
PubMed ID10077631
The hallmark of a mucosal immune response is the production of antigen- specific secretory IgA (S-IgA) antibodies in external secretions. S-IgA consists of ten polypeptides produced in two different cell lineages. The heavy and light chains in plasma cells assemble into IgA, which on association with J chain become polymerized, ... More
Enhanced responses to a DNA vaccine encoding a fusion antigen that is directed to sites of immune induction.
AuthorsBoyle JS, Brady JL, Lew AM
JournalNature
PubMed ID9537327
Viral infection and vaccination with DNA both induce similar immune responses to encoded antigens that are produced by the host. The availability of antigens in lymphoid organs is important in generating an immune response to viral challenge. Antigen availability may also be important in the response to DNA vaccines, because ... More
Transfection and overexpression of the calcium binding protein calbindin-D28k results in a stimulatory effect on insulin synthesis in a rat beta cell line (RIN 1046-38).
AuthorsReddy D, Pollock AS, Clark SA, Sooy K, Vasavada RC, Stewart AF, Honeyman T, Christakos S
JournalProc Natl Acad Sci U S A
PubMed ID9050887
Calbindin-D28k, a calcium binding protein that is thought to act as a facilitator of calcium diffusion in intestine and kidney, is known to be regulated by vitamin D in these tissues. Calbindin-D28k is also present in pancreatic beta cells, but its function in these cells is not known. To determine ... More
Identification of candidate active site residues in lysosomal beta- hexosaminidase A.
AuthorsFernandes MJ, Yew S, Leclerc D, Henrissat B, Vorgias CE, Gravel RA, Hechtman P, Kaplan F
JournalJ Biol Chem
PubMed ID8995368
The beta-hexosaminidases (Hex) catalyze the cleavage of terminal amino sugars on a broad spectrum of glycoconjugates. The major Hex isozymes in humans, Hex A, a heterodimer of alpha and beta subunits (alphabeta), and Hex B, a homodimer of beta subunits (betabeta), have different substrate specificities. The beta subunit (HEXB gene ... More
Epstein-Barr virus shuttle vector for stable episomal replication of cDNA expression libraries in human cells.
AuthorsMargolskee RF, Kavathas P, Berg P,
JournalMol Cell Biol
PubMed ID2841588
Efficient transfection and expression of cDNA libraries in human cells has been achieved with an Epstein-Barr virus-based subcloning vector (EBO-pcD). The plasmid vector contains a resistance marker for hygromycin B to permit selection for transformed cells. The Epstein-Barr virus origin for plasmid replication (oriP) and the Epstein-Barr virus nuclear antigen ... More
Comparison of filamin A-induced cross-linking and Arp2/3 complex-mediated branching on the mechanics of actin filaments.
Authors Nakamura Fumihiko; Osborn Eric; Janmey Paul A; Stossel Thomas P;
JournalJ Biol Chem
PubMed ID11786548
We compared the effects of human filamin A (FLNa) and the activated human Arp2/3 complex on mechanical properties of actin filaments. As little as 1 FLNa to 800 polymerizing actin monomers induces a sharp concentration-dependent increase in the apparent viscosity of 24 microm actin, a parameter classically defined as a ... More