Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype.
AuthorsRobertson FM, Ogasawara MA, Ye Z, Chu K, Pickei R, Debeb BG, Woodward WA, Hittelman WN, Cristofanilli M, Barsky SH,
JournalJ Biomol Screen
PubMed ID20639504
'Tumors that display a highly metastatic phenotype contain subpopulations of cells that display characteristics similar to embryonic stem cells. These cells exhibit the ability to undergo self-renewal; slowly replicate to retain a nucleoside analog label, leading to their definition as ' ... More
Classic
AuthorsHenderson L, Bortone DS, Lim C, Zambon AC,
JournalAm J Physiol Cell Physiol
PubMed ID23392113
'Many common, important diseases are either caused or exacerbated by hyperactivation (e.g., cancer) or inactivation (e.g., heart failure) of the cell division cycle. A better understanding of the cell cycle is critical for interpreting numerous types of physiological changes in cells. Moreover, new insights into how to control it will ... More
Differential effects of cocaine on histone posttranslational modifications in identified populations of striatal neurons.
AuthorsJordi E, Heiman M, Marion-Poll L, Guermonprez P, Cheng SK, Nairn AC, Greengard P, Girault JA,
Journal
PubMed ID23690581
'Drugs of abuse, such as cocaine, induce changes in gene expression and epigenetic marks including alterations in histone posttranslational modifications in striatal neurons. These changes are thought to participate in physiological memory mechanisms and to be critical for long-term behavioral alterations. However, the striatum is composed of multiple cell types, ... More
Sponge hybridomas: applications and implications.
AuthorsPomponi SA, Jevitt A, Patel J, Diaz MC,
JournalIntegr Comp Biol
PubMed ID23639719
'Many sponge-derived natural products with applications to human health have been discovered over the past three decades. In vitro production has been proposed as one biological alternative to ensure adequate supply of marine natural products for preclinical and clinical development of drugs. Although primary cell cultures have been established for ... More
Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus.
AuthorsYamamoto Y, Watanabe T, Hoki Y, Shirane K, Li Y, Ichiiyanagi K, Kuramochi-Miyagawa S, Toyoda A, Fujiyama A, Oginuma M, Suzuki H, Sado T, Nakano T, Sasaki H,
JournalGenome Res
PubMed ID23132912
'In germ cells, early embryos, and stem cells of animals, PIWI-interacting RNAs (piRNAs) have an important role in silencing retrotransposons, which are vicious genomic parasites, through transcriptional and post-transcriptional mechanisms. To examine whether the piRNA pathway can be used to silence genes of interest in germ cells, we have generated ... More
Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis.
AuthorsKoltowska K, Apitz H, Stamataki D, Hirst EM, Verkade H, Salecker I, Ober EA,
JournalDevelopment
PubMed ID23515471
Tightly controlled DNA replication and RNA transcription are essential for differentiation and tissue growth in multicellular organisms. Histone chaperones, including the FACT (facilitates chromatin transcription) complex, are central for these processes and act by mediating DNA access through nucleosome reorganisation. However, their roles in vertebrate organogenesis are poorly understood. Here, ... More
Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development.
AuthorsIchiyanagi K, Li Y, Li Y, Watanabe T, Ichiyanagi T, Fukuda K, Kitayama J, Yamamoto Y, Kuramochi-Miyagawa S, Nakano T, Yabuta Y, Seki Y, Saitou M, Sasaki H,
JournalGenome Res
PubMed ID22042642
In mammals, germ cells undergo striking dynamic changes in DNA methylation during their development. However, the dynamics and mode of methylation are poorly understood for short interspersed elements (SINEs) dispersed throughout the genome. We investigated the DNA methylation status of mouse B1 SINEs in male germ cells at different developmental ... More
A method for evaluating the use of fluorescent dyes to track proliferation in cell lines by dye dilution.
AuthorsBegum J, Day W, Henderson C, Purewal S, Cerviera J, Summers H, Rees P, Davies D, Filby A,
Journal
PubMed ID24166880
Labeling nonquiescent cells with carboxyfluorescein succinimidyl ester (CFSE)-like dyes gives rise to a population width exceeding the threshold for resolving division peaks by flow cytometry. Width is a function of biological heterogeneity plus extrinsic and intrinsic error sources associated with the measurement process. Optimal cytometer performance minimizes extrinsic error, but ... More