含有 Annexin V Alexa Fluor 488 和 PI 的死细胞凋亡试剂盒 含有 Annexin V Alexa Fluor 488 和碘化丙啶 (PI) 的死细胞凋亡试剂盒 (V13241, V13245) 用于进行流式细胞分析,通过检测磷脂酰丝氨酸 (PS) 表达和膜通透性来测量早期细胞凋亡。当细胞用 Annexin V 和碘化丙啶染色时,表达 PS 的凋亡细胞显示绿色荧光,可在 FITC 通道中检测到,并显示低红色荧光。死亡或坏死细胞显示明亮的红色荧光,无绿色荧光,而活细胞显示无绿色或红色荧光。
含有 Annexin V PE 和 SYTOX Green 的死细胞凋亡试剂盒 含有 Annexin V PE 和 SYTOX Green 的死细胞凋亡试剂盒可在流式细胞分析期间使用偶联橙色荧光藻胆蛋白 R-PE 的重组膜联蛋白 V 检测凋亡细胞的 PS 外化,死细胞则用 SYTOX Green 核酸染色剂进行检测。经过两种探针处理后,凋亡细胞显示橙色荧光,死细胞显示绿色荧光,活细胞几乎不显示荧光。在使用 488 nm 激光流式细胞仪的 530/30 nm 和 585/42 nm 带通滤光片中可轻松区分这些细胞群。
含有 Annexin V APC 和 SYTOX Green 的死细胞凋亡试剂盒 含有 Annexin V APC 和 SYTOX Green 的死细胞凋亡试剂盒可在流式细胞分析期间使用偶联红色激光激发别藻蓝蛋白的重组膜联蛋白 V 检测凋亡细胞的 PS 外化,死细胞则用 SYTOX Green 核酸染色剂进行检测。通过与外化 PS 结合的膜联蛋白 V 检测凋亡细胞。活细胞几乎不显示荧光,凋亡细胞显示红色荧光和极少的绿色荧光,晚期凋亡细胞显示较高水平的红色和橙色荧光。这些细胞群可以很容易地通过含有 488 nm 和 633 nm 激发源(氩离子激光器和 HeNe 激光器)的流式细胞仪加以区分。
含 Annexin V FITC 和 PI 的死细胞凋亡试剂盒 含 Annexin V FITC 和 PI 的死细胞凋亡试剂盒可使用偶联绿色荧光 FITC 染料的重组膜联蛋白 V 检测凋亡细胞的 PS 外化,死细胞则用 PI 进行检测。经 PI 染色的坏死细胞显示红色荧光。两种探针处理后,凋亡细胞显示绿色荧光,死细胞显示红色和绿色荧光,活细胞显示少量或无荧光。
I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?
It has been done, but we dont recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.
A novel sulindac derivative inhibits lung adenocarcinoma cell growth through suppression of Akt/mTOR signaling and induction of autophagy.
Authors:Gurpinar E, Grizzle WE, Shacka JJ, Mader BJ, Li N, Piazza NA, Russo S, Keeton AB, Piazza GA
Journal:Mol Cancer Ther
PubMed ID:23443799
'Nonsteroidal anti-inflammatory drugs such as sulindac sulfide have shown promising antineoplastic activity in multiple tumor types, but toxicities resulting from COX inhibition limit their use in cancer therapy. We recently described a N,N-dimethylethyl amine derivative of sulindac sulfide, sulindac sulfide amide (SSA), that does not inhibit COX-1 or -2, yet ... More
Translation of Pur-a is targeted by cellular miRNAs to modulate the differentiation-dependent susceptibility of monocytes to HIV-1 infection.
Authors:Shen CJ, Jia YH, Tian RR, Ding M, Zhang C, Wang JH,
Journal:FASEB J
PubMed ID:22835829
'The postentry restriction of HIV-1 replication in monocytes can be relieved when they differentiate to dendritic cells (DCs) or macrophages. Multiple mechanisms have been proposed to interpret the differentiation-dependent susceptibility of monocytes to HIV-1 infection, and the absence of host-cell-encoded essential factors for HIV-1 completing the life cycle may provide ... More
DNA methyltransferase-3-dependent nonrandom template segregation in differentiating embryonic stem cells.
'Asymmetry of cell fate is one fundamental property of stem cells, in which one daughter cell self-renews, whereas the other differentiates. Evidence of nonrandom template segregation (NRTS) of chromosomes during asymmetric cell divisions in phylogenetically divergent organisms, such as plants, fungi, and mammals, has already been shown. However, before this ... More
Id4 Promotes Senescence and Sensitivity to Doxorubicin-induced Apoptosis in DU145 Prostate Cancer Cells.
Inhibitor of differentiation proteins (Id1, 2, 3 and 4) are dominant negative regulators of basic helix loop helix transcription factors and play dominant roles in cancer cells, spanning several molecular pathways including senescence, invasion, metastasis, proliferation and apoptosis. In contrast to high Id1, Id2 and Id3 expression, the expression of ... More
The HSP90 Inhibitor Ganetespib Radiosensitizes Human Lung Adenocarcinoma Cells.
Authors:Gomez-Casal R, Bhattacharya C, Epperly MW, Basse PH, Wang H, Wang X, Proia DA, Greenberger JS, Socinski MA, Levina V,
Journal:
PubMed ID:26010604
The molecular chaperone HSP90 is involved in stabilization and function of multiple client proteins, many of which represent important oncogenic drivers in NSCLC. Utilization of HSP90 inhibitors as radiosensitizing agents is a promising approach. The antitumor activity of ganetespib, HSP90 inhibitor, was evaluated in human lung adenocarcinoma (AC) cells for ... More