Annexin 结合缓冲液 (5X)（用于流式细胞术）
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Annexin 结合缓冲液 (5X)（用于流式细胞术）
引用和文献 (6)
Invitrogen™

Annexin 结合缓冲液 (5X)（用于流式细胞术）

该产品设计用于促进 Annexin V 与磷脂酰丝氨酸结合，以检测细胞凋亡。了解更多信息
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货号数量
V1324650 mL
货号 V13246
价格（CNY)
802.00
飞享价
Ends: 31-Dec-2025
1,065.00
共减 263.00 (25%)
50 mL
添加至购物车
数量:
50 mL
价格（CNY)
802.00
飞享价
Ends: 31-Dec-2025
1,065.00
共减 263.00 (25%)
50 mL
添加至购物车
该产品设计用于促进 Annexin V 与磷脂酰丝氨酸结合，以检测细胞凋亡。
仅供科研使用。不可用于诊断程序。
规格
适用于（应用）流式细胞分析
形式浓缩型
配方50 mM HEPES，700 mM NaCl，12.5 mM CaCl2，pH 7.4
产品类型膜联蛋白结合缓冲液
数量50 mL
运输条件湿冰
pH7.4
Unit Size50 mL
内容与储存
含有1瓶膜联蛋白结合缓冲液（5x 溶液，50 mL）。

储存在 2–8°C 下。

常见问题解答 (FAQ)

我用胰蛋白酶消化贴壁细胞并用膜联蛋白V标记，现在甚至在对照的未处理细胞中，流式数据结果都显示出高比例的细胞凋亡，这是什么原因所致？

使用胰蛋白酶消化或机械刮擦细胞暂时破坏质膜，使得膜联蛋白V结合到细胞膜胞内面上的磷脂酰丝氨酸，导致假阳性染色。胰蛋白酶消化/机械刮擦后，让细胞在最佳的细胞培养条件和培养基中复苏约30分钟，从而在染色前恢复细胞膜完整性。对于轻微粘连细胞系，例如HeLa和NIH 3T3，还可使用无酶处理方法，例如使用Gibco 细胞溶解缓冲液（货号13151014）。

我能在成像实验中检测膜联蛋白V染色么？

膜联蛋白V染色一般不用于成像实验；而是流式细胞仪分析的最佳试剂。所有的细胞染色程度都会相近，因此很难从暗的非凋亡细胞中分辨出相对明亮的膜联蛋白V染色的细胞。使用我们的CellEvent Caspase 3/7 或Image-iT LIVE Caspase检测试剂盒检测caspase酶活化，这是用于成像实验检测凋亡的最佳方法。

流式细胞仪分析时，我应该在什么时候用膜联蛋白V染色粘连细胞？在胰蛋白酶消化前或后？

先用胰蛋白酶消化，用膜联蛋白V偶联染料染色之前，在合适的细胞培养条件和培养基中复苏约30分钟。用胰蛋白酶消化或机械刮擦细胞暂时打乱了质膜，使得膜联蛋白V连接到细胞膜的胞内面上的磷脂酰丝氨酸，因此导致假阳性染色。对于轻微粘连细胞系例如HeLa和NIH 3T3，您可以使用低强度（无酶）的解离产品如Gibco Cell Dissociation Buffer（货号13151014）。

我能固定膜联蛋白V标记的细胞么？

膜联蛋白V染色分析最好在是活细胞上进行。如果您需要固定您的细胞并用于分析，用3.7%甲醛在含有钙和镁的PBS溶液中固定可以维持连接。透化作用后信号不会保留，因此膜联蛋白V染色无法与内部抗体标记兼容。 

I trypsinized my adherent cells and labeled with annexin V, and now my flow data is showing a high percentage of apoptotic cells even for control, untreated cells. What is the problem?

Trypsinization or mechanical scraping of cells temporarily disrupts the plasma membrane, allowing annexin V to bind phosphatidylserine on the cytoplasmic surface of the cell membrane and thus leading to false positive staining. Allow the cells to recover for about 30 minutes in optimal cell culture conditions and medium after trypsinizing/scraping so that they can recover their membrane integrity before staining. For lightly adherent cell lines, such as HeLa and NIH 3T3, another option is to use non-enzyme treatments like Gibco Cell Dissociation Buffer (Cat. No. 13151014).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Annexin 结合缓冲液 (5X)（用于流式细胞术） FAQs

引用和文献 (6)

引用和文献
Abstract
Surface functionalization of doxorubicin-loaded liposomes with octa-arginine for enhanced anticancer activity.
Authors:Biswas S, Dodwadkar NS, Deshpande PP, Parab S, Torchilin VP
Journal:Eur J Pharm Biopharm
PubMed ID:23333899
Doxorubicin-loaded PEGylated liposomes (commercially available as DOXIL or Lipodox) were surface functionalized with a cell-penetrating peptide, octa-arginine (R8). For this purpose, R8-peptide was conjugated to the polyethylene glycol-dioleoyl phosphatidylethanolamine (PEG-DOPE) amphiphilic co-polymer. The resultant R8-PEG-PE conjugate was introduced into the lipid bilayer of liposomes at 2 mol% of total lipid ... More
Bruton's tyrosine kinase is at the crossroads of metabolic adaptation in primary malignant human lymphocytes.
Authors:Sharif-Askari B, Doyon D, Paliouras M, Aloyz R
Journal:Sci Rep
PubMed ID:31363127
'In this work we explored metabolic aspects of human primary leukemic lymphocytes that hold a potential impact on the treatment of Bruton tyrosine kinase (BTK)-driven diseases. Our results suggest that there is crosstalk between Bruton tyrosine kinase (BTK) signaling and bioenergetic stress responses. In primary chronic lymphocytic leukemia (CLL) lymphocytes, ... More
Ibrutinib Resistance Is Reduced by an Inhibitor of Fatty Acid Oxidation in Primary CLL Lymphocytes.
Authors:Galicia-Vázquez G, Aloyz R
Journal:Front Oncol
PubMed ID:30319974
Chronic Lymphocytic Leukemia (CLL) is an incurable disease, characterized by the accumulation of malignant B-lymphocytes in the blood stream (quiescent state) and homing tissues (where they can proliferate). In CLL, the targeting of B-cell receptor signaling through a Burton's tyrosine kinase inhibitor (ibrutinib) has rendered outstanding clinical results. However, complete ... More
A tyrosine kinase-activating variant Asn666Ser in PDGFRB causes a progeria-like condition in the severe end of Penttinen syndrome.
Authors:Bredrup C, Stokowy T, McGaughran J, Lee S, Sapkota D, Cristea I, Xu L, Tveit KS, Høvding G, Steen VM, Rødahl E, Bruland O, Houge G
Journal:Eur J Hum Genet
PubMed ID:30573803
Missense variants located to the "molecular brake" in the tyrosine kinase hinge region of platelet-derived growth factor receptor-ß, encoded by PFGFRB, can cause Penttinen-type (Val665Ala) and Penttinen-like (Asn666His) premature ageing syndromes, as well as infantile myofibromatosis (Asn666Lys and Pro660Thr). We have found the same de novo PDGFRB c.1997A>G p.(Asn666Ser) variants ... More
Functional Autophagic Flux Regulates AgNP Uptake And The Internalized Nanoparticles Determine Tumor Cell Fate By Temporally Regulating Flux.
Authors:Fageria L, Bambroo V, Mathew A, Mukherjee S, Chowdhury R, Pande S
Journal:Int J Nanomedicine
PubMed ID:31819419
Silver nanoparticles (AgNPs) are known to induce the conserved, cellular, homeostatic process- autophagy in tumor cells. Previous studies primarily focus on the pro-survival role of autophagy post AgNP exposure in tumor cells, but seldom on its role in AgNP uptake, or on the functional significance of autophagy temporal dynamics. Our ... More
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