Rhodopsin kinase: expression in mammalian cells and a two-step purification.
AuthorsBruel C, Cha K, Reeves PJ, Getmanova E, Khorana HG
JournalProc Natl Acad Sci U S A
PubMed ID10737781
'A suitable system for expression of the rhodopsin kinase (RK) gene and its mutants is needed for structure-function studies of RK. Previously, investigation of the baculovirus system showed satisfactory production of RK, but posttranslational isoprenylation was deficient. We now report on a comparative study of expression of the RK gene ... More