Submillisecond optical reporting of membrane potential in situ using a neuronal tracer dye.
AuthorsBradley J, Luo R, Otis TS, DiGregorio DA,
JournalJ Neurosci
PubMed ID19625510
'A major goal in neuroscience is the development of optical reporters of membrane potential that are easy to use, have limited phototoxicity, and achieve the speed and sensitivity necessary for detection of individual action potentials in single neurons. Here we present a novel, two-component optical approach that attains these goals. ... More
Nanotubular highways for intercellular organelle transport.
AuthorsRustom A, Saffrich R, Markovic I, Walther P, Gerdes HH
JournalScience
PubMed ID14963329
Cell-to-cell communication is a crucial prerequisite for the development and maintenance of multicellular organisms. To date, diverse mechanisms of intercellular exchange of information have been documented, including chemical synapses, gap junctions, and plasmodesmata. Here, we describe highly sensitive nanotubular structures formed de novo between cells that create complex networks. These ... More
Sulfated glycosphingolipid as mediator of phagocytosis: SM4s enhances apoptotic cell clearance and modulates macrophage activity.
AuthorsPopovic ZV, Sandhoff R, Sijmonsma TP, Kaden S, Jennemann R, Kiss E, Tone E, Autschbach F, Platt N, Malle E, Gröne HJ,
JournalJ Immunol
PubMed ID17982067
Sulfoglycolipids are present on the surface of a variety of cells. The sulfatide SM4s is increased in lung, renal, and colon cancer and is associated with an adverse prognosis, possibly due to a low immunoreactivity of the tumor. As macrophages significantly contribute to the inflammatory infiltrate in malignancies, we postulated ... More
Surfactant protein-A and toll-like receptor-4 modulate immune functions of preterm baboon lung dendritic cell precursor cells.
AuthorsAwasthi S, Madhusoodhanan R, Wolf R,
JournalCell Immunol
PubMed ID21439559
Lung infections are important risk factors for an increased morbidity and mortality in prematurely-delivered babies. Immaturity of the innate immune components makes them extremely susceptible to infection. Recently, we isolated lung dendritic cell (DC)-precursor cells from preterm fetal baboons. The isolated cells were found to be defective in phagocytosing Escherichia ... More
Fluorescent dyes for lymphocyte migration and proliferation studies.
AuthorsParish CR
JournalImmunol Cell Biol
PubMed ID10571670
Fluorescent dyes are increasingly being exploited to track lymphocyte migration and proliferation. The present paper reviews the properties and performance of some 14 different fluorescent dyes that have been used during the last 20 years to monitor lymphocyte migration. Of the 14 dyes discussed, two stand out as being the ... More
Galectin-3 interaction with Thomsen-Friedenreich disaccharide on cancer-associated MUC1 causes increased cancer cell endothelial adhesion.
AuthorsYu LG, Andrews N, Zhao Q, McKean D, Williams JF, Connor LJ, Gerasimenko OV, Hilkens J, Hirabayashi J, Kasai K, Rhodes JM
JournalJ Biol Chem
PubMed ID17090543
Patients with metastatic cancer commonly have increased serum galectin-3 concentrations, but it is not known whether this has any functional implications for cancer progression. We report that MUC1, a large transmembrane mucin protein that is overexpressed and aberrantly glycosylated in epithelial cancer, is a natural ligand for galectin-3. Recombinant galectin-3 ... More
Activation of Skeletal Stem and Progenitor Cells for Bone Regeneration Is Driven by PDGFRß Signaling.
AuthorsBöhm AM, Dirckx N, Tower RJ, Peredo N, Vanuytven S, Theunis K, Nefyodova E, Cardoen R, Lindner V, Voet T, Van Hul M, Maes C
JournalDev Cell
PubMed ID31543445
'Bone repair and regeneration critically depend on the activation and recruitment of osteogenesis-competent skeletal stem and progenitor cells (SSPCs). Yet, the origin and triggering cues for SSPC propagation and migration remain largely elusive. Through bulk and single-cell transcriptome profiling of fetal osterix (Osx)-expressing cells, followed by lineage mapping, cell tracing, ... More
Left Habenula Mediates Light-Preference Behavior in Zebrafish via an Asymmetrical Visual Pathway.
Authors
JournalNeuron
PubMed ID28190643
Transcription factor TBX4 regulates myofibroblast accumulation and lung fibrosis.
Authors
JournalJ Clin Invest
PubMed ID27400124
Post-surgical adhesions are triggered by calcium-dependent membrane bridges between mesothelial surfaces.
Authors
JournalNat Commun
PubMed ID32555155
Mesenchymal stem cell-derived extracellular vesicles ameliorate Alzheimer's disease-like phenotypes in a preclinical mouse model.
Authors
JournalTheranostics
PubMed ID34373732
CD4+ T-cell-Mediated Rejection of MHC Class II-Positive Tumor Cells Is Dependent on Antigen Secretion and Indirect Presentation on Host APCs.
Authors
JournalCancer Res
PubMed ID29752262
Visual intracortical and transthalamic pathways carry distinct information to cortical areas.
Authors
JournalNeuron
PubMed ID33979633
Exchange of cellular components between platelets and tumor cells: impact on tumor cells behavior.
Authors
JournalTheranostics
PubMed ID35265204
Discovery of chemerin as the new chemoattractant of human mesenchymal stem cells.
Authors
JournalCell Biosci
PubMed ID34210352
Arousal Modulates Retinal Output.
Authors
JournalNeuron
PubMed ID32445624
Endothelial-to-Mesenchymal Transition in Human Adipose Tissue Vasculature Alters the Particulate Secretome and Induces Endothelial Dysfunction.
AuthorsHaynes BA, Yang LF, Huyck RW, Lehrer EJ, Turner JM, Barabutis N, Correll VL, Mathiesen A, McPheat W, Semmes OJ, Dobrian AD
JournalArterioscler Thromb Vasc Biol
PubMed ID31434495
Endothelial cells (EC) in obese adipose tissue (AT) are exposed to a chronic proinflammatory environment that may induce a mesenchymal-like phenotype and altered function. The objective of this study was to establish whether endothelial-to-mesenchymal transition (EndoMT) is present in human AT in obesity and to investigate the effect of such ... More
Extracellular vesicle-packaged HIF-1a-stabilizing lncRNA from tumour-associated macrophages regulates aerobic glycolysis of breast cancer cells.
AuthorsChen F, Chen J, Yang L, Liu J, Zhang X, Zhang Y, Tu Q, Yin D, Lin D, Wong PP, Huang D, Xing Y, Zhao J, Li M, Liu Q, Su F, Su S, Song E
JournalNat Cell Biol
PubMed ID30936474
Metabolic reprogramming is a hallmark of cancer. Here, we demonstrate that tumour-associated macrophages (TAMs) enhance the aerobic glycolysis and apoptotic resistance of breast cancer cells via the extracellular vesicle (EV) transmission of a myeloid-specific lncRNA, HIF-1a-stabilizing long noncoding RNA (HISLA). Mechanistically, HISLA blocks the interaction of PHD2 and HIF-1a to ... More
Leukemia-derived exosomes and cytokines pave the way for entry into the brain.
AuthorsKinjyo I, Bragin D, Grattan R, Winter SS, Wilson BS
JournalJ Leukoc Biol
PubMed ID30702754
Infiltration of acute lymphoblastic leukemia (ALL) blasts into the CNS remains as a major clinical problem, with high risk for chemotherapy-resistant relapse and treatment-related morbidity. Despite the common inclusion of CNS prophylaxis treatments in therapy regimens, there are significant gaps in understanding the mechanisms that mediate leukemia cell entry into ... More
The Temporal Association Cortex Plays a Key Role in Auditory-Driven Maternal Plasticity.
AuthorsTasaka GI, Feigin L, Maor I, Groysman M, DeNardo LA, Schiavo JK, Froemke RC, Luo L, Mizrahi A
JournalNeuron
PubMed ID32473095
Mother-infant bonding develops rapidly following parturition and is accompanied by changes in sensory perception and behavior. Here, we study how ultrasonic vocalizations (USVs) are represented in the brain of mothers. Using a mouse line that allows temporally controlled genetic access to active neurons, we find that the temporal association cortex ... More