Fluorescence microplate-based assay for tumor necrosis factor activity using SYTOX Green stain.
AuthorsJones LJ, Singer VL
JournalAnal Biochem
PubMed ID11373072
'We have developed a simple, sensitive, fluorescence microplate-based assay for tumor necrosis factor (TNF) biological activity. The assay employs SYTOX Green nucleic acid stain to detect TNF-induced cell necrosis in actinomycin D sensitized cultured cell lines. SYTOX Green stain is a cationic unsymmetrical cyanine dye that is excluded from live ... More
In vitro effects of incinerator fly ash on pulmonary macrophages and epithelial cells.
AuthorsDiabaté S, Mülhopt S, Paur HR, Wottrich R, Krug HF
JournalInt J Hyg Environ Health
PubMed ID11885355
Fly ash from a municipal waste incinerator was used as a model for atmospheric particles in order to identify parameters relevant for the induction of adverse health effects. The aim of this study was to compare the biological effects of the total incinerator fly ash (IFA), the soluble and the ... More