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引用和文献 (7)
Invitrogen™
CyQUANT™ 细胞毒性检测试剂盒（G6PD 释放量检测）
CyQUANT 细胞毒性测定试剂盒通过检测损伤和死亡细胞释放到周围培养基中的葡萄糖-6-磷酸脱氢酶来检测损伤和死亡细胞。它可以检测到低至500个细胞，比 LDH 释放量测定试剂盒更加灵敏。本款测定试剂盒采用两步酶法检测葡萄糖-6-磷酸脱氢酶，通过该两步酶法可将刃天青还原为带红色荧光的试卤灵。 产生的荧光信号与释放到细胞培养基中的葡萄糖-6-磷酸脱氢酶的量成比例，且所述释放与样品中死细胞的数量有关。 试卤灵的荧光发射波长了解更多信息
| 货号 | 数量 |
|---|---|
| V23111 | 1000 Assays |
货号 V23111
价格（CNY)
6,628.00
1 kit
数量:
1000 Assays
价格（CNY)
6,628.00
1 kit
CyQUANT 细胞毒性测定试剂盒通过检测损伤和死亡细胞释放到周围培养基中的葡萄糖-6-磷酸脱氢酶来检测损伤和死亡细胞。它可以检测到低至500个细胞，比 LDH 释放量测定试剂盒更加灵敏。
本款测定试剂盒采用两步酶法检测葡萄糖-6-磷酸脱氢酶，通过该两步酶法可将刃天青还原为带红色荧光的试卤灵。 产生的荧光信号与释放到细胞培养基中的葡萄糖-6-磷酸脱氢酶的量成比例，且所述释放与样品中死细胞的数量有关。 试卤灵的荧光发射波长 (ex/em 563/587 nm) 超过大多数生物样品的自发荧光。此外，本款测定试剂盒产生的背景信号比通常在基于乳酸脱氢酶的测定试剂盒中观察到的背景信号更低。
本款测定试剂盒采用两步酶法检测葡萄糖-6-磷酸脱氢酶，通过该两步酶法可将刃天青还原为带红色荧光的试卤灵。 产生的荧光信号与释放到细胞培养基中的葡萄糖-6-磷酸脱氢酶的量成比例，且所述释放与样品中死细胞的数量有关。 试卤灵的荧光发射波长 (ex/em 563/587 nm) 超过大多数生物样品的自发荧光。此外，本款测定试剂盒产生的背景信号比通常在基于乳酸脱氢酶的测定试剂盒中观察到的背景信号更低。
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性Cell-impermeant
检测方法Fluorescence
染料类型其他标记或染料
产品规格96 孔板
数量1000 Assays
运输条件室温
颜色Red
发射587 nm
Excitation Wavelength Range563 nm
适用于（应用）细胞毒性测定试剂盒（G6PD 释放量测定试剂盒）
适用于（设备）微孔板读数仪
产品线CyQUANT
产品类型G6PD Release Cytotoxicity Assay
Unit Size1 kit
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。
常见问题解答 (FAQ)
What are the fluorescence excitation/emission maxima for Resorufin that is generated in the Vybrant Cytotoxicity Assay Kit (G6PD Release Assay)?
How many assays can I perform using the CyQUANT Cytotoxicity Assay Kit (G6PD Release Assay) (Cat. No. V23111)?
Can I use flash-frozen supernatant from the cell culture for the CyQUANT Cytotoxicity Assay (Cat. No. V23111)?
引用和文献 (7)
引用和文献
Abstract
Use of cellular glucose-6-phosphate dehydrogenase for cell quantitation: applications in cytotoxicity and apoptosis assays.
Journal:Anal Biochem
PubMed ID:15136165
'A fluorescence-based microplate assay was developed to quantify cell death based upon the measurement of glucose-6-phosphate dehydrogenase (G6PD) activity. G6PD is a cytosolic enzyme and leaks from cells when plasma membrane integrity is compromised. In this assay, cell death is measured by correlating the activity of extracellular G6PD to the
Development of a comprehensive human immunodeficiency virus type 1 screening algorithm for discovery and preclinical testing of topical microbicides.
Journal:Antimicrob Agents Chemother
PubMed ID:18316528
'Topical microbicides are self-administered, prophylactic products for protection against sexually transmitted pathogens. A large number of compounds with known anti-human immunodeficiency virus type 1 (HIV-1) inhibitory activity have been proposed as candidate topical microbicides. To identify potential leads, an in vitro screening algorithm was developed to evaluate candidate microbicides in
In vivo imaging platform for tracking immunotherapeutic cells.
Journal:Nat Biotechnol
PubMed ID:16041364
Cellular therapeutics show great promise for the treatment of disease, but few noninvasive techniques exist for monitoring the cells after administration. Here we present a magnetic resonance imaging (MRI) technology that uses perfluoropolyether (PFPE) agents to track cells in vivo. Fluorine MRI selectively images only the labeled cells, and a
Kaposi's sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells.
Journal:J Virol
PubMed ID:23536671
Kaposi sarcoma-associated herpesvirus (KSHV) stimulates proliferation, angiogenesis, and inflammation to promote Kaposi sarcoma (KS) tumor growth, which involves various growth factors and cytokines. Previously, we found that KSHV infection of human umbilical vein endothelial cells (HUVECs) induces a transcriptional induction of the proangiogenic and proinflammatory cytokine angiopoietin-2 (Ang-2). Here, we
Extracellular DNA, Neutrophil Extracellular Traps, and Inflammasome Activation in Severe Asthma.
Journal:Am J Respir Crit Care Med
PubMed ID:30888839