Multipotent stem cells from trabecular meshwork become phagocytic TM cells.
AuthorsDu Y, Roh DS, Mann MM, Funderburgh ML, Funderburgh JL, Schuman JS,
JournalInvest Ophthalmol Vis Sci
PubMed ID22297497
'To isolate and characterize stem cells from human trabecular meshwork (TM) and to investigate the potential of these stem cells to differentiate into TM cells. Human trabecular meshwork stem cells (TMSCs) were isolated as side population cells by fluorescence-activated cell sorting or isolated by clonal cultures. Passaged TMSCs were compared ... More
Erythropoiesis in the absence of adult hemoglobin.
AuthorsLiu S, McConnell SC, Ryan TM
JournalMol Cell Biol
PubMed ID23530053
'During erythropoiesis, hemoglobin (Hb) synthesis increases from early progenitors to mature enucleated erythrocytes. Although Hb is one of the most extensively studied proteins, the role of Hb in erythroid lineage commitment, differentiation, and maturation remains unclear. In this study, we generate mouse embryos and embryonic stem (ES) cells with all ... More
An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells.
AuthorsMuslimovic A, Ismail IH, Gao Y, Hammarsten O,
JournalNat Protoc
PubMed ID18600224
'Phosphorylation of histone protein H2AX on serine 139 (gamma-H2AX) occurs at sites flanking DNA double-stranded breaks (DSBs) and can provide a measure of the number of DSBs within a cell. We describe a flow cytometry-based method optimized to measure gamma-H2AX in nonfixed mononuclear blood cells as well as in cultured ... More
Side population analysis using a violet-excited cell-permeable DNA binding dye.
AuthorsTelford WG, Bradford J, Godfrey W, Robey RW, Bates SE
JournalStem Cells
PubMed ID17185610
'Hoechst 33342 side population (SP) analysis is a common method for identifying stem cells in mammalian hematopoietic and nonhematopoietic tissues. Although widely employed for stem cell analysis, this method requires an ultraviolet (UV) laser to excite Hoechst 33342. Flow cytometers equipped with UV sources are not common because of the ... More
Induction of DNA damage response by the supravital probes of nucleic acids.
'The aim of this study was to assess the potential DNA damage response (DDR) to four supravitally used biomarkers Hoechst 33342 (Ho 42), DRAQ5, DyeCycle Violet (DCV), and SYTO 17. A549 cells were exposed to these biomarkers at concentrations generally applied to live cells and their effect on histone H2AX ... More
Cytotoxicity and modes of action of four naturally occuring benzophenones: 2,2',5,6'-tetrahydroxybenzophenone, guttiferone E, isogarcinol and isoxanthochymol.
AuthorsKuete V, Tchakam PD, Wiench B, Ngameni B, Wabo HK, Tala MF, Moungang ML, Ngadjui BT, Murayama T, Efferth T
JournalPhytomedicine
PubMed ID23507522
'The emergence of drug-resistant cancer cells drastically reduces the efficacy of many antineoplasic agents and, consequently, increases the frequency of therapeutic failure. Benzophenones are known to display many pharmacological properties including cytotoxic activities. The present study was aimed at investigating the cytotoxicity and the modes of action of four naturally ... More
Simultaneous prospective purification of adult subventricular zone neural stem cells and their progeny.
AuthorsPastrana E, Cheng LC, Doetsch F,
JournalProc Natl Acad Sci U S A
PubMed ID19332781
'The ability to prospectively isolate adult neural stem cells and their progeny is crucial to study their biology and therapeutic potential. Stem cells in adult mammalian neurogenic niches are a subset of astrocytes. A major limitation in the field has been the inability to distinguish stem cell astrocytes from niche ... More
UV-activated conversion of Hoechst 33258, DAPI, and Vybrant DyeCycle fluorescent dyes into blue-excited, green-emitting protonated forms.
'Hoechst 33258, DAPI and Vybrant DyeCycle are commonly known DNA fluorescent dyes that are excited by UV and emit in the blue region of the spectrum of visible light. Conveniently, they leave the reminder of the spectrum for microscopy detection of other cellular targets labeled with probes emitting in green, ... More
Stem cell identification by DyeCycle Violet side population analysis.
AuthorsTelford WG
JournalMethods Mol Biol
PubMed ID23179832
Hoechst side population (SP) analysis remains a critical technique for identifying stem cell and progenitor populations in hematopoietic and non-hematopoietic tissues, as well as potential cancer stem cells. More recently, DyeCycle Violet (DCV), a DNA binding dye structurally similar to Hoechst 33342 but with an excitation spectrum shifted toward the ... More
A novel human embryonic stem cell-derived Huntington's disease neuronal model exhibits mutant huntingtin (mHTT) aggregates and soluble mHTT-dependent neurodegeneration.
AuthorsLu B, Palacino J
JournalFASEB J
PubMed ID23325320
Most neurodegenerative diseases are linked to aberrant accumulation of aggregation-prone proteins. Among them, Huntington's disease (HD) is caused by an expanded polyglutamine repeat stretch in the N terminus of the mutant huntingtin protein (mHTT), which gets cleaved and aggregates in the brain. Recently established human induced pluripotent stem cell-derived HD ... More