Genome-wide copy number profiling of single cells in S-phase reveals DNA-replication domains.
AuthorsVan der Aa N, Cheng J, Mateiu L, Zamani Esteki M, Kumar P, Dimitriadou E, Vanneste E, Moreau Y, Vermeesch JR, Voet T,
JournalNucleic Acids Res
PubMed ID23295674
'Single-cell genomics is revolutionizing basic genome research and clinical genetic diagnosis. However, none of the current research or clinical methods for single-cell analysis distinguishes between the analysis of a cell in G1-, S- or G2/M-phase of the cell cycle. Here, we demonstrate by means of array comparative genomic hybridization that ... More
Lack of p21 expression links cell cycle control and appendage regeneration in mice.
AuthorsBedelbaeva K, Snyder A, Gourevitch D, Clark L, Zhang XM, Leferovich J, Cheverud JM, Lieberman P, Heber-Katz E,
JournalProc Natl Acad Sci U S A
PubMed ID20231440
'Animals capable of regenerating multiple tissue types, organs, and appendages after injury are common yet sporadic and include some sponge, hydra, planarian, and salamander (i.e., newt and axolotl) species, but notably such regenerative capacity is rare in mammals. The adult MRL mouse strain is a rare exception to the rule ... More
Classic
AuthorsHenderson L, Bortone DS, Lim C, Zambon AC,
JournalAm J Physiol Cell Physiol
PubMed ID23392113
'Many common, important diseases are either caused or exacerbated by hyperactivation (e.g., cancer) or inactivation (e.g., heart failure) of the cell division cycle. A better understanding of the cell cycle is critical for interpreting numerous types of physiological changes in cells. Moreover, new insights into how to control it will ... More
Genome duplication and mutations in ACE2 cause multicellular, fast-sedimenting phenotypes in evolved Saccharomyces cerevisiae.
AuthorsOud B, Guadalupe-Medina V, Nijkamp JF, de Ridder D, Pronk JT, van Maris AJ, Daran JM,
Journal
PubMed ID24145419
'Laboratory evolution of the yeast Saccharomyces cerevisiae in bioreactor batch cultures yielded variants that grow as multicellular, fast-sedimenting clusters. Knowledge of the molecular basis of this phenomenon may contribute to the understanding of natural evolution of multicellularity and to manipulating cell sedimentation in laboratory and industrial applications of S. cerevisiae. ... More
Essential role for Dnmt1 in the prevention and maintenance of MYC-induced T-cell lymphomas.
AuthorsPeters SL, Hlady RA, Opavska J, Klinkebiel D, Novakova S, Smith LM, Lewis RE, Karpf AR, Simpson MA, Wu L, Opavsky R,
Journal
PubMed ID24001767
'DNA cytosine methylation is an epigenetic modification involved in the transcriptional repression of genes controlling a variety of physiological processes, including hematopoiesis. DNA methyltransferase 1 (Dnmt1) is a key enzyme involved in the somatic inheritance of DNA methylation and thus plays a critical role in epigenomic stability. Aberrant methylation contributes ... More
Feedback control of DnaA-mediated replication initiation by replisome-associated HdaA protein in Caulobacter.
AuthorsCollier J, Shapiro L,
JournalJ Bacteriol
PubMed ID19633089
'Chromosome replication in Caulobacter crescentus is tightly regulated to ensure that initiation occurs at the right time and only once during the cell cycle. The timing of replication initiation is controlled by both CtrA and DnaA. CtrA binds to and silences the origin. Upon the clearance of CtrA from the ... More
Two separate defects affecting true naive or virtual memory T cell precursors combine to reduce naive T cell responses with aging.
AuthorsRenkema KR, Li G, Wu A, Smithey MJ, Nikolich-Žugich J,
Journal
PubMed ID24293630
Naive T cell responses are eroded with aging. We and others have recently shown that unimmunized old mice lose = 70% of Ag-specific CD8 T cell precursors and that many of the remaining precursors acquire a virtual (central) memory (VM; CD44(hi)CD62L(hi)) phenotype. In this study, we demonstrate that unimmunized TCR ... More
The Caulobacter crescentus ctrA P1 promoter is essential for the coordination of cell cycle events that prevent the overinitiation of DNA replication.
AuthorsSchredl AT, Perez Mora YG, Herrera A, Cuajungco MP, Murray SR,
JournalMicrobiology
PubMed ID22790399
The master regulator CtrA oscillates during the Caulobacter cell cycle due to temporally regulated proteolysis and transcription. It is proteolysed during the G1-S transition and reaccumulates in predivisional cells as a result of transcription from two sequentially activated promoters, P1 and P2. CtrA reinforces its own synthesis by directly mediating ... More
The RAD23 family provides an essential connection between the 26S proteasome and ubiquitylated proteins in Arabidopsis.
AuthorsFarmer LM, Book AJ, Lee KH, Lin YL, Fu H, Vierstra RD,
JournalPlant Cell
PubMed ID20086187
The ubiquitin (Ub)/26S proteasome system (UPS) directs the turnover of numerous regulatory proteins, thereby exerting control over many aspects of plant growth, development, and survival. The UPS is directed in part by a group of Ub-like/Ub-associated (UBL/UBA) proteins that help shuttle ubiquitylated proteins to the 26S proteasome for breakdown. Here, ... More
SpoT regulates DnaA stability and initiation of DNA replication in carbon-starved Caulobacter crescentus.
AuthorsLesley JA, Shapiro L,
JournalJ Bacteriol
PubMed ID18723629
Cell cycle progression and polar differentiation are temporally coordinated in Caulobacter crescentus. This oligotrophic bacterium divides asymmetrically to produce a motile swarmer cell that represses DNA replication and a sessile stalked cell that replicates its DNA. The initiation of DNA replication coincides with the proteolysis of the CtrA replication inhibitor ... More
Separate functions for nuclear and cytoplasmic cryptochrome 1 during photomorphogenesis of Arabidopsis seedlings.
AuthorsWu G, Spalding EP,
JournalProc Natl Acad Sci U S A
PubMed ID18003924
Cryptochrome blue-light receptors mediate many aspects of plant photomorphogenesis, such as suppression of hypocotyl elongation and promotion of cotyledon expansion and root growth. The cryptochrome 1 (cry1) protein of Arabidopsis is present in the nucleus and cytoplasm of cells, but how the functions of one pool differ from the other ... More
Mitochondrial Staining Allows Robust Elimination of Apoptotic and Damaged Cells during Cell Sorting.
AuthorsBarteneva NS, Ponomarev ED, Tsytsykova A, Armant M, Vorobjev IA,
Journal
PubMed ID24394470
High-speed fluorescence-activated cell sorting is relevant for a plethora of applications, such as PCR-based techniques, microarrays, cloning, and propagation of selected cell populations. We suggest a simple cell-sorting technique to eliminate early and late apoptotic and necrotic cells, with good signal-to-noise ratio and a high-purity yield. The mitochondrial potential dye, ... More
The catalytic subunit of Drosophila glutamate-cysteine ligase is a nucleocytoplasmic shuttling protein.
GSH concentration is considerably lower in the nucleus than in the cytoplasm; however, it is significantly elevated during active cell proliferation. The main purpose of this study was to understand the mechanism underlying these variations in nuclear/cytoplasmic distribution of GSH. The rate-limiting step in the de novo GSH biosynthesis pathway ... More
Hyperspectral image analysis of live cells in various cell cycle stages.
AuthorsDicker DT, Lerner JM, El-Deiry WS,
JournalCell Cycle
PubMed ID17912031
In this study we have explored the use of hyperspectral imaging (HSI) to determine the cell cycle status of live cells in culture. Live cancer cell lines in culture were either synchronized by release from nocodazole or arrested in various cell-cycle phases with serum starvation (G1), aphidicolin (S), or nocodazole ... More
SYTO probes in the cytometry of tumor cell death.
AuthorsWlodkowic D, Skommer J, Darzynkiewicz Z,
JournalCytometry A
PubMed ID18260152
Apoptosis is a complex and finely controlled cell death process of great relevance in tissue homeostasis and pathogenesis. The majority of classical apoptotic features can be examined by flow as well as image cytometry. Therefore, cytometry has been used as a technology of choice in studies of tumor cell demise. ... More