Identification and characterization of galectin-9, a novel beta- galactoside-binding mammalian lectin.
AuthorsWada J, Kanwar YS
JournalJ Biol Chem
PubMed ID9038233
'A 36-kDa beta-galactoside mammalian lectin protein, designated as galectin-9, was isolated from mouse embryonic kidney by using a degenerate primer polymerase chain reaction and cloning strategy. Its deduced amino acid sequence had the characteristic conserved sequence motif of galectins. Endogenous galectin-9, extracted from liver and thymus, as well as recombinant ... More
A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export.
AuthorsKehlenbach RH, Dickmanns A, Kehlenbach A, Guan T, Gerace L
JournalJ Cell Biol
PubMed ID10330396
'We recently developed an assay in which nuclear export of the shuttling transcription factor NFAT (nuclear factor of activated T cells) can be reconstituted in permeabilized cells with the GTPase Ran and the nuclear export receptor CRM1. We have now used this assay to identify another export factor. After preincubation ... More
HSP70-2 is required for CDC2 kinase activity in meiosis I of mouse spermatocytes [published erratum appears in Development 1997 Sep;134(17):3218]
AuthorsZhu D, Dix DJ, Eddy EM
JournalDevelopment
PubMed ID9247342
'Cyclin B-dependent CDC2 kinase activity has a key role in triggering the G2/M-phase transition during the mitotic and meiotic cell cycles. The Hsp70-2 gene is expressed only in spermatogenic cells at a significant level. In Hsp70-2 gene knock-out (Hsp70-2(-/-)) mice, primary spermatocytes fail to complete meiosis I, suggesting a link ... More
Activation mechanism of Gi and Go by reactive oxygen species.
Reactive oxygen species are proposed to work as intracellular mediators. One of their target proteins is the alpha subunit of heterotrimeric GTP-binding proteins (Galpha(i) and Galpha(o)), leading to activation. H(2)O(2) is one of the reactive oxygen species and activates purified Galpha(i2). However, the activation requires the presence of Fe(2+), suggesting ... More
Galectin-7 (PIG1) exhibits pro-apoptotic function through JNK activation and mitochondrial cytochrome c release.
Authors Kuwabara Ichiro; Kuwabara Yasuko; Yang Ri-Yao; Schuler Martin; Green Douglas R; Zuraw Bruce L; Hsu Daniel K; Liu Fu-Tong;
JournalJ Biol Chem
PubMed ID11706006
Galectin-7 is normally expressed in all types of stratified epithelia, but is significantly down-regulated in squamous cell carcinomas. This protein was recently found to be highly inducible by p53 in a colon carcinoma cell line, DLD-1, and designated as PIG1 (for p53-induced gene 1). We studied transfectants of HeLa and ... More