pLenti6.2-GW/EmGFP Expression Control Vector

Invitrogen™

pLenti6.2-GW/EmGFP Expression Control Vector

The Vivid Colors™ pLenti6.2-GW⁄EmGFP Expression Control Vector is a ViraPower™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP).Read more

Catalog Number	Quantity
V36920 also known as V369-20	1 Ea.

Catalog number V36920

also known as V369-20

Price (CNY)

30,477.00

Online Exclusive

Ends: 31-Dec-2026

35,644.00

Save 5,167.00 (14%)

1 each

Add to cart

Quantity:

1 Ea.

Price (CNY)

30,477.00

Online Exclusive

Ends: 31-Dec-2026

35,644.00

Save 5,167.00 (14%)

1 each

Add to cart

The Vivid Colors™ pLenti6.2-GW⁄EmGFP Expression Control Vector is a ViraPower™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP). It is designed for use with the ViraPower™ Lentiviral Expression Systems as a positive control to enable the detection of EmGFP fluorescence following transfection in 293FT cells, as a titer control to produce an EmGFP-expressing lentivirus stock and as a transduction control following transduction in both dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of EmGFP and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker. This is not a cloning vector.

Advantages
• Optimization of viral transduction efficiency
• Optimization of 293FT Transfection
• 2 day titer of functional virus using EmGFP

Key Features
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the EmGFP
• PKG Promoter for expression of Blasticidin selection marker

Kit Includes
pLenti6.2-GW⁄EmGFP Vector

Related SKUs
• 293FT Cell Line (R70007; R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine™ 2000 (11668-019; 11668-027)

For research use only. Not intended for any therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Constitutive or Inducible SystemConstitutive

Delivery TypeLentiviral

For Use With (Application)Reporter Assays, Viral Expression

Product TypeLentiviral Expression Vector

Quantity1 Ea.

Reporter GeneGFP (EmGFP)

Selection Agent (Eukaryotic)Blasticidin

Selection Marker PromoterPGK Promoter

VectorpLenti

Cloning MethodGateway

Product LineGateway, ViraPower, Vivid Colors

PromoterCMV

Protein TagUntagged

Unit Size1 each

Contents & Storage

pLenti6.2-GW⁄EmGFP Vector: 20 μg of control vector is supplied in solution as 40 μl of 0.5 μg⁄μl in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. Store at –20°C.

Frequently asked questions (FAQs)

I used your pLenti6.2-GW/EmGFP Control Vector and got poor expression of EmGFP after stable transduction into my cell line. What should I do?

This can happen due to excess blasticidin used during selection. Determine the antibiotic sensitivity of your cell line by performing a kill curve. Use the minimum antibiotic concentration required to kill your untransduced cell line.

I used your pLenti6.2-GW/EmGFP Control Vector and got no expression of EmGFP after stable transduction into my cell line. Can you please help?

Promoter silencing or incorrect filter used/detection parameters for flow cytometer are incorrect could lead to no epxression of EmGFP after stable transduction. Screen for multiple antibiotic-resistant clones and select the one with the highest expression levels. Make sure the correct filter is used as well as the FITC detection parameters.

I used your pLenti6.2-GW/EmGFP Control Vector and got poor expression of EmGFP after transient transduction into my cell line. Can you please help?

Poor expression could result from low transduction efficiency, too low of a MOI, or cells harvested too soon after tranduction. Ensure that cells are tranduced in the presence of Polybrene reagent. Check the MOI and/or use a higher MOI, and do not harvest cells until 48-72 hrs post-tranduction.

I used your pLenti6.2-GW/EmGFP Control Vector and got no EmGFP-positive cells after titering. What could have happened?

Here are some possible causes and solutions:

- Incorrect filter or incorrect detection parameters for flow cytommetry; make sure you are using a FITC or Omega XF100 filter set on yoru inverted fluorescence microscope or the FITC detection parameters on your flow cytometer.
- Viral stock stored incorrectly; aliquot and store at -80 degrees C, do not freeze/thaw more than 3 times.
- Polybrene reagent not included during transduction; transduce pLenti6.2-GW/EmGFP into cells in the presence of Polybrene reagent
- Too soon to see EmGFP expression; wait 4 days post-tranduction

How large of a PCR product can I recombine with a pDONR vector via BP cloning? Does the same apply for TOPO-adapted Entry vectors?

There is no theoretical limit to insert size for a BP reaction with a pDONR vector. Maximum size tested in-house is 12 kb. TOPO vectors are more sensitive to insert size and 3-5 kb is the upper limit for decent cloning efficiency.

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