Lymphocyte-specific protein 1 expression in eukaryotic cells reproduces the morphologic and motile abnormality of NAD 47/89 neutrophils.
AuthorsHoward TH, Hartwig J, Cunningham C
JournalBlood
PubMed ID9616178
'Despite its name, the actin-binding protein lymphocyte-specific protein1 (LSP1) is found in all hematopoetic cells, and yet its role in cell function remains unclear. Recently, LSP1 was identified as the 47- kD protein overexpressed in the polymorphonuclear neutrophils of patients with a rare neutrophil disorder, neutrophil actin dysfunction with abnormalities ... More
24-hour assay for estimating the titer of beta-galactosidase-expressing baculovirus.
AuthorsSussman DJ
JournalBiotechniques
PubMed ID7702852
No abstract
ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor [see comments]
AuthorsTsukiyama T, Becker PB, Wu C
JournalNature
PubMed ID8107823
Genetic control elements are usually situated in local regions of chromatin that are hypersensitive to structural probes such as DNase I. We have reconstructed the chromatin structure of the hsp70 promoter using an in vitro nucleosome assembly system. Binding of the GAGA transcription factor on existing nucleosomes leads to nucleosome ... More
The ubiquitous chromatin protein DEK alters the structure of DNA by introducing positive supercoils.
We have investigated the molecular mechanism by which the proto-oncogene protein DEK, an abundant chromatin-associated protein, changes the topology of DNA in chromatin in vitro. Band-shift assays and electron microscopy revealed that DEK induces both intra- and intermolecular interactions between DNA molecules. Binding of the DEK protein introduces constrained positive ... More
IKAP is a scaffold protein of the IkappaB kinase complex.
AuthorsCohen L, Henzel WJ, Baeuerle PA
JournalNature
PubMed ID9751059
The transcription factor NF-kappaB coordinates the activation of numerous genes in response to pathogens andpro-inflammatory cytokines, and is, therefore, vital in the development of acute and chronic inflammatory diseases. NF-kappaB is activated by phsophorylation of its inhibitory subunit, IkappaB-alpha, on serine residues 32 and 36 by cytokine-activated IKB kinases (IKKs); ... More
Bop1 is a mouse WD40 repeat nucleolar protein involved in 28S and 5. 8S RRNA processing and 60S ribosome biogenesis
AuthorsStrezoska Z, Pestov DG, Lau LF
JournalMol Cell Biol
PubMed ID10891491
We have identified and characterized a novel mouse protein, Bop1, which contains WD40 repeats and is highly conserved through evolution. bop1 is ubiquitously expressed in all mouse tissues examined and is upregulated during mid-G(1) in serum-stimulated fibroblasts. Immunofluorescence analysis shows that Bop1 is localized predominantly to the nucleolus. In sucrose ... More
RYK, a catalytically inactive receptor tyrosine kinase, associates with EphB2 and EphB3 but does not interact with AF-6.
Authors Trivier Elisabeth; Ganesan Trivadi S;
JournalJ Biol Chem
PubMed ID11956217
RYK is an atypical orphan receptor tyrosine kinase that lacks detectable kinase activity. Nevertheless, using a chimeric receptor approach, we previously found that RYK can signal via the mitogen-activated protein kinase pathway. Recently, it has been shown that murine Ryk can bind to and be phosphorylated by the ephrin receptors ... More