LMP-1, a LIM-domain protein, mediates BMP-6 effects on bone formation.
AuthorsBoden SD, Liu Y, Hair GA, Helms JA, Hu D, Racine M, Nanes MS, Titus L
JournalEndocrinology
PubMed ID9832452
Glucocorticoids can promote osteoblast differentiation from fetal calvarial cells and bone marrow stromal cells. We recently reported that glucocorticoid specifically induced bone morphogenetic protein-6 (BMP-6), a glycoprotein signaling molecule that is a multifunctional regulator of vertebrate development. In the present study, we used fetal rat secondary calvarial cultures to determine ... More
DBC2, a candidate for a tumor suppressor gene involved in breast cancer.
AuthorsHamaguchi Masaaki; Meth Jennifer L; von Klitzing Christine; Wei Wen; Esposito Diane; Rodgers Linda; Walsh Tom; Welcsh Piri; King Mary-Claire; Wigler Michael H;
JournalProc Natl Acad Sci U S A
PubMed ID12370419
A previously uncharacterized gene, DBC2 (deleted in breast cancer), was cloned from a homozygously deleted region at human chromosome 8p21. DBC2 contains a highly conserved RAS domain and two putative protein interacting domains. Our analyses indicate that DBC2 is the best candidate tumor suppressor gene from this region. It lies ... More
Characterization and channel coupling of the P2Y(12) nucleotide receptor of brain capillary endothelial cells.
AuthorsSimon Joseph; Filippov Alexander K; Göransson Sara; Wong Yung H; Frelin Christian; Michel Anton D; Brown David A; Barnard Eric A;
JournalJ Biol Chem
PubMed ID12080041
Rat brain capillary endothelial (B10) cells express an unidentified nucleotide receptor linked to adenylyl cyclase inhibition. We show that this receptor in B10 cells is identical in sequence to the P2Y(12) ADP receptor ( ... More
Rab11-FIP2 functions in transferrin recycling and associates with endosomal membranes via its COOH-terminal domain.
Authors Lindsay Andrew J; McCaffrey Mary W;
JournalJ Biol Chem
PubMed ID11994279
'Rab11-FIP2 is a recently described member of the Rip11/Rab11-FIP/Rab coupling protein family of Rab11 interacting proteins. Rab11-FIP2 interacts with both Rab11 and myosin Vb and co-localizes with Rab11 in both HeLa and Madin-Darby canine kidney cells (Hales, C. M., Griner, R., Hobdy-Henderson, K. C., Dorn, M. C., Hardy, D., Kumar, ... More
N-terminal processing is essential for release of epithin, a mouse type II membrane serine protease.
Authors Cho E G; Kim M G; Kim C; Kim S R; Seong I S; Chung C; Schwartz R H; Park D;
JournalJ Biol Chem
PubMed ID11567025
'Epithin was originally identified as a mouse type II membrane serine protease. Its human orthologue membrane type-serine protease 1 (MT-SP1)/matriptase has been reported to be localized on the plasma membrane. In addition, soluble forms of matriptase were isolated from human breast milk and breast cancer cell-conditioned medium. In this paper, ... More
Activation of macrophages by linear (1right-arrow3)-beta-D-glucans. Impliations for the recognition of fungi by innate immunity.
'Although (1-->3)-beta-d-glucans, which are one of major fungal cell wall components, are known to activate invertebrate innate immune systems, their activities on mammalian cells remain elusive. Here, we report their activities on mouse macrophages. Among the various (1-->3)-beta-d-glucans, curdlan, a linear (1-->3)-beta-d-glucan, although not branched beta-glucans, exhibits significant activity to ... More
Caspase-2 can trigger cytochrome C release and apoptosis from the nucleus.
'The cysteine proteases specific for aspartic residues, known as caspases, are localized in different subcellular compartments and play specific roles during the regulative and the executive phase of the cell death process. Here we investigated the subcellular localization of caspase-2 in healthy cells and during the execution of the apoptotic ... More
Mouse transferrin receptor 1 is the cell entry receptor for mouse mammary tumor virus.
Authors Ross Susan R; Schofield Jason J; Farr Christine J; Bucan Maja;
JournalProc Natl Acad Sci U S A
PubMed ID12218182
'Enveloped viruses enter cells by binding to their entry receptors and fusing with the membrane at the cell surface or after trafficking through acidic endosomal compartments. Species-specific virus tropism is usually determined by these entry receptors. Because mouse mammary tumor virus (MMTV) is unable to infect Chinese hamster cells, we ... More
The interaction between the I-II loop and the III-IV loop of Cav2.1 contributes to voltage-dependent inactivation in a beta -dependent manner.
'We have investigated the molecular mechanisms whereby the I-II loop controls voltage-dependent inactivation in P/Q calcium channels. We demonstrate that the I-II loop is localized in a central position to control calcium channel activity through the interaction with several cytoplasmic sequences; including the III-IV loop. Several experiments reveal the crucial ... More
Identification of a novel human eicosanoid receptor coupled to G(i/o).
'We have conducted an in silico data base search for and cloned a novel G-protein-coupled receptor (GPCR) named TG1019. Dot and Northern blotting analyses showed that transcripts of the novel GPCR were expressed in various tissues except brain, and the expression was more intense in liver, kidney, peripheral leukocyte, lung, ... More
The human organic cation transporter (hOCT2) recognizes the degree of substrate ionization.
Authors Barendt Wendy M; Wright Stephen H;
JournalJ Biol Chem
PubMed ID11953440
'The organic cation transporter, OCT2, plays a role in renal secretion of a broad array of weak bases. To determine whether the degree of ionization of these compounds plays a role in their interaction with OCT2, we examined the influence of external pH values on the activity of the human ... More
Dexras1/AGS-1 inhibits signal transduction from the Gi-coupled formyl peptide receptor to Erk-1/2 MAP kinases.
Authors Graham Timothy E; Prossnitz Eric R; Dorin Richard I;
JournalJ Biol Chem
PubMed ID11751935
'Dexras1 is a novel GTP-binding protein (G protein) that was recently discovered on the basis of rapid mRNA up-regulation by glucocorticoids in murine AtT-20 corticotroph cells and in several primary tissues. The human homologue of Dexras1, termed activator of G protein signaling-1 (AGS-1), has been reported to stimulate signaling by ... More
Selective binding of synapse-associated protein 97 to GluR-A alpha-amino-5-hydroxy-3-methyl-4-isoxazole propionate receptor subunit is determined by a novel sequence motif.
Authors Cai Chunlin; Coleman Sarah K; Niemi Katri; Keinänen Kari;
JournalJ Biol Chem
PubMed ID12070168
'A family of four closely related PDZ domain-containing membrane-associated guanylate kinase homologues (MAGUKs) is involved in the regulation of the amount and functional state of ionotropic glutamate receptors in excitatory synapses. To understand the mechanisms that determine the specificity of these interactions, we examined the structural basis of the highly ... More
CTCF mediates methylation-sensitive enhancer-blocking activity at the H19/Igf2 locus.
'The Insulin-like growth factor 2 (Igf2) and H19 genes are imprinted, resulting in silencing of the maternal and paternal alleles, respectively. This event is dependent upon an imprinted-control region two kilobases upstream of H19 (refs 1, 2). On the paternal chromosome this element is methylated and required for the silencing ... More
The Novel Role of the C-terminal Region of SHP-2. INVOLVEMENT OF Gab1 AND SHP-2 PHOSPHATASE ACTIVITY IN Elk-1 ACTIVATION.
Authors Huang Qunhua; Lerner-Marmarosh Nicole; Che Wenyi; Ohta Shinsuke; Osawa Masaki; Yoshizumi Masanori; Glassman Michael; Yan Chen; Berk Bradford C; Abe Jun-Ichi;
JournalJ Biol Chem
PubMed ID12011040
'SHP-2, a nontransmembrane-type protein-tyrosine phosphatase that contains two Src homology 2 (SH2) domains, is thought to participate in growth factor signal transduction pathways via SH2 domain interactions. To determine the role of each region of SHP-2 in platelet-derived growth factor signaling assayed by Elk-1 activation, we generated six deletion mutants ... More
Identification and characterization of Gemin7, a novel component of the survival of motor neuron complex.
'The survival of motor neurons (SMN) protein is the product of the gene mutated or deleted in the neurodegenerative disease, spinal muscular atrophy. SMN is part of a large macromolecular complex that also contains Gemin2, Gemin3, Gemin4, Gemin5, and Gemin6. The SMN complex functions in the assembly of spliceosomal small ... More
Orphan G protein-coupled receptors MrgA1 and MrgC11 are distinctively activated by RF-amide-related peptides through the Galpha q/11 pathway.
Authors Han Sang-Kyou; Dong Xinzhong; Hwang Jong-Ik; Zylka Mark J; Anderson David J; Simon Melvin I;
JournalProc Natl Acad Sci U S A
PubMed ID12397184
'MrgA1 and MrgC11 belong to a recently identified family of orphan G-protein coupled receptors, called mrgs (mas-related genes). They are only expressed in a specific subset of sensory neurons that are known to detect painful stimuli. However, the precise physiological function of Mrg receptors and their underlying mechanisms of signal ... More
Bifocal is a downstream target of the Ste20-like serine/threonine kinase misshapen in regulating photoreceptor growth cone targeting in Drosophila.
AuthorsRuan W, Long H, Vuong DH, Rao Y,
JournalNeuron
PubMed ID12467587
'Misshapen (Msn) has been proposed to shut down Drosophila photoreceptor (R cell) growth cone motility in response to targeting signals linked by the SH2/SH3 adaptor protein Dock. Here, we show that Bifocal (Bif), a putative cytoskeletal regulator, is a component of the Msn pathway for regulating R cell growth cone ... More
The p53-activated gene, PAG608, requires a zinc finger domain for nuclear localization and oxidative stress-induced apoptosis.
'The p53-activated gene PAG608, which encodes a nuclear zinc finger protein, is a p53-inducible gene that contributes to p53-mediated apoptosis. However, the mechanisms by which PAG608 is involved in the apoptosis of neuronal cells are still obscure. In this study, we demonstrated that expression of p53 was induced by 100 ... More
Redox regulation of Cdc25C.
Authors Savitsky Pavel A; Finkel Toren;
JournalJ Biol Chem
PubMed ID11925443
'The Cdc25 family of dual specific phosphatases are critical components of cell cycle progression and checkpoint control. Certain stresses such as ultraviolet light stimulate the rapid and selective destruction of Cdc25A protein through a Chk1 protein kinase-dependent pathway. We demonstrate that in contrast to cellular stresses previously examined, hydrogen peroxide ... More
Active site residues of cyclophilin A are crucial for its signaling activity via CD147.
'Cyclophilin A (CyPA), a ubiquitously distributed intracellular protein, is a peptidylprolyl cis-trans-isomerase and the major target of the potent immunosuppressive drug cyclosporin A. Although expressed predominantly as an intracellular molecule, CyPA is secreted by cells in response to inflammatory stimuli and is a potent neutrophil and eosinophil chemoattractant in vitro ... More
The cerebellar leucine-rich acidic nuclear protein interacts with ataxin-1.
AuthorsMatilla A, Koshy BT, Cummings CJ, Isobe T, Orr HT, Zoghbi HY
JournalNature
PubMed ID9353121
'Spinocerebellar ataxia type 1 (SCA1) is an autosomal dominant neurodegenerative disorder characterized by ataxia, progressive motor deterioration, and loss of cerebellar Purkinje cells. SCA1 belongs to a growing group of neurodegenerative disorders caused by expansion of CAG repeats, which encode glutamine. Although the proteins containing these repeats are widely expressed, ... More
Activator of G-protein signaling 1 blocks GIRK channel activation by a G-protein-coupled receptor: apparent disruption of receptor signaling complexes.
Authors Takesono Aya; Nowak Mark W; Cismowski Mary; Duzic Emir; Lanier Stephen M;
JournalJ Biol Chem
PubMed ID11842095
'The Ras-related protein, activator of G-protein signaling 1 (AGS1) or Dexras1, interacts with G(i)/G(o)alpha and activates heterotrimeric G-protein signaling systems independent of a G-protein-coupled receptor (GPCR). As an initial approach to further define the cellular role of AGS1 in GPCR signaling, we determined the influence of AGS1 on the regulation ... More
Modulation of Cardiac Growth and Development by HOP, an Unusual Homeodomain Protein.
Authors Shin Chong; Liu Zhi; Passier Robert; Zhang Chun; Wang Da; Harris Thomas; Yamagishi Hiroyuki; Richardson James; Childs Geoffrey; Olson Eric;
JournalCell
PubMed ID12297046
We have discovered an unusual homeodomain protein, called HOP, which is comprised simply of a homeodomain. HOP is highly expressed in the developing heart where its expression is dependent on the cardiac-restricted homeodomain protein Nkx2.5. HOP does not bind DNA and acts as an antagonist of serum response factor (SRF), ... More
Causal Relationship between the Loss of RUNX3 Expression and Gastric Cancer.
Authors Li Qing Lin; Ito Kosei; Sakakura Chohei; Fukamachi Hiroshi; Inoue Ken ichi; Chi Xin Zi; Lee Kwang Youl; Nomura Shintaro; Lee Chang Woo; Han Sang Bae; Kim Hwan Mook; Kim Wun Jae; Yamamoto Hiromitsu; Yamashita Namiko; Yano Takashi; Ikeda Toshio; Itohara Shigeyoshi; Inazawa Johji; Abe Tatsuo; Hagiwara Akeo; Yamagishi Hisakazu; Ooe Asako; Kaneda Atsushi; Sugimura Takashi; Ushijima Toshikazu; Bae Suk Chul; Ito Yoshiaki;
JournalCell
PubMed ID11955451
Runx3/Pebp2alphaC null mouse gastric mucosa exhibits hyperplasias due to stimulated proliferation and suppressed apoptosis in epithelial cells, and the cells are resistant to growth-inhibitory and apoptosis-inducing action of TGF-beta, indicating that Runx3 is a major growth regulator of gastric epithelial cells. Between 45% and 60% of human gastric cancer cells ... More
Molecular basis for species-specific sensitivity to
Authors Jordt Sven Eric; Julius David;
JournalCell
PubMed ID11853675
Chili peppers produce the pungent vanilloid compound capsaicin, which offers protection from predatory mammals. Birds are indifferent to the pain-producing effects of capsaicin and therefore serve as vectors for seed dispersal. Here, we determine the molecular basis for this species-specific behavioral response by identifying a domain of the rat vanilloid ... More
Polypyrimidine tract-binding protein positively regulates inclusion of an alternative 3'-terminal exon
AuthorsLou H, Helfman DM, Gagel RF, Berget SM
JournalMol Cell Biol
PubMed ID9858533
Polypyrimidine tract-binding protein (PTB) is an abundant vertebrate hnRNP protein. PTB binding sites have been found within introns both upstream and downstream of alternative exons in a number of genes that are negatively controlled by the binding of PTB. We have previously reported that PTB binds to a pyrimidine tract ... More
The DCC gene product induces apoptosis by a mechanism requiring receptor proteolysis.
AuthorsMehlen P, Rabizadeh S, Snipas SJ, Assa-Munt N, Salvesen GS, Bredesen DE
JournalNature
PubMed ID9796814
The development of colonic carcinoma is associated with the mutation of a specific set of genes. One of these, DCC (deleted in colorectal cancer), is a candidate tumour-suppressor gene, and encodes a receptor for netrin-1, a molecule involved in axon guidance. Loss of DCC expression in tumours is not restricted ... More
Molecular cloning of FOG-2: a modulator of transcription factor GATA-4 in cardiomyocytes.
AuthorsSvensson EC, Tufts RL, Polk CE, Leiden JM
JournalProc Natl Acad Sci U S A
PubMed ID9927675
GATA transcription factors are important regulators of both hematopoiesis (GATA-1/2/3) and cardiogenesis (GATA-4) in mammals. The transcriptional activities of the GATA proteins are modulated by their interactions with other transcription factors and with transcriptional coactivators and repressors. Recently, two related zinc finger proteins, U-shaped (USH) and Friend of GATA-1 (FOG) ... More
Crx, a novel Otx-like paired-homeodomain protein, binds to and transactivates photoreceptor cell-specific genes.
AuthorsChen S, Wang QL, Nie Z, Sun H, Lennon G, Copeland NG, Gilbert DJ, Jenkins NA, Zack DJ
JournalNeuron
PubMed ID9390516
The otd/Otx gene family encodes paired-like homeodomain proteins that are involved in the regulation of anterior head structure and sensory organ development. Using the yeast one-hybrid screen with a bait containing the Ret 4 site from the bovine rhodopsin promoter, we have cloned a new member of the family, Crx ... More
Regulation of alternative polyadenylation by U1 snRNPs and SRp20.
AuthorsLou H, Neugebauer KM, Gagel RF, Berget SM
JournalMol Cell Biol
PubMed ID9710581
Although considerable information is currently available about the factors involved in constitutive vertebrate polyadenylation, the factors and mechanisms involved in facilitating communication between polyadenylation and splicing are largely unknown. Even less is known about the regulation of polyadenylation in genes in which 3'-terminal exons are alternatively recognized. Here we demonstrate ... More
Uncoupling ceramide glycosylation by transfection of glucosylceramide synthase antisense reverses adriamycin resistance
AuthorsLiu YY, Han TY, Giuliano AE, Hansen N, Cabot MC
JournalJ Biol Chem
PubMed ID10702281
Previous work from our laboratory demonstrated that increased competence to glycosylate ceramide conferred adriamycin resistance in MCF-7 breast cancer cells (Liu, Y. Y., Han, T. Y., Giuliano, A. E. , and M. C. Cabot. (1999) J. Biol. Chem. 274, 1140-1146). This was achieved by cellular transfection with glucosylceramide synthase (GCS), ... More
5' phospholipid phosphatase SHIP-2 causes protein kinase B inactivation and cell cycle arrest in glioblastoma cells.
AuthorsTaylor V, Wong M, Brandts C, Reilly L, Dean NM, Cowsert LM, Moodie S, Stokoe D
JournalMol Cell Biol
PubMed ID10958682
The tumor suppressor protein PTEN is mutated in glioblastoma multiform brain tumors, resulting in deregulated signaling through the phosphoinositide 3-kinase (PI3K)-protein kinase B (PKB) pathway, which is critical for maintaining proliferation and survival. We have examined the relative roles of the two major phospholipid products of PI3K activity, phosphatidylinositol 3,4-biphosphate ... More
The eukaryotic DNMT2 genes encode a new class of cytosine-5 DNA methyltransferases.
AuthorsTang LY, Reddy MN, Rasheva V, Lee TL, Lin MJ, Hung MS, Shen CK,
JournalJ Biol Chem
PubMed ID12819212
DNMT2 is a subgroup of the eukaryotic cytosine-5 DNA methyltransferase gene family. Unlike the other family members, proteins encoded by DNMT2 genes were not known before to possess DNA methyltransferase activities. Most recently, we have shown that the genome of Drosophila S2 cells stably expressing an exogenous Drosophila dDNMT2 cDNA ... More
Initiation of hepatitis delta virus genome replication
AuthorsDingle K, Bichko V, Zuccola H, Hogle J, Taylor J
JournalJ Virol
PubMed ID9573243
The small, 195-amino-acid form of the hepatitis delta virus (HDV) antigen (deltaAg-S) is essential for genome replication, i.e., for the transcription, processing, and accumulation of HDV RNAs. To better understand this requirement, we used purified recombinant deltaAg-S and HDV RNA synthesized in vitro to assemble high-molecular-weight ribonucleoprotein (RNP) structures. After ... More
Pim serine/threonine kinases regulate the stability of Socs-1 protein.
Authors Chen X Peter; Losman Julie A; Cowan Simone; Donahue Elizabeth; Fay Scott; Vuong Bao Q; Nawijn Martijn C; Capece Danielle; Cohan Victoria L; Rothman Paul;
JournalProc Natl Acad Sci U S A
PubMed ID11854514
Studies of SOCS-1-deficient mice have implicated Socs-1 in the suppression of JAK-STAT (Janus tyrosine kinase-signal transducers and activators of transcription) signaling and T cell development. It has been suggested that the levels of Socs-1 protein may be regulated through the proteasome pathway. Here we show that Socs-1 interacts with members ... More
Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein.
Authors Lindsay Andrew J; Hendrick Alan G; Cantalupo Giuseppina; Senic-Matuglia Francesca; Goud Bruno; Bucci Cecilia; McCaffrey Mary W;
JournalJ Biol Chem
PubMed ID11786538
Rab4 and Rab11 are small GTPases belonging to the Ras superfamily. They both function as regulators along the receptor recycling pathway. We have identified a novel 80-kDa protein that interacts specifically with the GTP-bound conformation of Rab4, and subsequent work has shown that it also interacts strongly with Rab11. We ... More
A major functional difference between the mouse and human ARF tumor suppressor proteins.
Authors Wadhwa Renu; Sugihara Takashi; Hasan Md Kamrul; Taira Kazunari; Reddel Roger R; Kaul Sunil C;
JournalJ Biol Chem
PubMed ID12145279
Suppression of tumorigenesis is considerably more stringent in the human than in the much shorter lived mouse species, and the reasons for this difference are poorly understood. We investigated functional differences in the control of the ARF (alternative reading frame) protein that acts upstream of p53 and is encoded along ... More
Tensin1 and a previously undocumented family member, tensin2, positively regulate cell migration.
Authors Chen Huaiyang; Duncan Ian C; Bozorgchami Hormozd; Lo Su Hao;
JournalProc Natl Acad Sci U S A
PubMed ID11792844
Tensin is a focal adhesion molecule that binds to actin filaments and participates in signaling pathways. In this study, we have characterized a previously undocumented tensin family member, tensin2/KIAA 1075. Human tensin2 cDNA encodes a 1,285-aa sequence that shares extensive homology with tensin1 at its amino- and carboxyl-terminal ends, which ... More
Contribution of Molecular Modeling and Site-directed Mutagenesis to the Identification of Two Structural Residues, Arg-220 and Asp-227, in Aminopeptidase A.
Aminopeptidase A is a zinc metalloenzyme involved in the formation of brain angiotensin III, which exerts a tonic stimulatory action on the central control of blood pressure. Thus, central inhibitors of aminopeptidase A constitute putative central antihypertensive agents. Mutagenic studies have been performed to investigate organization of the aminopeptidase A ... More
The phosphatidylinositol 3-kinase-Akt pathway limits lipopolysaccharide activation of signaling pathways and expression of inflammatory mediators in human monocytic cells.
Authors Guha Mausumee; Mackman Nigel;
JournalJ Biol Chem
PubMed ID12052830
Monocytes and macrophages express cytokines and procoagulant molecules in various inflammatory diseases. In sepsis, lipopolysaccharide (LPS) from Gram-negative bacteria induces tumor necrosis factor-alpha (TNF-alpha) and tissue factor (TF) in monocytic cells via the activation of the transcription factors Egr-1, AP-1, and nuclear factor-kappa B. However, the signaling pathways that negatively ... More
Constitutive activation of G-proteins by polycystin-1 is antagonized by polycystin-2.
Authors Delmas Patrick; Nomura Hideki; Li Xiaogang; Lakkis Montaha; Luo Ying; Segal Yoav; Fernández-Fernández Jose M; Harris Peter; Frischauf Anna-Maria; Brown David A; Zhou Jing;
JournalJ Biol Chem
PubMed ID11786542
Polycystin-1 (PC1), a 4,303-amino acid integral membrane protein of unknown function, interacts with polycystin-2 (PC2), a 968-amino acid alpha-type channel subunit. Mutations in their respective genes cause autosomal dominant polycystic kidney disease. Using a novel heterologous expression system and Ca(2+) and K(+) channels as functional biosensors, we found that full-length ... More
A novel specific role for I kappa B kinase complex-associated protein in cytosolic stress signaling.
We demonstrate here a novel role for the I kappa B kinase complex-associated protein (IKAP) in the regulation of activation of the mammalian stress response via the c-Jun N-terminal kinase (JNK)-signaling pathway. We cloned IKAP as a JNK-associating protein using the Ras recruitment yeast two-hybrid system. IKAP efficiently and specifically ... More
The human MDM2 oncoprotein increases the transcriptional activity and the protein level of the p53 homolog p63.
Authors Calabrò Viola; Mansueto Gelsomina; Parisi Tiziana; Vivo Maria; Calogero Raffaele A; La Mantia Girolama;
JournalJ Biol Chem
PubMed ID11714701
Genetic alteration of the p53 tumor suppressor gene, which monitors DNA damage and operates cell cycle checkpoints, is a major factor in the development of human malignancies. The p53 protein belongs to a family that also includes two structurally related proteins, p63 and p73. Although all three proteins share similar ... More
Targeting and functional role of N-RAP, a nebulin-related LIM protein, during myofibril assembly in cultured chick cardiomyocytes.
Authors Carroll S L; Herrera A H; Horowits R;
JournalJ Cell Sci
PubMed ID11739655
Targeting and functional effects of N-RAP domains were studied by expression as GFP-tagged fusion proteins in cultured embryonic chick cardiomyocytes. GFP-tagged N-RAP was targeted to myofibril precursors, myofibril ends and cell contacts, expression patterns that are similar to endogenous N-RAP. The GFP-tagged N-RAP LIM domain (GFP-N-RAP-LIM) was targeted to the ... More
ATPase activity of the terminase subunit pUL56 of human cytomegalovirus.
Authors Hwang Jae-Seon; Bogner Elke;
JournalJ Biol Chem
PubMed ID11744697
Herpesviral DNA packaging is a complex process resulting in unit-length genomes packed into preformed procapsids. This process is believed to be mediated by two packaging proteins, the terminase subunits. In the case of double-stranded DNA bacteriophages, the translocation of DNA was shown to be an energy-dependent process associated with an ... More
HIV-1 Vpr Induces Apoptosis through Caspase 9 in T Cells and Peripheral Blood Mononuclear Cells.
Authors Muthumani Karuppiah; Hwang Daniel S; Desai Brijal M; Zhang Donghui; Dayes Nathanael; Green Douglas R; Weiner David B;
JournalJ Biol Chem
PubMed ID12095993
Human immunodeficiency virus, type 1 (HIV-1), vpr gene encodes a 14-kDa virion-associated protein, which exhibits significant effects on human cells. One important property of Vpr is its ability to induce apoptosis during infection. Apoptotic induction is likely to play a role in the pathogenesis of AIDS. However, the pathway of ... More
Characterization of a novel radiolabeled peptide selective for a subpopulation of voltage-gated potassium channels in mammalian brain.
Inositol polyphosphate 1-phosphatase is a novel antihypertrophic factor.
Authors Woodcock Elizabeth A; Wang Bing Hui; Arthur Jane F; Lennard Alicia; Matkovich Scot J; Du Xiao-Jun; Brown Joan Heller; Hannan Ross D;
JournalJ Biol Chem
PubMed ID11932254
Activation of G(q)-coupled alpha(1)-adrenergic receptors leads to hypertrophic growth of neonatal rat ventricular cardiomyocytes that is associated with increased expression of hypertrophy-related genes, including atrial natriuretic peptide (ANP) and myosin light chain-2 (MLC), as well as increased ribosome synthesis. The role of inositol phosphates in signaling pathways involved in these ... More
Human mitochondrial ferritin expressed in HeLa cells incorporates iron and affects cellular iron metabolism.
Mitochondrial ferritin (MtF) is a newly identified ferritin encoded by an intronless gene on chromosome 5q23.1. The mature recombinant MtF has a ferroxidase center and binds iron in vitro similarly to H-ferritin. To explore the structural and functional aspects of MtF, we expressed the following forms in HeLa cells: the ... More
Involvement of cell-cell interactions in the rapid stimulation of Cas tyrosine phosphorylation and Src kinase activity by transforming growth factor-beta 1.
Authors Kim Jong-Tak; Joo Choun-Ki;
JournalJ Biol Chem
PubMed ID12065577
Transforming growth factor-beta (TGF-beta) regulates a wide range of physiological and pathological cellular processes, including cell migration, mesenchymal transition, extracellular matrix synthesis, and cell death. Cas (Crk-associated substrate, 130 kDa), an adaptor protein localized at focal adhesions and stress fibers, is also known to have important functions in cell migration ... More
Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator.
Authors Zhang Hui; Peters Kathryn W; Sun Fei; Marino Christopher R; Lang Jochen; Burgoyne Robert D; Frizzell Raymond A;
JournalJ Biol Chem
PubMed ID12039948
The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated chloride channel whose phosphorylation regulates both channel gating and its trafficking at the plasma membrane. Cysteine string proteins (Csps) are J-domain-containing, membrane-associated proteins that have been functionally implicated in regulated exocytosis. Therefore, we evaluated the possibility that Csp is involved ... More
Promoter-specific Activation and Demethylation by MBD2/Demethylase.
MBD2 is the only member of a family of methyl-CpG-binding proteins that has been reported to be both a transcriptional repressor and a DNA demethylase (dMTase). To understand the apparently contradictory function of MBD2/dMTase, we studied the effects of dMTase overexpression on the activity of various in vitro methylated promoters ... More
Structural requirements of anticoagulant protein S for its binding to the complement regulator C4b-binding protein.
Authors Giri Tusar Kanti; Linse Sara; GarcÃa de Frutos Pablo; Yamazaki Tomio; Villoutreix Bruno O; Dahlbäck Björn;
JournalJ Biol Chem
PubMed ID11847209
The vitamin K-dependent anticoagulant protein S binds with high affinity to C4b-binding protein (C4BP), a regulator of complement. Despite the physiological importance of the complex, we have only a patchy view of the C4BP-binding site in protein S. Based on phage display experiments, protein S residues 447-460 were suggested to ... More