'Cyclophilins are enzymes catalyzing the cis-trans isomerization of peptidyl-prolyl bonds and belong to the enzyme class of peptidyl-prolyl cis-trans isomerases (PPIases), which includes two more families (FK506 binding proteins and parvulins). We report the characterization of a novel cyclophilin (Moca-cyp) isolated from Drosophila melanogaster. The single-copy Moca-cyp gene, which is ... More
Gram-negative bacteria-binding protein, a pattern recognition receptor for lipopolysaccharide and beta-1,3-glucan that mediates the signaling for the induction of innate immune genes in Drosophila melanogaster cells.
Authors Kim Y S; Ryu J H; Han S J; Choi K H; Nam K B; Jang I H; Lemaitre B; Brey P T; Lee W J;
JournalJ Biol Chem
PubMed ID10827089
'Pattern recognition receptors, non-clonal immune proteins recognizing common microbial components, are critical for non-self recognition and the subsequent induction of Rel/NF-kappaB-controlled innate immune genes. However, the molecular identities of such receptors are still obscure. Here, we present data showing that Drosophila possesses at least three cDNAs encoding members of the ... More
Drosophila extracellular signal-regulated kinase involves the insulin-mediated proliferation of Schneider cells.
Authors Kwon Hyung-Bae; Kim Sun-Hong; Kim Sung-Eun; Jang In-Hwan; Ahn Yongho; Lee Won-Jae; Choi Kang-Yell;
JournalJ Biol Chem
PubMed ID11834735
'The Drosophila insulin pathway is involved in the control of the proliferation and size of the cell. The stimulation of Schneider cells with human insulin has been observed to activate Drosophila extracellular signal regulated kinase (DERK). However, the role of DERK in the regulation of proliferation is unknown. In this ... More
Human immunodeficiency virus type 1 envelope epitope-specific CD4(+) T lymphocytes in Simian/Human immunodeficiency virus-infected and vaccinated rhesus monkeys detected using a peptide-major histocompatibility complex class II tetramer
'A tetrameric recombinant major histocompatibility complex (MHC) class II-peptide complex was used to quantitate human immunodeficiency virus type 1 (HIV-1) envelope (Env)-specific CD4(+) T cells in vaccinated and in simian/human immunodeficiency virus (SHIV)-infected rhesus monkeys. A rhesus monkey MHC class II DR molecule, Mamu-DR*W201, and an HIV-1 Env peptide (p46) ... More
Lipopolysaccharide-activated kinase, an essential component for the induction of the antimicrobial peptide genes in Drosophila melanogaster cells.
Authors Kim Y S; Han S J; Ryu J H; Choi K H; Hong Y S; Chung Y H; Perrot S; Raibaud A; Brey P T; Lee W J;
JournalJ Biol Chem
PubMed ID10636911
'Eukaryotic organisms use a similar Rel/NF-kappaB signaling cascade for the induction of innate immune genes. In Drosophila, lipopolysaccharide (LPS) signal-induced activation of the Rel/NF-kappaB family transcription factors is an essential step in the transcriptional activation of inducible antimicrobial peptide genes. However, the mechanism by which the LPS-induced signaling pathway proceeds ... More
JIL-1, a chromosomal kinase implicated in regulation of chromatin structure, associates with the male specific lethal (MSL) dosage compensation complex.
Authors Jin Y; Wang Y; Johansen J; Johansen K M;
JournalJ Cell Biol
PubMed ID10831604
JIL-1 is a novel chromosomal kinase that is upregulated almost twofold on the male X chromosome in Drosophila. Here we demonstrate that JIL-1 colocalizes and physically interacts with male specific lethal (MSL) dosage compensation complex proteins. Furthermore, ectopic expression of the MSL complex directed by MSL2 in females causes a ... More
Diagnostic potential of puumala virus nucleocapsid protein expressed inDrosophila melanogaster cells.
AuthorsBrus Sjolander K, Golovljova I, Plyusnin A, Lundkvist A
JournalJ Clin Microbiology
PubMed ID10834996
Puumala virus (PUU) nucleocapsid protein (N) was expressed in insect cells by using the DrosophilaExpression System (DES; Invitrogen BV, Groningen, The Netherlands). Stable transfectants wereestablished by hygromycin B selection and showed continuous expression of the recombinant protein (DES-PUU-N) for at least 5 months. The antigenic property of DES-PUU-N was shown ... More
Expression and purification of functionally active hyaluronan-binding domains from human cartilage link protein, aggrecan and versican: formation of ternary complexes with defined hyaluronan oligosaccharides.
AuthorsSeyfried NT, McVey GF, Almond A, Mahoney DJ, Dudhia J, Day AJ,
JournalJ Biol Chem
PubMed ID15590670
The chondroitin sulfate proteoglycan aggrecan forms link protein-stabilized complexes with hyaluronan (HA), via its N-terminal G1-domain, that provide cartilage with its load bearing properties. Similar aggregates (potentially containing new members of the link protein family), in which other chondroitin sulfate proteoglycans (i.e. versican, brevican, and neurocan) substitute for aggrecan, may ... More
A baculovirus superinfection system: efficient vehicle for gene transfer into drosophila S2 cells.
AuthorsLee DF, Chen CC, Hsu TA, Juang JL
JournalJ Virol
PubMed ID11090187
The baculovirus expression vector system is considered to be a safe, powerful, but cell-lytic heterologous protein expression system in insect cells. We show here that there is a new baculovirus system for efficient gene transfer and expression using the popular and genetically well-understood Drosophila S2 cells. The recombinant baculovirus was ... More
The COOH-terminal domain of the JIL-1 histone H3S10 kinase interacts with histone H3 and is required for correct targeting to chromatin.
AuthorsBao X, Cai W, Deng H, Zhang W, Krencik R, Girton J, Johansen J, Johansen KM,
JournalJ Biol Chem
PubMed ID18819909
The JIL-1 histone H3S10 kinase in Drosophila localizes specifically to euchromatic interband regions of polytene chromosomes and is enriched 2-fold on the male X chromosome. JIL-1 can be divided into four main domains including an NH(2)-terminal domain, two separate kinase domains, and a COOH-terminal domain. Our results demonstrate that the ... More
Functional analysis of mutant and wild-type Drosophila origin recognition complex.
Authors Chesnokov I; Remus D; Botchan M;
JournalProc Natl Acad Sci U S A
PubMed ID11593009
The origin recognition complex (ORC) is the DNA replication initiator protein in eukaryotes. We have reconstituted a functional recombinant Drosophila ORC and compared activities of the wild-type and several mutant ORC variants. Drosophila ORC is an ATPase, and our studies show that the ORC1 subunit is essential for ATP hydrolysis ... More
Zebrafish acetylcholinesterase is encoded by a single gene localized on linkage group 7. Gene structure and polymorphism; molecular forms and expression pattern during development.
Authors Bertrand C; Chatonnet A; Takke C; Yan Y L; Postlethwait J; Toutant J P; Cousin X;
JournalJ Biol Chem
PubMed ID11016933
We cloned and sequenced the acetylcholinesterase gene and cDNA of zebrafish, Danio rerio. We found a single gene (ache) located on linkage group LG7. The relative organization of ache, eng2, and shh genes is conserved between zebrafish and mammals and defines a synteny. Restriction fragment length polymorphism analysis was allowed ... More
Structural requirements for ligand binding by a probable plant vacuolar sorting receptor.
Authors Cao X; Rogers S W; Butler J; Beevers L; Rogers J C;
JournalPlant Cell
PubMed ID10760239
How sorting receptors recognize amino acid determinants on polypeptide ligands and respond to pH changes for ligand binding or release is unknown. The plant vacuolar sorting receptor BP-80 binds polypeptide ligands with a central Asn-Pro-Ile-Arg (NPIR) motif. tBP-80, a soluble form of the receptor lacking transmembrane and cytoplasmic sequences, binds ... More
A novel allelic variant of the human TSG-6 gene encoding an amino acid difference in the CUB module. Chromosomal localization, frequency analysis, modeling, and expression.
Authors Nentwich Hilke A; Mustafa Zehra; Rugg Marilyn S; Marsden Brian D; Cordell Martin R; Mahoney David J; Jenkins Suzanne C; Dowling Barbara; Fries Erik; Milner Caroline M; Loughlin John; Day Anthony J;
JournalJ Biol Chem
PubMed ID11854277
Tumor necrosis factor-stimulated gene-6 (TSG-6) encodes a 35-kDa protein, which is comprised of contiguous Link and CUB modules. TSG-6 protein has been detected in the articular joints of osteoarthritis (OA) patients, with little or no constitutive expression in normal adult tissues. It interacts with components of cartilage matrix (e.g. hyaluronan ... More