Search
Search
查看更多产品信息 BLOCK-iT™ Pol II miR-luc Validated miRNA Control Vector - FAQs (V4935100)
2 个常见问题解答
该载体可用于稳定表达并且能够使用病毒导入。该miR RNAi载体包括内源性 miRNA 的侧翼序列和茎环序列，可指导从较长的Pol lI转录本（pri-miRNA）上切除改造过的miRNA。当存在细胞核时，这些载体可有效使用内源性细胞器加工生成具有敲低作用的序列，这些经过特殊设计的序列与您的目标序列具有100%同源性，可引起靶标切割。此外，茎环序列具有特殊的限制性酶切位点，因此，它能够实现线性化而获得更有效的测序，这对具有发夹结构的标准的shRNA有时是一个挑战。使用该试剂盒可获得超过70%的敲低成功率，轻松追踪表达（可共同表达绿色荧光蛋白），实现同时敲低多个靶标以及组成型或诱导型表达。
These vectors can be used for stable expression and the ability to use viral delivery. These miR RNAi vectors include flanking and loop sequences from an endogenous miRNA which directs the excision of the engineered miRNA from a longer Pol II transcript (pri-miRNA). When present in the nucleus, these vectors efficiently use the endogenous cellular machinery to process knockdown sequences that are specifically designed to have 100% homology to your target of interest and will result in target cleavage. In addition, the loop sequence has a unique restriction site, so that it can be linearized for more efficient sequencing, sometimes a challenge with standard shRNA hairpins. The kits offer over 70% knockdown success, easy expression tracking (with co-cistronic expression of Green Fluorescent Protein), multiple target knockdown, and constitutive or inducible expression.