pLenti6/V5-DEST™ Gateway™ 载体
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Invitrogen™

pLenti6/V5-DEST™ Gateway™ 载体

pLenti6⁄ V5-DEST™ Gateway™ 载体是一种由 Gateway™ -调整的 ViraPower™ 慢病毒表达载体,用于在分裂和非分裂哺乳动物细胞中基于慢病毒的靶基因表达。该载体具有驱动靶基因组成型表达的 CMV了解更多信息
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货号数量
V496106 μg
货号 V49610
价格(CNY)
26,906.85
飞享价
Ends: 31-Dec-2025
32,130.00
共减 5,223.15 (16%)
6 µg
添加至购物车
数量:
6 μg
价格(CNY)
26,906.85
飞享价
Ends: 31-Dec-2025
32,130.00
共减 5,223.15 (16%)
6 µg
添加至购物车
pLenti6⁄ V5-DEST™ Gateway™ 载体是一种由 Gateway™ -调整的 ViraPower™ 慢病毒表达载体,用于在分裂和非分裂哺乳动物细胞中基于慢病毒的靶基因表达。该载体具有驱动靶基因组成型表达的 CMV 启动子和在哺乳动物细胞中稳定选择的杀稻瘟菌素选择标志物。

优点
•分裂和非分裂哺乳动物细胞中基于慢病毒的靶基因表达

主要特点
•灵活且通用的 Gateway™ 重组克隆技术
•使用 CMV 启动子进行组成型高表达
•用于稳定选择的杀稻瘟菌素选择标志物
•用于快速检测的 C 端 V5 标签

试剂盒包括
• pLenti6⁄V5-DEST™ Gateway™ 载体
•One Shot ™ Stbl3™ 化学感受态大肠杆菌 (C7373-03)

相关 SKU
• pLenti6⁄UbC⁄V5-DEST™ Gateway™ 载体 (V49910)
• pLenti4⁄V5-DEST™ Gateway™ 载体 (V49810)
•pLenti6⁄V5 定向 TOPO™ 克隆试剂盒 (K4955-10)
• ViraPower™ 慢病毒定向 TOPO™ 表达试剂盒 (K495000)
•ViraPower ™ 慢病毒 Gateway™ 表达试剂盒 (K4960-00)
•ViraPower ™ HiPerform™ 慢病毒 Gateway™ 表达试剂盒 (K5330-00)

仅供研究使用。不得用于治疗或诊断用途。
仅供科研使用。不可用于诊断程序。
规格
构成或诱导系统组成型
输送类型慢病毒
适用于(应用)病毒表达
产品类型慢病毒表达载体
数量6 μg
选择试剂(真核生物)杀稻瘟菌素
载体pDEST,pLenti
克隆方法Gateway™
产品线Gateway、ViraPower, ViraPower
促进剂CMV
蛋白标记V5 抗原决定簇标签
Unit Size6 µg
内容与储存
在 –20°C 下储存的 ViraPower™ 慢病毒 Gateway™ 载体包括:

pLenti6⁄V5-DEST™:6 µg(40 µL 150 ng/⁄µL 载体 10 mM
Tris-HCl 溶液,1 mM EDTA(pH 值为 8.0))

pLenti6⁄V5-GW⁄lacZ 对照品:10 µg
(20 µL 0.5 µg⁄µL 载体 10 mM
Tris-HCl 溶液,1 mM EDTA(pH 值为 8.0))

One Shot™ Stbl3™ 化学感受态大肠杆菌在 –80°C 下储存

常见问题解答 (FAQ)

可以使用BP Clonase酶和LR Clonase酶替代BP Clonase II 酶LR Clonase II酶进行BP/LR Clonase反应的一步法实验方案吗?

在BP/LR Clonase反应的一步法实验方案中,不建议用BP Clonase酶和LR Clonase酶替代BP Clonase II 酶/LR Clonase II酶,因为这样的重组效率非常低。

有推荐的一步式BP/LR重组实验方案吗?

有的,我们能提供针对BP/LR Clonase反应的一步式实验方案DNA可以在一步反应后被克隆到目的载体中,从而节省了您的时间和金钱。

如果丢失了入门克隆,如何将目的基因从一个Gateway兼容的表达克隆转移到一个新的目的载体?

建议使用一个供体载体进行一次BP反应以获得一个入门克隆。然后将这一入门克隆和目的载体进行一次LR反应以获得新的表达克隆。

我可以单独购买5X LR Clonase缓冲液或5X BP Clonase缓冲液吗?

5X LR Clonase缓冲液或5X BP Clonase缓冲液不作为单独产品出售。它们作为酶试剂盒的一部分进行销售。

是否提供用于在植物内表达的Gateway载体吗?

我们不提供任何用于在植物内表达的Gateway载体。

引用和文献 (4)

引用和文献
Abstract
The membrane anchor R7BP controls the proteolytic stability of the striatal specific RGS protein, RGS9-2.
Authors:Anderson GR, Semenov A, Song JH, Martemyanov KA,
Journal:J Biol Chem
PubMed ID:17158100
'A member of the RGS (regulators of G protein signaling) family, RGS9-2 is a critical regulator of G protein signaling pathways that control locomotion and reward signaling in the brain. RGS9-2 is specifically expressed in striatal neurons where it forms complexes with its newly discovered partner, R7BP (R7 family binding ... More
West Nile virus discriminates between DC-SIGN and DC-SIGNR for cellular attachment and infection.
Authors:Davis CW, Nguyen HY, Hanna SL, Sánchez MD, Doms RW, Pierson TC,
Journal:J Virol
PubMed ID:16415006
'The C-type lectins DC-SIGN and DC-SIGNR bind mannose-rich glycans with high affinity. In vitro, cells expressing these attachment factors efficiently capture, and are infected by, a diverse array of appropriately glycosylated pathogens, including dengue virus. In this study, we investigated whether these lectins could enhance cellular infection by West Nile ... More
Tumorigenesis suppressor Pdcd4 down-regulates mitogen-activated protein kinase kinase kinase kinase 1 expression to suppress colon carcinoma cell invasion.
Authors:Yang HS, Matthews CP, Clair T, Wang Q, Baker AR, Li CC, Tan TH, Colburn NH,
Journal:Mol Cell Biol
PubMed ID:16449643
Programmed cell death 4 (Pdcd4) suppresses neoplastic transformation by inhibiting the activation of c-Jun and consequently AP-1-dependent transcription. We report that Pdcd4 blocks c-Jun activation by inhibiting the expression of mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1)/hematopoietic progenitor kinase 1, a kinase upstream of Jun N-terminal kinase (JNK). cDNA ... More
Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication.
Authors:Bruce JW, Bradley KA, Ahlquist P, Young JA,
Journal:J Virol
PubMed ID:16188999
In order to identify cellular proteins required for early stages of retroviral replication, a high volume screening with mammalian somatic cells was performed. Ten pools of chemically mutagenized Chinese hamster ovary (CHO-K1) cells were challenged with a murine leukemia virus (MLV) vector pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G), ... More