Macelignan inhibits melanosome transfer mediated by protease-activated receptor-2 in keratinocytes.
AuthorsChoi EJ, Kang YG, Kim J, Hwang JK,
JournalBiol Pharm Bull
PubMed ID21532167
'Skin pigmentation is the result of melanosome transfer from melanocytes to keratinocytes. Protease-activated receptor-2 (PAR-2) is a key mediator of melanosome transfer, which occurs as the melanocyte extends its dendrite toward surrounding keratinocytes that take up melanosomes by phagocytosis. We investigated the effects of macelignan isolated from Myristica fragrans HOUTT. ... More
Measurement of growth and viability of cells in culture.
AuthorsPatterson MK
JournalMethods Enzymol
PubMed ID423756
Induction of C-X-C Chemokine Receptor Type 7 (CXCR7) Switches Stromal Cell-derived Factor-1 (SDF-1) Signaling and Phagocytic Activity in Macrophages Linked to Atherosclerosis.
AuthorsMa W, Liu Y, Ellison N, Shen J,
JournalJ Biol Chem
PubMed ID23599431
The discovery of CXCR7 as a new receptor for SDF-1 places many previously described SDF-1 functions attributed to CXCR4 in question, though whether CXCR7 acts as a signaling or ... More
The antimicrobial and phagocytic defense mechanisms of the terrestrial gastropod Lehmannia nyctelia against Escherichia coli and Staphylococcus aureus.
AuthorsLorenson L, DeSantis A, Mannath T, Mirza F, Weeks BS,
JournalMed Sci Monit
PubMed ID20980950
Gastropods are significant vectors of human infectious diseases and are exposed to a wide range of soil parasites and micro-organisms. To defend against infection, we hypothesized that the slug produces both soluble and cellular defense mechanisms. In the gastropod Lehmannia nyctelia, soluble antimicrobial activity was measured by disk diffusion assay ... More
Efficient phagocytosis requires triacylglycerol hydrolysis by adipose triglyceride lipase.
AuthorsChandak PG, Radovic B, Aflaki E, Kolb D, Buchebner M, Fröhlich E, Magnes C, Sinner F, Haemmerle G, Zechner R, Tabas I, Levak-Frank S, Kratky D,
JournalJ Biol Chem
PubMed ID20424161
Macrophage phagocytosis is an essential biological process in host defense and requires large amounts of energy. To date, glucose is believed to represent the prime substrate for ATP production in macrophages. To investigate the relative contribution of free fatty acids (FFAs) in this process, we determined the phagocytosis rates in ... More
Flow cytometric quantitative determination of ingestion by phagocytes needs the distinguishing of overlapping populations of binding and ingesting cells.
AuthorsNuutila J, Lilius EM,
JournalCytometry A
PubMed ID15825183
BACKGROUND: The use of flow cytometry with fluorescently labeled particles provides the means to examine quantitatively the phagocytotic capacity of an individual phagocyte. This report describes an improved flow cytometric method of analysis for kinetic measurement of phagocytosis of fluorescein isothiocyanate (FITC)-labeled zymosan particles by human leukocytes. METHODS: FITC-labeled zymosan ... More
The flow cytometric evaluation of phagocytosis by equine peripheral blood neutrophils and pulmonary alveolar macrophages.
AuthorsRaidal SL, Bailey GD, Love DN
JournalVet J
PubMed ID9805478
Flow cytometry was used to assess the phagocytosis of fluorescent-labelled bacteria by equine peripheral blood neutrophils and pulmonary alveolar macrophages. Cell populations were prepared from venous blood following ammonium chloride lysis and from washed bronchoalveolar lavage derived samples. Discrete clusters of cells, corresponding to different leucocyte groups, were readily identified ... More
Fungal peptide Destruxin A plays a specific role in suppressing the innate immune response in Drosophila melanogaster.
AuthorsPal S, St Leger RJ, Wu LP
JournalJ Biol Chem
PubMed ID17227774
Destruxins are a class of insecticidal, anti-viral, and phytotoxic cyclic depsipeptides that are also studied for their toxicity to cancer cells. They are produced by various fungi, and a direct relationship has been established between Destruxin production and the virulence of the entomopathogen Metarhizium anisopliae. Aside from opening calcium channels, ... More
The protease-activated receptor-2 upregulates keratinocyte phagocytosis.
AuthorsSharlow ER, Paine CS, Babiarz L, Eisinger M, Shapiro S, Seiberg M
JournalJ Cell Sci
PubMed ID10934047
The protease-activated receptor-2 (PAR-2) belongs to the family of seven transmembrane domain receptors, which are activated by the specific enzymatic cleavage of their extracellular amino termini. Synthetic peptides corresponding to the tethered ligand domain (SLIGRL in mouse, SLIGKV in human) can activate PAR-2 without the need for receptor cleavage. PAR-2 ... More
A new simple fluorometric assay for phagocytosis.
AuthorsOda T, Maeda H
JournalJ Immunol Methods
PubMed ID3514764
A highly sensitive, but simple and quantitative, fluorometric assay method for phagocytosis by cells such as macrophages and polymorphonuclear leukocytes was developed by utilizing fluorescent particles. Escherichia coli, Serratia marcescens, yeast, and latex particles were conjugated with fluorescein isothiocyanate and used as fluorescent particles. The assay procedure requires phagocytic cells, ... More
A rapid and simple microfluorometric phagocytosis assay.
AuthorsWan CP, Park CS, Lau BH
JournalJ Immunol Methods
PubMed ID8509646
A microfluorometric method for phagocytosis study has been developed using fluorescein conjugated Escherichia coli K-12 particles. This technique is based on the uptake of fluorescent particles and quenching of extracellular fluorescence at the end of the assay. A murine macrophage cell line, J774, was used as a phagocyte model. The ... More
Phagocytosis of Escherichia coli by insect hemocytes requires both activation of the Ras/mitogen-activated protein kinase signal transduction pathway for attachment and beta3 integrin for internalization.
AuthorsFoukas LC, Katsoulas HL, Paraskevopoulou N, Metheniti A, Lambropoulou M, Marmaras VJ
JournalJ Biol Chem
PubMed ID9614082
Insect hemocytes in response to lipopolysaccharide (LPS) of Gram-negative bacteria facilitate binding and internalization of either cell-associated or cell-free LPS (Charalambidis, N. D., Foukas L. C., and Marmaras V. J. (1996) Eur. J. Biochem. 236, 200-206). An early event in LPS signaling in hemocytes involves protein tyrosine phosphorylation (Charalambidis N. ... More
Neuroimmune Regulation of JC Virus by Intracellular and Extracellular Agnoprotein.
Authors
JournalJ Neuroimmune Pharmacol
PubMed ID29159704
Normal human monocytes exposed to glioma cells acquire myeloid-derived suppressor cell-like properties.
Authors
JournalNeuro Oncol
PubMed ID20308313
IGF1 Shapes Macrophage Activation in Response to Immunometabolic Challenge.
Authors
JournalCell Rep
PubMed ID28402847
Functional Analysis of Genetic Variations in Surfactant Protein D in Mycobacterial Infection and Their Association With Tuberculosis.
Authors
JournalFront Immunol
PubMed ID30013576
Chlamydia psittaci PmpD-N Exacerbated Chicken Macrophage Function by Triggering Th2 Polarization and the TLR2/MyD88/NF-κB Signaling Pathway.
Authors
JournalInt J Mol Sci
PubMed ID32183481
Chronic stress primes innate immune responses in mice and humans.
Authors
JournalCell Rep
PubMed ID34496250
Biologically active polymers from spontaneous carotenoid oxidation: a new frontier in carotenoid activity.
Authors
JournalPLoS One
PubMed ID25360750
Daily variation in macrophage phagocytosis is clock-independent and dispensable for cytokine production.