Requirement of helix 1 and the AF-2 domain of the thyroid hormone receptor for coactivation by PGC-1.
Authors Wu Yifei; Delerive Philippe; Chin William W; Burris Thomas P;
JournalJ Biol Chem
PubMed ID11751919
'Although PGC-1 (peroxisome proliferator-activated receptor-gamma coactivator-1) has been previously shown to enhance thyroid hormone receptor (TR)/retinoid X receptor-mediated ucp-1 gene expression in a ligand-induced manner in rat fibroblast cells, the precise mechanism of PGC-1 modulation of TR function has yet to be determined. In this study, we show that PGC-1 ... More
Cloning and characterization of a cell surface receptor for xenotropic and polytropic murine leukemia viruses [see comments]
AuthorsTailor CS, Nouri A, Lee CG, Kozak C, Kabat D
JournalProc Natl Acad Sci U S A
PubMed ID9927670
'Xenotropic and polytropic murine leukemia viruses (X-MLVs and P-MLVs) cross-interfere to various extents in non-mouse species and in wild Asian mice, suggesting that they might use a common receptor for infection. Consistent with this hypothesis, the susceptibility of some wild mice to X-MLVs has been mapped to the P-MLV receptor ... More
NF-Y is associated with the histone acetyltransferases GCN5 and P/CAF.
AuthorsCurrie RA
JournalJ Biol Chem
PubMed ID9430679
'The ubiquitous transcription factor, NF-Y, plays a pivotal role in the cell cycle regulation of the mammalian cyclin A, cdc25C, and cdc2 genes, in the S-phase activation of the ribonucleotide reductase R2 gene, in addition to its critical role as a key proximal promoter factor in the transcriptional regulation of ... More
Upstream stimulatory factors binding to an E box motif in the R region of the bovine leukemia virus long terminal repeat stimulates viral gene expression.
Authors Calomme Claire; Nguyen Thi Lien-Anh; de Launoit Yvan; Kiermer Veronique; Droogmans Louis; Burny Arsene; Van Lint Carine;
JournalJ Biol Chem
PubMed ID11741930
'The bovine leukemia virus (BLV) promoter is located in its 5''-long terminal repeat and is composed of the U3, R, and U5 regions. BLV transcription is regulated by cis-acting elements located in the U3 region, including three 21-bp enhancers required for transactivation of the BLV promoter by the virus-encoded transactivator ... More
Visualization of agonist-induced sequestration and down-regulation of a green fluorescent protein-tagged beta2-adrenergic receptor.
AuthorsKallal L, Gagnon AW, Penn RB, Benovic JL
JournalJ Biol Chem
PubMed ID9417083
'To date, the visualization of beta2-adrenergic receptor (beta2AR) trafficking has been largely limited to immunocytochemical analyses of acute internalization events of epitope-tagged receptors in various transfection systems. The development of a beta2AR conjugated with green fluorescent protein (beta2AR-GFP) provides the opportunity for a more extensive optical analysis of beta2AR sequestration, ... More
Ligand binding to macrophage scavenger receptor-A induces urokinase- type plasminogen activator expression by a protein kinase-dependent signaling pathway.
AuthorsHsu HY, Hajjar DP, Khan KM, Falcone DJ
JournalJ Biol Chem
PubMed ID9422792
'Macrophage scavenger receptor-type A (MSR-A) has been implicated in the transmission of cell signals and the regulation of diverse cellular functions (Falcone, D. J., and Ferenc, M. J. (1988) J. Cell. Physiol. 135, 387-396; Falcone, D. J., McCaffrey, T. A., and Vergilio, J. A. (1991) J. Biol. Chem. 266, 22726-22732; ... More
Intercellular calcium signaling via gap junction in connexin-43- transfected cells [published erratum appears in J Biol Chem 1998 Aug 28;273(35):22856]
AuthorsToyofuku T, Yabuki M, Otsu K, Kuzuya T, Hori M, Tada M
JournalJ Biol Chem
PubMed ID9430691
'In excitable cells, intracellular Ca2+ is released via the ryanodine receptor from the intracellular Ca2+ storing structure, the sarcoplasmic reticulum. To determine whether this released Ca2+ propagates through gap junctions to neighboring cells and thereby constitutes a long range signaling network, we developed a cell system in which cells expressing ... More
Urokinase-type plasminogen activator receptor (CD87) is a ligand for integrins and mediates cell-cell interaction.
Authors Tarui T; Mazar A P; Cines D B; Takada Y;
JournalJ Biol Chem
PubMed ID11053440
'Binding of urokinase-type plasminogen activator (uPA) to its receptor (uPAR/CD87) regulates cellular adhesion, migration, and tumor cell invasion. However, it is unclear how glycosyl phosphatidylinositol-anchored uPAR, which lacks a transmembrane structure, mediates signal transduction. It has been proposed that uPAR forms cis-interactions with integrins as an associated protein and thereby ... More
The ebola virus VP35 protein functions as a type I IFN antagonist
AuthorsBasler CF, Wang X, Muhlberger E, Volchkov V, Paragas J, Klenk HD, Garcia-Sastre A, Palese P
JournalProc Natl Acad Sci U S A
PubMed ID11027311
'An assay has been developed that allows the identification of molecules that function as type I IFN antagonists. Using this assay, we have identified an Ebola virus-encoded inhibitor of the type I IFN response, the Ebola virus VP35 protein. The assay relies on the properties of an influenza virus mutant, ... More
The Role of Constant Region Carbohydrate in the Assembly and Secretion of Human IgD and IgA1.
Authors Gala Francoise A; Morrison Sherie L;
JournalJ Biol Chem
PubMed ID12023968
'Immunoglobulins are glycoproteins, containing N- linked carbohydrates in the heavy chain constant regions of all isotypes and O-linked carbohydrates in the hinge regions of human IgA1 and IgD. A previous study showed that IgD synthesized in the presence of tunicamycin and lacking the three N-linked glycans on the heavy chain ... More
Molecular cloning and expression of a novel human trans-Golgi network glycoprotein, TGN51, that contains multiple tyrosine-containing motifs.
AuthorsKain R, Angata K, Kerjaschki D, Fukuda M
JournalJ Biol Chem
PubMed ID9422759
'Previously, it has been shown that glycoproteins with approximately 130- kDa molecular mass react with antisera from patients with renal vasculitis (Kain, R., Matsui, K., Exner, M., Binder, S., Schaffner, G., Sommer, E. M., and Kerjaschki, D. (1995) J. Exp. Med. 181, 585-597). To search for a molecule that reacts ... More
A 70 amino acid region within the semaphorin domain activates specific cellular response of semaphorin family members.
AuthorsKoppel AM, Feiner L, Kobayashi H, Raper JA
JournalNeuron
PubMed ID9331346
'The semaphorin family contains secreted and transmembrane signaling proteins that function in the nervous, immune, and cardiovascular systems. Chick collapsin-1 is a repellent for specific growth cones. Two other secreted members of the semaphorin family, collapsin-2 and - 3, are structurally similar to collapsin-1 but have different biological activities. Semaphorins ... More
Developmentally regulated expression of the novel cancer anti-apoptosis gene survivin in human and mouse differentiation.
'Inhibitors of programmed cell death (apoptosis) may regulate tissue differentiation and aberrantly promote cell survival in neoplasia. A novel apoptosis inhibitor of the IAP gene family, designated survivin, was recently found in all of the most common human cancers but not in normal, terminally differentiated adult tissues. The expression of ... More
Secreted chick semaphorins bind recombinant neuropilin with similar affinities but bind different subsets of neurons in situ.
AuthorsFeiner L, Koppel AM, Kobayashi H, Raper JA
JournalNeuron
PubMed ID9331347
'Collapsin-1, a member of the semaphorin family, activates receptors on specific growth cones, thereby inhibiting their motility. Neuropilin, a previously cloned transmembrane protein, has recently been identified as a candidate receptor for collapsin-1. We have completed the cloning of chick collapsin-3 and -5 and show that collapsin-1, -2, -3, and ... More
The structural basis for the specificity of retinoid-X receptor-selective agonists: new insights into the role of helix H12.
Authors Love James D; Gooch John T; Benko Szilvia; Li Chuan; Nagy Laszlo; Chatterjee V Krishna K; Evans Ronald M; Schwabe John W R;
JournalJ Biol Chem
PubMed ID11782480
Ligands that specifically target retinoid-X receptors (RXRs) are emerging as potentially powerful therapies for cancer, diabetes, and the lowering of circulatory cholesterol. To date, RXR has only been crystallized in the absence of ligand or with the promiscuous ligand 9-cis retinoic acid, which also activates retinoic acid receptors. Here we ... More
Neuregulin and erbB receptors play a critical role in neuronal migration [published erratum appears in Neuron 1997 Dec;19(6):1349]
AuthorsRio C, Rieff HI, Qi P, Khurana TS, Corfas G
JournalNeuron
PubMed ID9247262
The migration of neuronal precursors along radial glial fibers is a critical step in the formation of the nervous system. In this report, we show that neuregulin-erbB receptor signaling plays a crucial role in the migration of cerebellar granule cells along radial glial fibers. Granule cells express neuregulin (NRG), and ... More
Tyrosine kinase-dependent suppression of a potassium channel by the G protein-coupled m1 muscarinic acetylcholine receptor.
AuthorsHuang XY, Morielli AD, Peralta EG
JournalCell
PubMed ID8261514
Neurotransmitter receptors alter membrane excitability and synaptic efficacy by generating intracellular signals that ultimately change the properties of ion channels. Through expression studies in Xenopus oocytes and mammalian cells, we found that the G protein-coupled m1 muscarinic acetylcholine receptor potently suppresses a cloned delayed rectifier K+ channel through a pathway ... More
A new dexamethasone-induced gene of the leucine zipper family protects T lymphocytes from TCR/CD3-activated cell death.
AuthorsD'Adamio F, Zollo O, Moraca R, Ayroldi E, Bruscoli S, Bartoli A, Cannarile L, Migliorati G, Riccardi C
JournalImmunity
PubMed ID9430225
By comparing mRNA species expressed in dexamethasone (DEX)-treated and untreated murine thymocytes, we have identified a gene, glucocorticoid- induced leucine zipper (GILZ), encoding a new member of the leucine zipper family. GILZ was found expressed in normal lymphocytes from thymus, spleen, and lymph nodes, whereas low or no expression was ... More
Direct, activating interaction between glycogen synthase kinase-3beta and p53 after DNA damage.
Authors Watcharasit Piyajit; Bijur Gautam N; Zmijewski Jaroslaw W; Song Ling; Zmijewska Anna; Chen Xinbin; Johnson Gail V W; Jope Richard S;
JournalProc Natl Acad Sci U S A
PubMed ID12048243
Glycogen synthase kinase-3beta (GSK3beta) is a central figure in Wnt signaling, in which its activity is controlled by regulatory binding proteins. Here we show that binding proteins outside the Wnt pathway also control the activity of GSK3beta. DNA damage induced by camptothecin, which activates the tumor suppressor p53, was found ... More
Neuropilin-1 is expressed by endothelial and tumor cells as an isoform- specific receptor for vascular endothelial growth factor.
AuthorsSoker S, Takashima S, Miao HQ, Neufeld G, Klagsbrun M
JournalCell
PubMed ID9529250
Vascular endothelial growth factor (VEGF), a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt- 1. We now describe the purification and the expression cloning from tumor cells of a third VEGF receptor, one that binds VEGF165 but not VEGF121. This isoform-specific VEGF receptor (VEGF165R) is ... More
Expression cloning of LDLB, a gene essential for normal Golgi function and assembly of the ldlCp complex.
AuthorsChatterton JE, Hirsch D, Schwartz JJ, Bickel PE, Rosenberg RD, Lodish HF, Krieger M
JournalProc Natl Acad Sci U S A
PubMed ID9927668
The Chinese hamster ovary (CHO) cell mutants ldlC and ldlB, which exhibit almost identical phenotypes, define two genes required for multiple steps in the normal medial and trans Golgi-associated processing of glycoconjugates. The LDLC gene encodes ldlCp, an approximately 80-kDa protein, which in wild-type, but not ldlB, cells associates reversibly ... More
Human single-chain Fv immunoconjugates targeted to a melanoma- associated chondroitin sulfate proteoglycan mediate specific lysis of human melanoma cells by natural killer cells and complement.
AuthorsWang B, Chen YB, Ayalon O, Bender J, Garen A
JournalProc Natl Acad Sci U S A
PubMed ID9990075
Two antimelanoma immunoconjugates containing a human single-chain Fv (scFv) targeting domain conjugated to the Fc effector domain of human IgG1 were synthesized as secreted two-chain molecules in Chinese hamster ovary and Drosophila S2 cells, and purified by affinity chromatography on protein A. The scFv targeting domains originally were isolated as ... More
Minors held by majors: the H13 minor histocompatibility locus defined as a peptide/MHC class I complex.
AuthorsMendoza LM, Paz P, Zuberi A, Christianson G, Roopenian D, Shastri N
JournalImmunity
PubMed ID9354467
The products of minor histocompatibility (H) loci are serious barriers to tissue transplantation even among major histocompatibility complex (MHC) identical individuals, frequently causing chronic graft rejection and graft versus host disease. Over 50 minor H loci map to mouse autosomal chromosomes but none are known at the molecular level. By ... More
Identification of functionally important residues of human thrombopoietin.
AuthorsPark H, Park SS, Jin EH, Song JS, Ryu SE, Yu MH, Hong HJ
JournalJ Biol Chem
PubMed ID9417073
Thrombopoietin (TPO) is a megakaryocyte growth and differentiation factor. It consists of a characteristic two domain structure. The amino- terminal domain of TPO has a sequence homology with erythropoietin and is required for the binding and activation of its receptor c-Mpl. To determine the functionally important regions interacting with its ... More
Essential requirement of cytosolic phospholipase A2 for activation of the phagocyte NADPH oxidase.
AuthorsDana R, Leto TL, Malech HL, Levy R
JournalJ Biol Chem
PubMed ID9417101
Arachidonic acid (AA) can trigger activation of the phagocyte NADPH oxidase in a cell-free assay. However, a role for AA in activation of the oxidase in intact cells has not been established, nor has the AA generating enzyme critical to this process been identified. The human myeloid cell line PLB-985 ... More
The alpha chain of the T cell antigen receptor is degraded in the cytosol.
AuthorsHuppa JB, Ploegh HL
JournalImmunity
PubMed ID9252124
To reach the cell surface, the T cell receptor for antigen (TCR)-CD3 complex must assemble in the endoplasmic reticulum (ER), where single subunits are retained and degraded. However, the exact location of breakdown and the mechanism and proteases involved in destruction of free subunits have remained elusive. We show that ... More
Influence of the cytosolic carboxyl termini of human B1 and B2 kinin receptors on receptor sequestration, ligand internalization, and signal transduction.
AuthorsFaussner A, Proud D, Towns M, Bathon JM
JournalJ Biol Chem
PubMed ID9446564
To determine the role of the cytoplasmic carboxyl termini of human B1 and B2 kinin receptors (B1KR and B2KR, respectively) in the internalization of their respective ligands, des-Arg10-kallidin and bradykinin (BK), both wild type receptors, as well as truncated B2KRs, a mutated B2KR, and chimeric receptors were stably expressed in ... More
EBF and E47 collaborate to induce expression of the endogenous immunoglobulin surrogate light chain genes.
AuthorsSigvardsson M, O'Riordan M, Grosschedl R
JournalImmunity
PubMed ID9252117
Early B cell factor (EBF) and E47 participate in the transcriptional control of early B lymphocyte differentiation. With the aim of identifying genetic targets for these transcription factors, we stably transfected cDNAs encoding EBF or a covalent homodimer of E47, individually or together, into immature hematopoietic Ba/F3 cells, which lack ... More
CCR5 levels and expression pattern correlate with infectability by macrophage-tropic HIV-1, in vitro.
AuthorsWu L, Paxton WA, Kassam N, Ruffing N, Rottman JB, Sullivan N, Choe H, Sodroski J, Newman W, Koup RA, Mackay CR
JournalJ Exp Med
PubMed ID9151905
Chemokine receptors serve as coreceptors for HIV entry into CD4+ cells. Their expression is thought to determine the tropism of viral strains for different cell types, and also to influence susceptibility to infection and rates of disease progression. Of the chemokine receptors, CCR5 is the most important for viral transmission, ... More
Mutations in the second cytoplasmic loop of the rat parathyroid hormone (PTH)/PTH-related protein receptor result in selective loss of PTH- stimulated phospholipase C activity.
AuthorsIida-Klein A, Guo J, Takemura M, Drake MT, Potts JT Jr, Abou-Samra A, Bringhurst FR, Segre GV
JournalJ Biol Chem
PubMed ID9054374
To define the structural requirements of the parathyroid hormone (PTH)/PTH-related protein (PTHrP) receptor necessary for activation of phospholipase C (PLC), receptors with random mutations in their second cytoplasmic loop were synthesized, and their properties were assessed. A mutant in which the wild type (WT) rat PTH/PTHrP receptor sequence EKKY (amino ... More
Functional domains of the nuclear receptor hepatocyte nuclear factor 4.
AuthorsHadzopoulou-Cladaras M, Kistanova E, Evagelopoulou C, Zeng S, Cladaras C, Ladias JA
JournalJ Biol Chem
PubMed ID8995295
The hepatocyte nuclear factor 4 (HNF-4) is a member of the nuclear receptor superfamily and participates in the regulation of several genes involved in diverse metabolic pathways and developmental processes. To date, the functional domains of this nuclear receptor have not been identified, and it is not known whether its ... More
Aspirin-triggered 15-epi-lipoxin A4 (LXA4) and LXA4 stable analogues are potent inhibitors of acute inflammation: evidence for anti- inflammatory receptors.
Lipoxins are bioactive eicosanoids that are immunomodulators. In human myeloid cells, lipoxin (LX) A4 actions are mediated by interaction with a G protein-coupled receptor. To explore functions of LXA4 and aspirin- triggered 5(S),6(R),15(R)-trihydroxy-7,9,13-trans-11-cis- eicosatetraenoic acid (15-epi-LXA4) in vivo, we cloned and characterized a mouse LXA4 receptor (LXA4R). When expressed in ... More
The heterogeneous nuclear ribonucleoproteins I and K interact with a subset of the ro ribonucleoprotein-associated Y RNAs in vitro and in vivo.
The hY RNAs are a group of four small cytoplasmic RNAs of unknown function that are stably associated with at least two proteins, Ro60 and La, to form Ro ribonucleoprotein complexes. Here we show that the heterogeneous nuclear ribonucleoproteins (hnRNP) I and K are able to associate with a subset ... More
Discrimination between RelA and RelB transcriptional regulation by a dominant negative mutant of IkappaBalpha.
AuthorsFerreira V, Tarantino N, Korner M
JournalJ Biol Chem
PubMed ID9417120
RelA and RelB belong to the nuclear factor-kappaB (NF-kappaB-Rel) transcription factor family. Both proteins are structurally and functionally related, but their intracellular and tissue distributions are different. In resting cells, RelB is found mostly in the nucleus, whereas RelA is sequestered in the cytosol by protein inhibitors, among which IkappaBalpha ... More
The p42/p44 mitogen-activated protein kinase cascade is determinant in mediating activation of the Na+/H+ exchanger (NHE1 isoform) in response to growth factors.
AuthorsBianchini L, L'Allemain G, Pouyssegur J
JournalJ Biol Chem
PubMed ID8995258
The ubiquitously expressed Na+/H+ exchanger NHE1 is the target of multiple signaling pathways, including those activated by tyrosine kinase receptors, G protein-coupled receptors, and integrins. The intracellular pathways leading to activation of NHE1 are poorly understood. To gain more insight into these activation pathways, we examined the role of mitogen-activated ... More
Identification of estrogen receptor beta2, a functional variant of estrogen receptor beta expressed in normal rat tissues.
AuthorsPetersen DN, Tkalcevic GT, Koza-Taylor PH, Turi TG, Brown TA
JournalEndocrinology
PubMed ID9492041
The effects of estrogen and estrogen agonists can be mediated by estrogen receptor alpha (ER alpha) and estrogen receptor beta (ER beta). We now report the identification and initial characterization of several novel isoforms of rat ER beta messenger RNA (mRNA). The most abundant of these mRNA variants we have ... More
Modulation of cardiac Na+ channels expressed in a mammalian cell line and in ventricular myocytes by protein kinase C.
AuthorsQu Y, Rogers J, Tanada T, Scheuer T, Catterall WA
JournalProc Natl Acad Sci U S A
PubMed ID8159741
Cardiac rH1 Na+ channel alpha subunits were expressed in cells of the Chinese hamster lung 1610 cell line by transfection, and a stable cell line expressing cardiac Na+ channels (SNa-rH1) was isolated. Mean Na+ currents of 2.2 +/- 1.0 nA were recorded, which corresponds to a cell surface density of ... More
Identification, tissue expression, and functional characterization of Otx3, a novel member of the Otx family.
Transcription factors containing a homeodomain play an important role in the organogenesis of vertebrates. We have isolated a novel homeodomain transcription factor, Otx3, which is structurally and functionally related to Otx1 and Otx2, transcription factors that are critical in brain morphogenesis. Mouse Otx3 is a protein composed of 376 amino ... More
Multidrug-resistant human sarcoma cells with a mutant P-glycoprotein, altered phenotype, and resistance to cyclosporins.
A variant of the multidrug-resistant human sarcoma cell line Dx5 was derived by co-selection with doxorubicin and the cyclosporin D analogue PSC 833, a potent inhibitor of the multidrug transporter P-glycoprotein. The variant DxP cells manifest an altered phenotype compared with Dx5, with decreased cross-resistance to Vinca alkaloids and no ... More
Peg3/Pw1 is involved in p53-mediated cell death pathway in brain ischemia/hypoxia.
Emerging evidence has shown that tumor suppressor p53 expression is enhanced in response to brain ischemia/hypoxia and that p53 plays a critical role in the cell death pathway in such an acute neurological insult. However the mechanism remains unclear. Recently it was reported that Peg3/Pw1, originally identified as a paternally ... More
Conserved Kv4 N-terminal domain critical for effects of Kv channel-interacting protein 2.2 on channel expression and gating.
Association of Kv channel-interacting proteins (KChIPs) with Kv4 channels leads to modulation of these A-type potassium channels (An, W. F., Bowlby, M. R., Betty, M., Cao, J., Ling, H. P., Mendoza, G., Hinson, J. W., Mattsson, K. I., Strassle, B. W., Trimmer, J. S., and Rhodes, K. J. (2000) Nature ... More
Herstatin, an autoinhibitor of the human epidermal growth factor receptor 2 tyrosine kinase, modulates epidermal growth factor signaling pathways resulting in growth arrest.
Authors Justman Quincey A; Clinton Gail M;
JournalJ Biol Chem
PubMed ID11934884
Herstatin is an autoinhibitor of the ErbB family consisting of subdomains I and II of the human epidermal growth factor receptor 2 (ErbB-2) extracellular domain and a novel C-terminal domain encoded by an intron. Herstatin binds to human epidermal growth factor receptor 2 and to the epidermal growth factor receptor ... More
V490M, a Common Mutation in 3-Phosphoglycerate Dehydrogenase Deficiency, Causes Enzyme Deficiency by Decreasing the Yield of Mature Enzyme.
Authors Pind Steven; Slominski Elzbieta; Mauthe Jill; Pearlman Kayla; Swoboda Kathryn J.; Wilkins John A.; Sauder Patricia; Natowicz Marvin R.;
JournalJ Biol Chem
PubMed ID11751922
A deficiency of 3-phosphoglycerate dehydrogenase (PHGDH) is a disorder of serine biosynthesis identified in children with congenital microcephaly, seizures, and severe psychomotor retardation. We report here the identification of the 1468G→A (V490M) mutation of this gene in two siblings of an Ashkenazi Jewish family, providing further evidence that the V490M ... More
Cloning and characterization of human Siglec-11. A recently evolved signaling that can interact with SHP-1 and SHP-2 and is expressed by tissue macrophages, including brain microglia.
Authors Angata Takashi; Kerr Sheena C; Greaves David R; Varki Nissi M; Crocker Paul R; Varki Ajit;
JournalJ Biol Chem
PubMed ID11986327
Siglecs are sialic acid-recognizing animal lectins of the immunoglobulin superfamily. We have cloned and characterized a novel human molecule, Siglec-11, that belongs to the subgroup of CD33/Siglec-3-related Siglecs. As with others in this subgroup, the cytosolic domain of Siglec-11 is phosphorylated at tyrosine residue(s) upon pervanadate treatment of cells and ... More
Differential use of myristoyl groups on neuronal calcium sensor proteins as a determinant of spatio-temporal aspects of Ca2+ signal transduction.
Authors O'Callaghan Dermott W; Ivings Lenka; Weiss Jamie L; Ashby Michael C; Tepikin Alexei V; Burgoyne Robert D;
JournalJ Biol Chem
PubMed ID11836243
The localizations of three members of the neuronal calcium sensor (NCS) family were studied in HeLa cells. Using hippocalcin-EYFP and NCS-1-ECFP, it was found that their localization differed dramatically in resting cells. NCS-1 had a distinct predominantly perinuclear localization (similar to trans-Golgi markers), whereas hippocalcin was present diffusely throughout the ... More