'The glucocorticoid and mineralocorticoid receptors (GR and MR) share considerable structural and functional homology and bind as homodimers to hormone-response elements. We have shown previously that MR and GR can form heterodimers that inhibit transcription from a glucocorticoid (GC)-responsive gene and that this inhibition was mediated by the N-terminal domain ... More
The transcription factors GATA4 and dHAND physically interact to synergistically activate cardiac gene expression through a p300-dependent mechanism.
Authors Dai Yan-Shan; Cserjesi Peter; Markham Bruce E; Molkentin Jeffery D;
JournalJ Biol Chem
PubMed ID11994297
'An intricate array of heterogeneous transcription factors participate in programming tissue-specific gene expression through combinatorial interactions that are unique to a given cell-type. The zinc finger-containing transcription factor GATA4, which is widely expressed in mesodermal and endodermal derived tissues, is thought to regulate cardiac myocyte-specific gene expression through combinatorial interactions ... More
Identification of a cryptic nucleolar-localization signal in MDM2.
AuthorsLohrum MA, Ashcroft M, Kubbutat MH, Vousden KH
JournalNature Cell Biology
PubMed ID10707090
N/A
Direct, activating interaction between glycogen synthase kinase-3beta and p53 after DNA damage.
Authors Watcharasit Piyajit; Bijur Gautam N; Zmijewski Jaroslaw W; Song Ling; Zmijewska Anna; Chen Xinbin; Johnson Gail V W; Jope Richard S;
JournalProc Natl Acad Sci U S A
PubMed ID12048243
Glycogen synthase kinase-3beta (GSK3beta) is a central figure in Wnt signaling, in which its activity is controlled by regulatory binding proteins. Here we show that binding proteins outside the Wnt pathway also control the activity of GSK3beta. DNA damage induced by camptothecin, which activates the tumor suppressor p53, was found ... More
The organellular chloride channel protein CLIC4/mtCLIC translocates to the nucleus in response to cellular stress and accelerates apoptosis.
AuthorsSuh KS, Mutoh M, Nagashima K, Fernandez-Salas E, Edwards LE, Hayes DD, Crutchley JM, Marin KG, Dumont RA, Levy JM, Cheng C, Garfield S, Yuspa SH,
JournalJ Biol Chem
PubMed ID14610078
CLIC4/mtCLIC, a chloride intracellular channel protein, localizes to the mitochondria and cytoplasm of keratinocytes and participates in the apoptotic response to stress. We now show that multiple stress inducers cause the translocation of cytoplasmic CLIC4 to the nucleus. Immunogold electron microscopy and confocal analyses indicate that nuclear CLIC4 is detected ... More
Intracellular localization and activity state of tissue transglutaminase differentially impacts cell death.
AuthorsMilakovic T, Tucholski J, McCoy E, Johnson GV,
JournalJ Biol Chem
PubMed ID14670969
Tissue transglutaminase (tTG) is a unique member of the transglutaminase family as it is both a transamidating enzyme and a GTPase. In the cell tTG is mostly cytosolic, however it is also found in the nucleus and associated with the plasma membrane. tTG can be proapoptotic, however anti-apoptotic activities of ... More
Authors McCampbell A; Taye A A; Whitty L; Penney E; Steffan J S; Fischbeck K H;
JournalProc Natl Acad Sci U S A
PubMed ID11742087
Polyglutamine diseases include at least nine neurodegenerative disorders, each caused by a CAG repeat expansion in a different gene. Accumulation of mutant polyglutamine-containing proteins occurs in patients, and evidence from cell culture and animal experiments suggests the nucleus as a site of pathogenesis. To understand the consequences of nuclear accumulation, ... More
Peptide blockade of HIFalpha degradation modulates cellular metabolism and angiogenesis.
Authors Willam Carsten; Masson Norma; Tian Ya-Min; Mahmood S Aleema; Wilson Michael I; Bicknell Roy; Eckardt Kai-Uwe; Maxwell Patrick H; Ratcliffe Peter J; Pugh Christopher W;
JournalProc Natl Acad Sci U S A
PubMed ID12149454
Hypoxia-inducible factor-1 (HIF) is a transcription factor central to oxygen homeostasis. It is regulated via its alpha isoforms. In normoxia they are ubiquitinated by the von Hippel-Lindau E3 ligase complex and destroyed by the proteasome, thereby preventing the formation of an active transcriptional complex. Oxygen-dependent enzymatic hydroxylation of either of ... More