The large conductance potassium channel beta-subunit can interact with and modulate the functional properties of a calcium-activated chloride channel, CLCA1.
Authors Greenwood Iain A; Miller Lisa J; Ohya Susumu; Horowitz Burton;
JournalJ Biol Chem
PubMed ID11994272
'We have recently compared the biophysical and pharmacological properties of native Ca(2+)-activated Cl(-) currents in murine portal vein with mCLCA1 channels cloned from murine portal vein myocytes (Britton, F. C., Ohya, S., Horowitz, B., and Greenwood, I. A. (2002) J. Physiol. (Lond.) 539, 107-117). These channels shared a similar relative ... More
Enhanced production and oligomerization of the 42-residue amyloid beta- protein by Chinese hamster ovary cells stably expressing mutant presenilins.
AuthorsXia W, Zhang J, Kholodenko D, Citron M, Podlisny MB, Teplow DB, Haass C, Seubert P, Koo EH, Selkoe DJ
JournalJ Biol Chem
PubMed ID9065468
'Mutations in the presenilin 1 (PS1) and presenilin 2 (PS2) genes cause the most common and aggressive form of early onset familial Alzheimer''s disease. To elucidate their pathogenic mechanism, wild-type (wt) or mutant (M146L, C410Y) PS1 and wt or mutant (M239V) PS2 genes were stably transfected into Chinese hamster ovary ... More
The beta subunit determines the ion selectivity of the GABAA receptor.
Authors Jensen Marianne L; Timmermann Daniel B; Johansen Tina H; Schousboe Arne; Varming Thomas; Ahring Philip K;
JournalJ Biol Chem
PubMed ID12177063
'The gamma-aminobutyric acid, type A (GABA(A)) receptor is a chloride-conducting receptor composed of alpha, beta, and gamma subunits assembled in a pentameric structure forming a central pore. Each subunit has a large extracellular agonist binding domain and four transmembrane domains (M1-M4), with the second transmembrane (M2) domain lining the pore. ... More
Characterization of the response element and DNA binding properties of the nuclear orphan receptor germ cell nuclear factor/retinoid receptor- related testis-associated receptor.
AuthorsYan ZH, Medvedev A, Hirose T, Gotoh H, Jetten AM
JournalJ Biol Chem
PubMed ID9099702
'Recently, we have reported the cloning of the germ cell-specific, nuclear orphan receptor germ cell nuclear factor (GCNF)/RTR. In this study, we characterize the RTR response elements by an electrophoretic mobility shift assay/polymerase chain reaction-based, DNA binding site selection strategy. RTR binds with the greatest affinity to response elements containing ... More
An mRNA surveillance mechanism that eliminates transcripts lacking termination codons.
Authors Frischmeyer Pamela A; van Hoof Ambro; O'Donnell Kathryn; Guerrerio Anthony L; Parker Roy; Dietz Harry C;
JournalScience
PubMed ID11910109
Translation is an important mechanism to monitor the quality of messenger RNAs (mRNAs), as exemplified by the translation-dependent recognition and degradation of transcripts harboring premature termination codons (PTCs) by the nonsense-mediated mRNA decay (NMD) pathway. We demonstrate in yeast that mRNAs lacking all termination codons are as labile as nonsense ... More
Subtraction hybridization identifies a transformation progression- associated gene PEG-3 with sequence homology to a growth arrest and DNA damage-inducible gene [published erratum appears in Proc Natl Acad Sci U S A 1997 Oct 28;94(22):12241]
AuthorsSu ZZ, Shi Y, Fisher PB
JournalProc Natl Acad Sci U S A
PubMed ID9256446
Cancer is a progressive multigenic disorder characterized by defined changes in the transformed phenotype that culminates in metastatic disease. Determining the molecular basis of progression should lead to new opportunities for improved diagnostic and therapeutic modalities. Through the use of subtraction hybridization, a gene associated with transformation progression in virus- ... More
Extension of life-span by introduction of telomerase into normal human cells [see comments]
AuthorsBodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu CP, Morin GB, Harley CB, Shay JW, Lichtsteiner S, Wright WE
JournalScience
PubMed ID9454332
Normal human cells undergo a finite number of cell divisions and ultimately enter a nondividing state called replicative senescence. It has been proposed that telomere shortening is the molecular clock that triggers senescence. To test this hypothesis, two telomerase-negative normal human cell types, retinal pigment epithelial cells and foreskin fibroblasts, ... More
Bilirubin, formed by activation of heme oxygenase-2, protects neurons against oxidative stress injury.
AuthorsDore S, Takahashi M, Ferris CD, Hester LD, Guastella D, Snyder SH
JournalProc Natl Acad Sci U S A
PubMed ID10051662
Heme oxygenase (HO) catalyzes the conversion of heme to carbon monoxide, iron, and biliverdin, which is immediately reduced to bilirubin (BR). Two HO active isozymes exist: HO1, an inducible heat shock protein, and HO2, which is constitutive and highly concentrated in neurons. We demonstrate a neuroprotective role for BR formed ... More
Requirement for either a host- or pectin-induced pectate lyase for infection of pisum sativum by nectriahematococca.
AuthorsRogers LM, Kim YK, Guo W, Gonzalez-Candelas L, Li D, Kolattukudy PE
JournalProc Natl Acad Sci U S A
PubMed ID10931947
Fungal pathogens usually have multiple genes that encode extracellular hydrolytic enzymes that may degrade the physical barriers in their hosts during the invasion process. Nectria hematococca, a plant pathogen, has two inducible pectate lyase (PL) genes (pel) encoding PL that can help degrade the carbohydrate barrier in the host. pelA ... More
A 40-bp RNA element that mediates stabilization of vascular endothelial growth factor mRNA by HuR.
Authors Goldberg-Cohen Ilana; Furneauxb Henry; Levy Andrew P;
JournalJ Biol Chem
PubMed ID11834731
VEGF is a critical mediator of hypoxia-induced angiogenesis in numerous physiological and pathophysiological conditions. The hypoxic induction of VEGF is due in large part to an increase in the stability of its mRNA. We recently demonstrated that the stabilization of VEGF mRNA by hypoxia is dependent upon the RNA-binding protein ... More
Regulation of angiotensin II-induced G protein signaling by phosducin-like protein.
Authors McLaughlin Joseph N; Thulin Craig D; Bray Steven M; Martin Mickey M; Elton Terry S; Willardson Barry M;
JournalJ Biol Chem
PubMed ID12107186
Phosducin-like protein (PhLP) is a broadly expressed member of the phosducin (Pd) family of G protein betagamma subunit (Gbetagamma)-binding proteins. Though PhLP has been shown to bind Gbetagamma in vitro, little is known about its physiological function. In the present study, the effect of PhLP on angiotensin II (Ang II) ... More