Forced expression of essential myosin light chain isoforms demonstrates their role in smooth muscle force production.
Authors Huang Q Q; Fisher S A; Brozovich F V;
JournalJ Biol Chem
PubMed ID10574990
'The molecular determinants of the contractile properties of smooth muscle are poorly understood, and have been suggested to be controlled by splice variant expression of the myosin heavy chain near the 25/50-kDa junction (Kelley, C. A., Takahashi, M., Yu, J. H., and Adelstein, R. S. (1993) J. Biol. Chem. 268, ... More
A region in domain II of the urokinase receptor required for urokinase binding.
Authors Bdeir K; Kuo A; Mazar A; Sachais B S; Xiao W; Gawlak S; Harris S; Higazi A A; Cines D B;
JournalJ Biol Chem
PubMed ID10864923
The urokinase receptor is composed of three homologous domains based on disulfide spacing. The contribution of each domain to the binding and activation of single chain urokinase (scuPA) remains poorly understood. In the present paper we examined the role of domain II (DII) in these processes. Repositioning DII to the ... More
Acylation of CD44 and its association with lipid rafts are required for receptor and hyaluronan endocytosis.
AuthorsThankamony SP, Knudson W,
JournalJ Biol Chem
PubMed ID16945930
CD44 is a cell surface receptor for the extracellular matrix macromolecule hyaluronan. In addition, CD44 mediates the endocytosis of hyaluronan leading to its subsequent degradation within lysosomes. Using model systems of COS-7 and Flp-293 cells, we demonstrate that the association of CD44 with lipid rafts is essential for the endocytosis ... More
Fusion of green fluorescent protein with the Zeocin-resistance marker allows visual screening and drug selection of transfected eukaryotic cells.
AuthorsBennett RP, Cox CA, Hoeffler JP
JournalAnal Biochem
PubMed ID9526661
Green fluorescent protein (GFP) and the Zeocin-resistance gene Sh ble (ZeoR) were fused together to generate a bifunctional protein for the identification and selection of transfected mammalian cells. Expression of this hybrid selectable marker, GFP-ZeoR, was visually detected and conferred Zeocin resistance in prokaryotes and eukaryotes. This selectable marker provides ... More