Green fluorescent protein-based system for analysis of E-selectin- mediated adhesion.
Authorsel-Battari A, Zerfaoui M, Panicot L, Mas E, Prevot C, Lombardo D
JournalBiotechniques
PubMed ID10090997
Numerous cell-based or cell-free systems for study of selectin adhesion use radiolabeled tracers. However, in addition to handling problems associated with the use of radioisotopes, these assays have difficulty relating a number of counts to a number of adherent cells. Here, we describe an assay that uses the natural fluorescence ... More
Fusion of green fluorescent protein with the Zeocin-resistance marker allows visual screening and drug selection of transfected eukaryotic cells.
AuthorsBennett RP, Cox CA, Hoeffler JP
JournalAnal Biochem
PubMed ID9526661
Green fluorescent protein (GFP) and the Zeocin-resistance gene Sh ble (ZeoR) were fused together to generate a bifunctional protein for the identification and selection of transfected mammalian cells. Expression of this hybrid selectable marker, GFP-ZeoR, was visually detected and conferred Zeocin resistance in prokaryotes and eukaryotes. This selectable marker provides ... More