Effects of the regulatory light chain phosphorylation of myosin II on mitosis and cytokinesis of mammalian cells.
Authors Komatsu S; Yano T; Shibata M; Tuft R A; Ikebe M;
JournalJ Biol Chem
PubMed ID10944522
'Myosin plays an important role in mitosis, especially during cytokinesis. Although it has been assumed that phosphorylation of regulatory light chain of myosin (RLC) controls motility of mammalian non-muscle cells, the functional significance of RLC phosphorylation remains uninvestigated. To address this problem, we have produced unphosphorylatable RLC (T18A/S19A RLC) and ... More
Identification of the erythropoietin receptor domain required for calcium channel activation.
AuthorsMiller BA, Barber DL, Bell LL, Beattie BK, Zhang MY, Neel BG, Yoakim M, Rothblum LI, Cheung JY
JournalJ Biol Chem
PubMed ID10400674
'Erythropoietin (Epo) activates a voltage-independent Ca2+ channel that is dependent on tyrosine phosphorylation. To identify the domain(s) of the Epo receptor (Epo-R) required for Epo-induced Ca2+ influx, Chinese hamster ovary (CHO) cells were transfected with wild-type or mutant Epo receptors subcloned into pTracer-cytomegalovirus vector. This vector contains an SV40 early ... More
5-Hydroxytryptamine 4(a) receptor is coupled to the Galpha subunit of heterotrimeric G13 protein.
'Serotonin (5-hydroxytryptamine (5-HT)) is an important neurotransmitter that regulates multiple events in the central nervous system. Many of the 5-HT functions are mediated via G protein-coupled receptors that are coupled to multiple heterotrimeric G proteins, including G(s), G(i), and G(q) subfamilies (Martin, G. R., Eglen, R. M., Hamblin, M. W., ... More
The regulation of anoikis: MEKK-1 activation requires cleavage by caspases.
AuthorsCardone MH, Salvesen GS, Widmann C, Johnson G, Frisch SM
JournalCell
PubMed ID9244305
Certain cell types undergo apoptosis when they lose integrin-mediated contacts with the extracellular matrix (anoikis). The Jun N-terminal kinase (JNK) pathway is activated in and promotes anoikis. This activation requires caspase activity. We presently report that a DEVD motif-specific caspase that cleaves MEKK-1 specifically is activated when cells lose matrix ... More
RYK, a catalytically inactive receptor tyrosine kinase, associates with EphB2 and EphB3 but does not interact with AF-6.
Authors Trivier Elisabeth; Ganesan Trivadi S;
JournalJ Biol Chem
PubMed ID11956217
RYK is an atypical orphan receptor tyrosine kinase that lacks detectable kinase activity. Nevertheless, using a chimeric receptor approach, we previously found that RYK can signal via the mitogen-activated protein kinase pathway. Recently, it has been shown that murine Ryk can bind to and be phosphorylated by the ephrin receptors ... More