pEXP2-DEST Vector Kit - FAQs

What is the difference between the pEXP1-DEST and pEXP2-DEST vectors that come in the Expressway kit?

These Gateway compatible vectors both have a T7 promoter and a T7 terminator. The pEXP1-DEST vector contains a ribosome binding site (RBS). If a RBS is present in your gene or as part of the entry vector, pEXP2-DEST can be used (since it does not have a RBS present). In addition, the pEXP1-DEST has an N-terminal 6xHis-Xpress fusion tag while the pEXP2-DEST vector has a C-terminal V5-6xHis fusion tag for convenient detection and purification of your recombinant proteins.