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View additional product information for Varioskan™ LUX multimode microplate reader, Absorbance and Fluorescence intensity - FAQs (VL0000D2)
21 product FAQs found
All of the DNA instruments offered by Thermo Fisher Scientific are of excellent quality. To find the right instrument to quantify DNA or RNA in your lab, explore the detailed comparison of UV-Vis spectrophotometers and fluorometers. Things like sensitivity, throughput and budget may be initial considerations when selecting a DNA quantification instrument. You may also consider whether you need target specificity, sample purity information, RNA quality information, or broad dynamic range.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.
UV and fluorescence technologies work differently to quantify DNA. UV quantification relies on the intrinsic absorptivity of DNA and RNA molecules, while fluorescence quantification uses dyes that specifically bind to your molecule of choice. With UV technology, quantification isn't as sensitive, but it has a broader dynamic range and also gives data about sample purity‒plus it is faster because there is no reagent prep. With fluorescence technology you get higher sensitivity and molecule-specific data, but it has lower dynamic range and reagent prep is required. The technology you need will depend on what features are important for your lab and experimentation.
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No, a PC is not included with purchase of the Varioskan LUX Multimode Microplate Reader.
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Yes, these functions are available. However, you will need to purchase SkanIt Drug Discovery Edition Software separately (Cat. No. 5187149).
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Yes, SkanIt Software Research Edition is included with purchase of the reader.
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Yes, the µDrop Plate can be used. Prewritten protocols are available in the SkanIt Software Cloud library.
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Yes, it uses 2 excitation monochromators and 2 emission monochromators.
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Typically, the lifespan is 10E9 flashes. This is equivalent to reading about 1 million 96-well microplates using 100ms measurement time per well.
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Pathlength correction is typically used when measuring DNA, RNA, or protein concentrations in the UV range.
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Up to 2 dispensers (injectors) can be ordered with the Varioskan LUX Multimode Microplate Reader.
They can be added to the reader after purchase, but they must be installed by a Thermo Fisher Scientific Field Service Engineer.
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In photometry mode, 6-well to 384-well plates can be used.
In all other read modes, 6-well to 1,536-well plates can be used.
Dispensing can be carried out with 6-well to 384-well plates.
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White plates are typically used for luminescence.
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Black plates generally provide the best results; they also have the lowest background fluorescence.
Transparent or white plates can be used, but the sensitivity is often lower.
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Yes, it can. To do so, you must purchase the robotic tray separately (Cat. No. N03079).
The SkanIt Automation Interface manual is available from your local support team.
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No, the gas control module must be included with the instrument at the time of purchase. Please contact your Thermo Fisher Scientific sales representative for further information.
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Yes, all these options can be added after purchase. However, they require upgrades performed by Thermo Fisher Scientific Field Service Engineers in your lab.
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The top of the plate, including any lid in use, cannot exceed the top rim of the plate tray.
The actual maximum height depends on which plate adapter is installed in the plate tray.
Adapters for plates with lids can be ordered separately.
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The Varioskan LUX Multimode Microplate Reader ships with adapters for use with 6-well to 48-well, 96-well and 384 well plates without lids.
Adapters for use of plates with lids as well as 1,536-well adapter for plate without lid and 96-well adaptor for PCR plate without lid can be ordered.
https://www.thermofisher.com/order/catalog/product/N02693?SID=srch-hj-N02693
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You are seeing this error because a waste strip was not detected in the plate tray.
The Varioskan LUX Multimode Microplate Reader ships with a strip plate. Remove one column of strip wells from the plate and insert into the slot on the left side of the plate tray to prevent the error message from appearing.
For details see Figure 2-15 on page 23 of the Varioskan LUX Multimode Microplate Reader technical manual.
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Absorbance and fluorescence intensity measurements do not use filters. For luminescence applications which require filters, users should purchase the filters separately and install them in the luminescence module.
Excitation filters for time-resolved fluorescence (TRF) and AlphaScreen are included when these options are ordered; however, emission filters must be purchased separately, as they can vary based on the user's application.
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In photometry mode, the wavelength range is 200 to 1000 nm (in 1 nm increments).
In fluorometry mode, the wavelength range using top reading is 200 to 1000 nm for excitation and 270 to 840 nm for emission (in 1 nm increments).
Ranges for other options can be found on our website at: https://www.thermofisher.com/us/en/home/life-science/lab-equipment/microplate-instruments/microplate-readers/varioskan-lux-multimode-microplate-reader.html
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.