Integrated expression of the a-amylase, dextranase and glutathione gene in an industrial brewer's yeast strain.
AuthorsWang JJ, Wang ZY, He XP, Zhang BR,
JournalWorld J Microbiol Biotechnol
PubMed ID22806798
Genetic engineering is widely used to meliorate biological characteristics of industrial brewing yeast. But how to solve multiple problems at one time has become the bottle neck in the genetic modifications of industrial yeast strains. In a newly constructed strain TYRL21, dextranase gene was expressed in addition of a-amylase to ... More
Pharmacological inhibition of ATM by KU55933 stimulates ATM transcription.
AuthorsKhalil HS, Tummala H, Hupp TR, Zhelev N,
JournalExp Biol Med (Maywood)
PubMed ID22728709
Ataxia-telangiectasia mutated (ATM) kinase is a component of a signalling mechanism that determines the process of decision-making in response to DNA damage and involves the participation of multiple proteins. ATM is activated by DNA double-strand breaks (DSBs) through the Mre11-Rad50-Nbs1 (MRN) DNA repair complex, and orchestrates signalling cascades that initiate ... More
A promoter polymorphism in the Per3 gene is associated with alcohol and stress response.
AuthorsWang X, Mozhui K, Li Z, Mulligan MK, Ingels JF, Zhou X, Hori RT, Chen H, Cook MN, Williams RW, Lu L,
JournalTransl Psychiatry
PubMed ID22832735
The period homolog genes Per1, Per2 and Per3 are important components of the circadian clock system. In addition to their role in maintaining circadian rhythm, these genes have been linked to mood disorders, stress response and vulnerability to addiction and alcoholism. In this study, we combined high-resolution sequence analysis and ... More
Indirubin-3'-oxime prevents hepatic I/R damage by inhibiting GSK-3beta and mitochondrial permeability transition.
'Indirubin-3''-oxime is an indirubin analogue that shows favorable inhibitory activity targeting glycogen synthase kinase 3beta (GSK-3beta). In this study, we evaluated if acute treatment with indirubin-3''-oxime (Ind) prevents hepatic ischemia/reperfusion (I/R) damage. Wistar rats were subjected to 150 min of 70% warm ischemia and 16 h of reperfusion. In the ... More
Physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening.
AuthorsMinas IS, Tanou G, Belghazi M, Job D, Manganaris GA, Molassiotis A, Vasilakakis M,
JournalJ Exp Bot
PubMed ID22268155
'Post-harvest ozone application has recently been shown to inhibit the onset of senescence symptoms on fleshy fruit and vegetables; however, the exact mechanism of action is yet unknown. To characterize the impact of ozone on the post-harvest performance of kiwifruit (Actinidia deliciosa cv. ''Hayward''), fruits were cold stored (0 °C, ... More
DNA damage in embryonic stem cells caused by nanodiamonds.
AuthorsXing Y, Xiong W, Zhu L, Osawa E, Hussin S, Dai L,
JournalACS Nano
PubMed ID21370893
'Because of their unique photoluminescence and magnetic properties, nanodiamonds (NDs) are promising for biomedical imaging and therapeutical applications. However, these biomedical applications will hardly be realized unless the potential hazards of NDs to humans and other biological systems are ascertained. Previous studies performed in our group and others have demonstrated ... More
High prevalence of laminopathies among patients with metabolic syndrome.
AuthorsDutour A, Roll P, Gaborit B, Courrier S, Alessi MC, Tregouet DA, Angelis F, Robaglia-Schlupp A, Lesavre N, Cau P, Lévy N, Badens C, Morange PE,
JournalHum Mol Genet
PubMed ID21724554
'Constitutional laminopathies, such as the Dunnigan familial partial lipodystrophy, are severe diseases caused by mutations in A-type lamins and share several features with metabolic syndrome (MS). In this study, we hypothesized that MS may be, in some cases, a mild form of laminopathies and use the abnormal cell nucleus phenotype ... More
Phosphorylated fetuin-A-containing calciprotein particles are associated with aortic stiffness and a procalcific milieu in patients with pre-dialysis CKD.
AuthorsSmith ER, Ford ML, Tomlinson LA, Rajkumar C, McMahon LP, Holt SG,
JournalNephrol Dial Transplant
PubMed ID22105144
'Vascular stiffening occurs in normal ageing and is accelerated in chronic kidney disease (CKD). Vascular calcification contributes to this stiffening and to the high incidence of vascular morbidity and mortality in this population. A network of inhibitors work in concert to reduce mineralization risk in extra-osseous tissue. Fetuin-A is an ... More
Microarray analysis of the primate luteal transcriptome during chorionic gonadotrophin administration simulating early pregnancy.
'To explore chorionic gonadotrophin (CG)-regulated gene expression in the primate corpus luteum (CL), adult female rhesus macaques were treated in a model of simulated early pregnancy (SEP). Total RNA was isolated from individual CL after specific intervals of exposure (1, 3, 6 and 9 days) to recombinant hCG in vivo ... More
PTRF/cavin-1 and MIF proteins are identified as non-small cell lung cancer biomarkers by label-free proteomics.
AuthorsGámez-Pozo A, Sánchez-Navarro I, Calvo E, Agulló-Ortuño MT, López-Vacas R, Díaz E, Camafeita E, Nistal M, Madero R, Espinosa E, López JA, Fresno Vara JÁ,
JournalPLoS One
PubMed ID22461895
With the completion of the human genome sequence, biomedical sciences have entered in the
Subspecies IIIa and IIIb Salmonellae are Defective for Colonization of Murine Models of Salmonellosis as Compared to Ssp. I Serotype Typhimurium.
AuthorsKatribe E, Bogomolnaya LM, Wingert H, Andrews-Polymenis H,
JournalJ Bacteriol
PubMed ID19218378
Non-subspecies I Salmonellae are commensals of cold-blooded vertebrates and cause sporadic disease in mammals. The reasons why non-ssp. I Salmonellae do not circulate in populations of warm-blooded vertebrates, but instead only cause occasional disease in this niche, are unknown. We examined the ability of ssp. IIIa Arizonae and ssp. IIIb ... More
Recombinant retrovirus-derived virus-like particle-based vaccines induce hepatitis C virus-specific cellular and neutralizing immune responses in mice.
AuthorsHuret C, Desjardins D, Miyalou M, Levacher B, Amadoudji Zin M, Bonduelle O, Combadière B, Dalba C, Klatzmann D, Bellier B,
JournalVaccine
PubMed ID22634300
While the immunological correlates of hepatitis C virus (HCV)-specific immunity are not well understood, it is now admitted that an effective vaccine against HCV will need to induce both cellular and humoral immune responses and address viral heterogeneity to prevent immune escape. We developed a vaccine platform specifically aimed at ... More
Pattern of expression of vascular endothelial growth factor and its receptors in the ovine choroid plexus during long and short photoperiods.
AuthorsSzczepkowska A, Wasowska B, Gilun PD, Lagaraine C, Robert V, Dufourny L, Thiéry JC, Skipor J,
JournalCell Tissue Res
PubMed ID22622803
Vascular endothelial growth factor (VEGF-A) plays an important role in maintaining cerebrospinal fluid (CSF) homeostasis and the function of the choroid plexuses (CPs). The objective of the study was to determine the expression of vascular endothelial growth factor (VEGF-A), tyrosine kinase receptors Flt-1 and KDR and KDR co-receptor neuropilin 1 ... More
DNA damage response in imatinib resistant chronic myeloid leukemia K562 cells.
AuthorsDinis J, Silva V, Gromicho M, Martins C, Laires A, Tavares P, Rendeiro P, Torres F, Rueff J, Rodrigues A,
JournalLeuk Lymphoma
PubMed ID22475343
Resistance to imatinib in patients with chronic myeloid leukemia can lead to advanced disease and blast crisis. Conventional chemotherapy with DNA damaging agents is then used, alone or in combination with other tyrosine kinase inhibitors (TKIs). Our aim was to assess whether imatinib resistant K562 cells were also resistant to ... More
The transcriptome and proteome are altered in marine polychaetes (Annelida) exposed to elevated metal levels.
Polychaetes are often used in toxicological studies to understand mechanisms of resistance and for biomarker detection, however, we know of only a few genetic pathways involved in resistance. We found the marine polychaete Ophelina sp.1 (Opheliidae) in sediment containing high copper levels and investigated this phenomenon by measuring metal accumulation ... More
Assessment of inactivated human rabies vaccines: biochemical characterization and genetic identification of virus strains.
AuthorsFinke S, Karger A, Freuling C, Müller T,
JournalVaccine
PubMed ID22469862
The World Health Organization (WHO) recommends the periodic evaluation of the purity of the cell lines used in the production of rabies vaccines, as well as the antigenic identity of the virus strains. Here, we analyzed seventeen marketed inactivated human rabies virus vaccines for viral and non-viral proteins by SDS-PAGE ... More
Overexpression and potential targeting of the oncofoetal antigen 5T4 in malignant pleural mesothelioma.
AuthorsAl-Taei S, Salimu J, Lester JF, Linnane S, Goonewardena M, Harrop R, Mason MD, Tabi Z,
JournalLung Cancer
PubMed ID22498111
Malignant pleural mesothelioma (MPM) is resistant to conventional treatments. Novel, targeted treatments are hampered by the relative lack of MPM-associated tumour antigens. The aim of this study was to evaluate the level of expression and the relevance of 5T4 as a tumour-associated antigen in MPM. 5T4 expression was assessed by ... More
Altered human CYP3A4 activity caused by Antley-Bixler syndrome-related variants of NADPH-cytochrome P450 oxidoreductase measured in a robust in vitro system.
AuthorsMoutinho D, Marohnic CC, Panda SP, Rueff J, Masters BS, Kranendonk M,
JournalDrug Metab Dispos
PubMed ID22252407
NADPH-cytochrome P450 oxidoreductase (CYPOR) variants have been described in patients with perturbed steroidogenesis and sexual differentiation, related to Antley-Bixler syndrome (ABS). It is important to determine the effect of these variants on CYP3A4, the major drug-metabolizing cytochrome P450 (P450) in humans. In this study, 12 CYPOR_ABS variants were separately coexpressed ... More
Selection of putative colorectal cancer markers by applying PCA on the soluble proteome of tumors: NDK A as a promising candidate.
AuthorsAlvarez-Chaver P, Rodríguez-Piñeiro AM, Rodríguez-Berrocal FJ, García-Lorenzo A, Páez de la Cadena M, Martínez-Zorzano VS,
JournalJ Proteomics
PubMed ID21385632
Aiming to find new tumor markers for colorectal cancer (CRC), we applied proteomic methodologies to compare the soluble sub-proteome of healthy and tumoral colorectal mucosa. Out of 91 differentially expressed proteins, 23 were selected by principal component analysis (PCA) as the major contributors to the overall difference detected. After MS/MS ... More
The role of cell surface markers and enamel matrix derivatives on human periodontal ligament mesenchymal progenitor responses in vitro.
Periodontitis is a chronic-, infectious-disease of the human periodontium that is characterized by the loss of supporting tissues surrounding the tooth such as the periodontal ligament (PDL), cementum and alveolar bone. Regeneration of the periodontium is dependent on the participation of mesenchymal stem/stromal cells (MSC) resident in the PDL. Enamel ... More
Atorvastatin activates heme oxygenase-1 at the stress response elements.
AuthorsKwok SC, Samuel SP, Handal J,
JournalJ Cell Mol Med
PubMed ID21447045
Statins are known to inhibit growth of a number of cancer cells, but their mechanism of action is not well established. In this study, human prostate adenocarcinoma PC-3 and breast adenocarcinoma MCF-7 cell lines were used as models to investigate the mechanism of action of atorvastatin, one of the statins. ... More
Toll-like receptor signaling pathway in chronic lymphocytic leukemia: distinct gene expression profiles of potential pathogenic significance in specific subsets of patients.
AuthorsArvaniti E, Ntoufa S, Papakonstantinou N, Touloumenidou T, Laoutaris N, Anagnostopoulos A, Lamnissou K, Caligaris-Cappio F, Stamatopoulos K, Ghia P, Muzio M, Belessi C,
JournalHaematologica
PubMed ID21750087
Signaling through the B-cell receptor appears to be a major contributor to the pathogenesis of chronic lymphocytic leukemia. Toll-like receptors bridge the innate and adaptive immune responses by acting as co-stimulatory signals for B cells. The available data on the expression of Toll-like receptors in chronic lymphocytic leukemia are limited ... More
Harnessing the glucosyltransferase activities of Clostridium difficile for functional studies of toxins A and B.
AuthorsDarkoh C, Kaplan HB, Dupont HL,
JournalJ Clin Microbiol
PubMed ID21653766
The incidence of Clostridium difficile infection (CDI) has been increasing within the last decade. Pathogenic strains of C. difficile produce toxin A and/or toxin B, which are important virulence factors in the pathogenesis of this bacterium. Current methods for diagnosing CDI are mostly qualitative tests that detect either the bacterium ... More
Foxp3 expression in liver correlates with the degree but not the cause of inflammation.
AuthorsSpeletas M, Argentou N, Germanidis G, Vasiliadis T, Mantzoukis K, Patsiaoura K, Nikolaidis P, Karanikas V, Ritis K, Germenis AE,
JournalMediators Inflamm
PubMed ID21772667
Patients with chronic viral hepatitis display increased expression of Foxp3 in liver, suggesting that Tregs expansion contributes to persistent infection. The purpose of this study was to elucidate whether the expression of Foxp3 relates not to the viral infection but to the resulting liver inflammation. Liver biopsies obtained from 69 ... More
Biochemical and morphological consequences of human a-synuclein expression in a mouse a-synuclein null background.
AuthorsPrasad K, Tarasewicz E, Strickland PA, O'Neill M, Mitchell SN, Merchant K, Tep S, Hilton K, Datwani A, Buttini M, Mueller-Steiner S, Richfield EK,
JournalEur J Neurosci
PubMed ID21272100
A consensus about the functions of human wild-type or mutated a-synuclein (aSYN) is lacking. Both forms of aSYN are implicated in Parkinson's disease, whereas the wild-type form is implicated in substance abuse. Interactions with other cellular proteins and organelles may meditate its functions. We developed a series of congenic mouse ... More
Tonotopic changes in GABA receptor expression in guinea pig inferior colliculus after partial unilateral hearing loss.
AuthorsDong S, Rodger J, Mulders WH, Robertson D,
JournalBrain Res
PubMed ID20438718
Immunohistochemistry was used to investigate the topographic distribution of the alpha1 subunit of the GABA receptor (GABRA1) in guinea pig inferior colliculus after treatments that caused a unilateral loss of peripheral neural sensitivity in the high-frequency regions of the cochlea. Both forms of treatment (direct mechanical lesion of the cochlea ... More
The effects of luteinizing hormone ablation/replacement versus steroid ablation/replacement on gene expression in the primate corpus luteum.
AuthorsBishop CV, Hennebold JD, Stouffer RL,
JournalMol Hum Reprod
PubMed ID19168862
This study was designed to provide a genome-wide analysis of the effects of luteinizing hormone (LH) versus steroid ablation/replacement on gene expression in the developed corpus luteum (CL) in primates during the menstrual cycle. On Days 9-11 of the luteal phase, female rhesus monkeys were left untreated (control) or received ... More