Essential role of protein kinase C delta in platelet signaling, alpha IIb beta 3 activation, and thromboxane A2 release.
AuthorsYacoub D, Théorêt JF, Villeneuve L, Abou-Saleh H, Mourad W, Allen BG, Merhi Y
JournalJ Biol Chem
PubMed ID16895913
'The protein kinase C (PKC) family is an essential signaling mediator in platelet activation and aggregation. However, the relative importance of the major platelet PKC isoforms and their downstream effectors in platelet signaling and function remain unclear. Using isolated human platelets, we report that PKCdelta, but not PKCalpha or PKCbeta, ... More
Polarized insertion of new membrane from a cytoplasmic reservoir during cleavage of the Drosophila embryo.
AuthorsLecuit T, Wieschaus E
JournalJ Cell Biol
PubMed ID10953008
'Cellularization of the Drosophila embryo is a specialized form of cytokinesis that results in the formation of a polarized epithelium. The mechanisms of membrane growth during cytokinesis are largely unknown. It is also unclear whether membrane growth and polarization represent distinct processes that occur simultaneously or whether growth of the ... More
Dysmorphic photoreceptors in a P23H mutant rhodopsin model of retinitis pigmentosa are metabolically active and capable of regenerating to reverse retinal degeneration.
AuthorsLee DC, Vazquez-Chona FR, Ferrell WD, Tam BM, Jones BW, Marc RE, Moritz OL,
JournalJ Neurosci
PubMed ID22323724
'This study evaluated the capacity of Xenopus laevis retina to regenerate photoreceptor cells after cyclic light-mediated acute rod photoreceptor degeneration in a transgenic P23H mutant rhodopsin model of retinits pigmentosa. After discontinuation of cyclic light exposure, we monitored histologic progression of retinal regeneration over a 3 week recovery period. To ... More
Lectin mapping reveals stage-specific display of surface carbohydrates in in vitro and haemolymph-derived cells of the entomopathogenic fungus Beauveria bassiana.
AuthorsWanchoo A, Lewis MW, Keyhani NO,
JournalMicrobiology
PubMed ID19608611
'The entomopathogenic fungus Beauveria bassiana and its insect host target represent a model system with which to examine host-pathogen interactions. Carbohydrate epitopes on the surfaces of fungal cells play diverse roles in processes that include adhesion, non-self recognition and immune invasion with respect to invertebrate hosts. B. bassiana produces a ... More
Histological parameters for the quantitative assessment of muscular dystrophy in the mdx-mouse.
AuthorsBriguet A, Courdier-Fruh I, Foster M, Meier T, Magyar JP,
JournalNeuromuscul Disord
PubMed ID15351425
'Duchenne muscular dystrophy is a severe X-linked hereditary disease caused by the absence of functional dystrophin. The dystrophin-deficient mdx-mouse strain is a widely used animal model for dystrophin-deficiency. Several therapeutic approaches for muscular dystrophy have been proposed by different laboratories. In order to compare the efficacy of these therapies in ... More
A Medicago truncatula phosphate transporter indispensable for the arbuscular mycorrhizal symbiosis.
AuthorsJavot H, Penmetsa RV, Terzaghi N, Cook DR, Harrison MJ
JournalProc Natl Acad Sci U S A
PubMed ID17242358
'The arbuscular mycorrhizal (AM) symbiosis is a mutualistic endosymbiosis formed by plant roots and AM fungi. Most vascular flowering plants have the ability to form these associations, which have a significant impact on plant health and consequently on ecosystem function. Nutrient exchange is a central feature of the AM symbiosis, ... More
Liposome-mediated RNA transfection should be used with caution.
Liposome-mediated RNA transfection appears to present a number of advantages for studying the metabolism of reporter mRNAs in mammalian cells. This method is also widely used to transfect siRNAs. Here we describe results indicating that reporter mRNAs introduced into HeLa cells by liposomes do not present the expected behaviors. Namely, ... More
3-D imaging and illustration of mouse intestinal neurovascular complex.
AuthorsFu YY, Peng SJ, Lin HY, Pasricha PJ, Tang SC,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID23086917
Because of the dispersed nature of nerves and blood vessels, standard histology cannot provide a global and associated observation of the enteric nervous system (ENS) and vascular network. We prepared transparent mouse intestine and combined vessel painting and three-dimensional (3-D) neurohistology for joint visualization of the ENS and vasculature. Cardiac ... More
Regulation of ASIC channels by a stomatin/STOML3 complex located in a mobile vesicle pool in sensory neurons.
AuthorsLapatsina L, Jira JA, Smith ES, Poole K, Kozlenkov A, Bilbao D, Lewin GR, Heppenstall PA,
JournalOpen Biol
PubMed ID22773952
A complex of stomatin-family proteins and acid-sensing (proton-gated) ion channel (ASIC) family members participate in sensory transduction in invertebrates and vertebrates. Here, we have examined the role of the stomatin-family protein stomatin-like protein-3 (STOML3) in this process. We demonstrate that STOML3 interacts with stomatin and ASIC subunits and that this ... More
Measurement of wheat germ agglutinin binding with a fluorescence microscope.
AuthorsModel MA, Reese JL, Fraizer GC,
JournalCytometry A
PubMed ID19722258
Signal intensity in fluorescence microscopy is often measured relative to arbitrary standards. We propose a calibration method based on a solution of the same fluorophore, whose binding to cells needs to be quantified. The method utilizes the low sensitivity of intensity to the object distance in wide-field imaging of uniform ... More
Cloning, expression, and functional characterization of a Ca(2+)-dependent endoplasmic reticulum nucleoside diphosphatase.
AuthorsFailer BU, Braun N, Zimmermann H,
JournalJ Biol Chem
PubMed ID12167635
We have isolated and characterized the cDNA encoding a Ca(2+)-dependent nucleoside diphosphatase (EC ) related to two secreted ATP- and ADP-hydrolyzing apyrases of the bloodsucking insects, Cimex lectularius and Phlebotomus papatasi. The rat brain-derived cDNA has an open reading frame of 1209 bp encoding a protein of 403 amino acids ... More
Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells.
AuthorsShalek AK, Robinson JT, Karp ES, Lee JS, Ahn DR, Yoon MH, Sutton A, Jorgolli M, Gertner RS, Gujral TS, Macbeath G, Yang EG, Park H,
JournalProc Natl Acad Sci U S A
PubMed ID20080678
A generalized platform for introducing a diverse range of biomolecules into living cells in high-throughput could transform how complex cellular processes are probed and analyzed. Here, we demonstrate spatially localized, efficient, and universal delivery of biomolecules into immortalized and primary mammalian cells using surface-modified vertical silicon nanowires. The method relies ... More
Multiparameter assessments to determine the effects of sugars and antimicrobials on a polymicrobial oral biofilm.
AuthorsYang Y, Sreenivasan PK, Subramanyam R, Cummins D,
JournalAppl Environ Microbiol
PubMed ID17021225
Clinical studies indicate relationships between dental plaque, a naturally formed biofilm, and oral diseases. The crucial role of nonmicrobial biofilm constituents in maintaining biofilm structure and biofilm-specific attributes, such as resistance to shear and viscoelasticity, is increasingly recognized. Concurrent analyses of the diverse nonmicrobial biofilm components for multiparameter assessments formed ... More
Selective chemical treatment of cellular microdomains using multiple laminar streams.
AuthorsTakayama S, Ostuni E, LeDuc P, Naruse K, Ingber DE, Whitesides GM
JournalChem Biol
PubMed ID12618184
There are many experiments in which it would be useful to treat a part of the surface or interior of a cell with a biochemical reagent. It is difficult, however, to achieve subcellular specificity, because small molecules diffuse distances equal to the extent of the cell in seconds. This paper ... More
Copper and zinc cause delivery of the prion protein from the plasma membrane to a subset of early endosomes and the Golgi.
AuthorsBrown LR, Harris DA
JournalJ Neurochem
PubMed ID14511113
The cellular isoform of prion protein (PrPC) is a plasma membrane glycoprotein whose conformational conversion into PrPSc is the central molecular event in the propagation of infectious prions. However, the physiological function of PrPC has remained uncertain. The finding that PrPC binds copper ions with low micromolar affinity, coupled with ... More
Functional and selective RNA interference in developing axons and growth cones.
AuthorsHengst U, Cox LJ, Macosko EZ, Jaffrey SR,
JournalJ Neurosci
PubMed ID16723529
Developing axons and growth cones contain “local” mRNAs that are translated in response to various extracellular signaling molecules and have roles in several processes during axonal development, including axonal pathfinding, orientation of axons in chemotactic gradients, and in the regulation of neurotransmitter release. The molecular mechanisms that regulate mRNA translation ... More
Direct reconstitution of plasma membrane lipids and proteins in nanotube-vesicle networks.
AuthorsBauer B, Davidson M, Orwar O
JournalLangmuir
PubMed ID17042549
We demonstrate here that nanotube-vesicle networks can be constructed directly from plasma membranes of cultured cells. We used a combination of dithiothreitol (DTT) and formaldehyde to produce micron-sized plasma membrane vesicles that were subsequently shaped into networks using micromanipulation methods previously used on purely synthetic systems. Only a single cell ... More
The root endophytic fungus Piriformospora indica requires host cell death for proliferation during mutualistic symbiosis with barley.
AuthorsDeshmukh S, Hückelhoven R, Schäfer P, Imani J, Sharma M, Weiss M, Waller F, Kogel KH
JournalProc Natl Acad Sci U S A
PubMed ID17116870
Fungi of the recently defined order Sebacinales (Basidiomycota) are involved in a wide spectrum of mutualistic symbioses (including mycorrhizae) with various plants, thereby exhibiting a unique potential for biocontrol strategies. The axenically cultivable root endophyte Piriformospora indica is a model organism of this fungal order. It is able to increase ... More
Deep Learning Reveals Cancer Metastasis and Therapeutic Antibody Targeting in the Entire Body.
Authors
JournalCell
PubMed ID31835038
Peptide vaccine for semaphorin3E ameliorates systemic glucose intolerance in mice with dietary obesity.
AuthorsYoshida Y, Shimizu I, Hayashi Y, Ikegami R, Suda M, Katsuumi G, Wakasugi T, Nakao M, Nakagami H, Morishita R, Minamino T
JournalSci Rep
PubMed ID30846754
'We previously demonstrated that cellular aging signals upregulated a secreted class 3 semaphorin E (Sema3E) and its receptor plexinD1 in the adipose tissue of a murine model of dietary obesity and that Sema3E was a chemoattractant, mediating its biological effects by inducing infiltration of plexinD1-positive inflammatory macrophages into the visceral ... More
In Situ Modification of Tissue Stem and Progenitor Cell Genomes.
AuthorsGoldstein JM, Tabebordbar M, Zhu K, Wang LD, Messemer KA, Peacker B, Ashrafi Kakhki S, Gonzalez-Celeiro M, Shwartz Y, Cheng JKW, Xiao R, Barungi T, Albright C, Hsu YC, Vandenberghe LH, Wagers AJ
JournalCell Rep
PubMed ID31018138
'In vivo delivery of genome-modifying enzymes holds significant promise for therapeutic applications and functional genetic screening. Delivery to endogenous tissue stem cells, which provide an enduring source of cell replacement during homeostasis and regeneration, is of particular interest. Here, we use a sensitive Cre/lox fluorescent reporter system to test the efficiency ... More
Deletion of caveolin scaffolding domain alters cancer cell migration.
AuthorsOkada S, Raja SA, Okerblom J, Boddu A, Horikawa Y, Ray S, Okada H, Kawamura I, Murofushi Y, Murray F, Patel HH
JournalCell Cycle
PubMed ID31116089
'Caveolin-1 (Cav-1) is an integral membrane protein that plays an important role in proliferative and terminally differentiated cells. As a structural component of Caveolae, Cav-1 interacts with signaling molecules via a caveolin scaffolding domain (CSD) regulating cell signaling. Recent reports have shown that Cav-1 is a negative regulator in tumor ... More
Phenome-based approach identifies RIC1-linked Mendelian syndrome through zebrafish models, biobank associations and clinical studies.
Authors
JournalNat Med
PubMed ID31932796
Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption.
Authors
JournalCell
PubMed ID33636130
H2A.Z is dispensable for both basal and activated transcription in post-mitotic mouse muscles.
Authors
JournalNucleic Acids Res
PubMed ID32266374
Frequency of mononuclear diploid cardiomyocytes underlies natural variation in heart regeneration.
Authors
JournalNat Genet
PubMed ID28783163
A subset of chondrogenic cells provides early mesenchymal progenitors in growing bones.
Authors
JournalNat Cell Biol
PubMed ID25419849
Advanced maturation of human cardiac tissue grown from pluripotent stem cells.
Authors
JournalNature
PubMed ID29618819
FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis.
Authors
JournalCell
PubMed ID27839864
MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.
Authors
JournalCell
PubMed ID27768893
Transmembrane Pickets Connect Cyto- and Pericellular Skeletons Forming Barriers to Receptor Engagement.
Authors
JournalCell
PubMed ID29328918
Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and improves regenerative capacity of aged skeletal muscle.
Aging is accompanied by progressive declines in skeletal muscle mass and strength and impaired regenerative capacity, predisposing older adults to debilitating age-related muscle deteriorations and severe morbidity. Muscle stem cells (muSCs) that proliferate, differentiate to fusion-competent myoblasts, and facilitate muscle regeneration are increasingly dysfunctional upon aging, impairing muscle recovery after ... More
Draxin alters laminin organization during basement membrane remodeling to control cranial neural crest EMT.
AuthorsHutchins EJ, Bronner ME
JournalDev Biol
PubMed ID30579765
Premigratory neural crest cells arise within the dorsal neural tube and subsequently undergo an epithelial-to-mesenchymal transition (EMT) to leave the neuroepithelium and initiate migration. Draxin is a Wnt modulator that has been shown to control the timing of cranial neural crest EMT. Here we show that this process is accompanied ... More
Engineering of human cardiac muscle electromechanically matured to an adult-like phenotype.
AuthorsRonaldson-Bouchard K, Yeager K, Teles D, Chen T, Ma S, Song L, Morikawa K, Wobma HM, Vasciaveo A, Ruiz EC, Yazawa M, Vunjak-Novakovic G
JournalNat Protoc
PubMed ID31492957
The application of tissue-engineering approaches to human induced pluripotent stem (hiPS) cells enables the development of physiologically relevant human tissue models for in vitro studies of development, regeneration, and disease. However, the immature phenotype of hiPS-derived cardiomyocytes (hiPS-CMs) limits their utility. We have developed a protocol to generate engineered cardiac ... More
Beta3-Tubulin is Critical for Microtubule Dynamics, Cell Cycle Regulation, and Spontaneous Release of Microvesicles in Human Malignant Melanoma Cells (A375).
AuthorsAltonsy MO, Ganguly A, Amrein M, Surmanowicz P, Li SS, Lauzon GJ, Mydlarski PR
JournalInt J Mol Sci
PubMed ID32121295
Microtubules (MTs), microfilaments, and intermediate filaments, the main constituents of the cytoskeleton, undergo continuous structural changes (metamorphosis), which are central to cellular growth, division, and release of microvesicles (MVs). Altered MTs dynamics, uncontrolled proliferation, and increased production of MVs are hallmarks of carcinogenesis. Class III beta-tubulin (ß3-tubulin), one of seven ... More