'Prominin-1 (CD133) is commonly used to isolate stem and progenitor cells from the developing and adult nervous system and to identify cancer stem cells in brain tumors. However, despite extensive characterization of Prominin-1(+) precursor cells from the adult subventricular zone, no information about the expression of Prominin-1 by precursor cells ... More
Differential regulation by cyclic nucleotides of the CNGA4 and CNGB1b subunits in olfactory cyclic nucleotide-gated channels.
Olfactory cyclic nucleotide-gated (CNG) ion channels are essential contributors to signal transduction of olfactory sensory neurons. The activity of the channels is controlled by the cyclic nucleotides guanosine 3',5'-monophosphate (cGMP) and adenosine 3',5'-monophosphate (cAMP). The olfactory CNG channels are composed of two CNGA2 subunits, one CNGA4 and one CNGB1b subunit, ... More
Membrane lipid composition and its physicochemical properties define cell vulnerability to aberrant protein oligomers.
AuthorsEvangelisti E, Cecchi C, Cascella R, Sgromo C, Becatti M, Dobson CM, Chiti F, Stefani M,
JournalJ Cell Sci
PubMed ID22344258
Increasing evidence suggests that the interaction of misfolded protein oligomers with cell membranes is a primary event resulting in the cytotoxicity associated with many protein-misfolding diseases, including neurodegenerative disorders. We describe here the results of a study on the relative contributions to toxicity of the physicochemical properties of protein oligomers ... More
Cytosolic entry of bisphosphonate drugs requires acidification of vesicles after fluid-phase endocytosis.
Bisphosphonates such as alendronate and zoledronate are blockbuster drugs used to inhibit osteoclast-mediated bone resorption. Although the molecular mechanisms by which bisphosphonates affect osteoclasts are now evident, the exact route by which they are internalized by cells is not known. To clarify this, we synthesized a novel, fluorescently labeled analog ... More
Cationic albumin-conjugated pegylated nanoparticles allow gene delivery into brain tumors via intravenous administration.
AuthorsLu W, Sun Q, Wan J, She Z, Jiang XG
JournalCancer Res
PubMed ID17178885
Patients with malignant gliomas have a poor prognosis because these tumors do not respond well to conventional treatments. Studies of glioma xenografts suggest that they may be amenable to gene therapy with cytotoxic genes, such as the proapoptotic Apo2 ligand/tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL). Gene therapy of gliomas ideally ... More
S-nitrosoprotein formation and localization in endothelial cells.
AuthorsYang Y, Loscalzo J
JournalProc Natl Acad Sci U S A
PubMed ID15618409
Protein S-nitrosation represents a recently described form of post-translational modification that is rapid and reversible. However, the analysis of protein S-nitrosation in situ has been difficult because of the absence of specific probes and the instability of cellular protein S-nitrosothiols. We developed a rapid and specific method for detecting endothelial ... More
Structural Basis of BRCC36 Function in DNA Repair and Immune Regulation.
Authors
JournalMol Cell
PubMed ID31253574
Localized force application reveals mechanically sensitive domains of Piezo1.
Authors
JournalNat Commun
PubMed ID27694883
Reactogenicity of live-attenuated Vibrio cholerae vaccines is dependent on flagellins.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID20160087
In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration.
Authors
JournalElife
PubMed ID33764296
Characterizing Nanoparticles in Biological Matrices: Tipping Points in Agglomeration State and Cellular Delivery In Vitro.
Authors
JournalACS Nano
PubMed ID29072897
The release of toxic oligomers from α-synuclein fibrils induces dysfunction in neuronal cells.
Authors
JournalNat Commun
PubMed ID33753734
Human mesenchymal stromal cells transplanted into mice stimulate renal tubular cells and enhance mitochondrial function.
Authors
JournalNat Commun
PubMed ID29042548
Glycan-Independent Gamete Recognition Triggers Egg Zinc Sparks and ZP2 Cleavage to Prevent Polyspermy.
Authors
JournalDev Cell
PubMed ID30122633
Obesity-associated hyperleptinemia alters the gliovascular interface of the hypothalamus to promote hypertension.
Authors
JournalCell Metab
PubMed ID33951475
MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.