WesternBreeze™ 洗涤液 (16X)

WesternBreeze® 洗涤液 (16X) 是一种含去污剂的浓缩缓冲盐水的优化溶液，将在硝酸纤维素 (NC) 和聚偏二氟乙烯 (PVDF) 膜上的背景和非特异性结合最小化。有足够用于 20了解更多信息

货号	数量
WB7003	2 x 100 mL

货号 WB7003

价格（CNY)

1,968.00

2 x 100 mL

添加至购物车

2 x 100 mL

价格（CNY)

1,968.00

2 x 100 mL

添加至购物车

WesternBreeze® 洗涤液 (16X) 是一种含去污剂的浓缩缓冲盐水的优化溶液，将在硝酸纤维素 (NC) 和聚偏二氟乙烯 (PVDF) 膜上的背景和非特异性结合最小化。有足够用于 20 次微型印迹的试剂。

仅供科研使用。不可用于诊断程序。

缓冲液印迹缓冲液

数量2 x 100 mL

膜兼容性硝酸纤维素、PVDF

产品线WesternBreeze

产品类型清洗液

Unit Size2 x 100 mL

内容与储存

2 x 100 mL 抗体洗涤液 (16X)

在 +4°C 下储存。

常见问题解答 (FAQ)

WesternBreeze化学发光检测试剂盒中的封闭液/稀释液和抗体洗涤液是否可以作为独立产品购买？

可以，您可使用以下货号单独购买这些产品：

•货号WB7001（封闭液/稀释液A）
•货号WB7002（封闭液/稀释液B）
•货号WB7050（封闭液/稀释液A & B组合装）
•货号WB7003（抗体洗涤液）

WesternBreeze显色法检测试剂盒中的封闭液/稀释液和抗体洗涤液是否可以作为独立产品购买？

可以，您可使用以下货号单独购买这些产品：

•货号WB7003（抗体洗涤液）
•货号WB7001（封闭液/稀释液A）
•货号WB7002（封闭液/稀释液B）
•货号WB7050（封闭液/稀释液A & B组合装）

Why is the actual band size on a western blot different from the predicted size of the protein?

Western blotting is based on the separation of proteins by their size on a gel. However, migration of proteins through the gel matrix is also affected by other factors, which may cause the observed band size to be different from the predicted size.

Common causes are:
-Post-translational modification; for example phosphorylation and glycosylation increase the size of the protein
-Post-translation cleavage; many proteins are synthesized as precursor proteins, and then cleaved to give the active form
-Multimers, for example dimerization of a protein. This is usually prevented under reducing conditions, although strong interactions can result in the appearance of higher bands
-Splice variants; alternative splicing may result in different sized proteins being produced from the same gene
-Relative charge; the composition of amino acids (charged vs. non-charged)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Can I purchase the Blocker/Diluent and Antibody Wash solutions from the WesternBreeze Chemiluminescent Detection kits as standalone products?

Yes, you may purchase them as standalone products using the Cat. Nos. listed below:

- Cat. No. WB7001 (Blocker/Diluent A)
- Cat. No. WB7002 (Blocker/Diluent B)
- Cat. No. WB7050 (Combo pack containing Blocker/Diluents A & B)
- Cat. No. WB7003 (Antibody Wash)

Note: Cat. Nos. WB7001 and WB7002 may be ordered by clicking the Quick Order button located at the top right of any page on our website.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all WesternBreeze™ 洗涤液 (16X) FAQs