Novex™ AP Chemiluminescent Substrate Enhancer (Nitro Block II™) - FAQs

Invitrogen AP化学发光底物（CDP-Star）和Invitrogen ECL学发光底物是非放射性底物，可在免疫印迹膜或点印迹膜上分别用于对碱性磷酸酶（AP）或辣根过氧化物酶（HRP）进行化学发光免疫检测。Invitrogen化学发光底物的检测灵敏度高于沉淀显色底物。利用X射线胶片或成像设备，可使检测达到皮克级别。
Invitrogen AP化学发光底物是由一种二氧杂环丁烷底物CDP-Star的即用型溶液组成，可用于检测碱性磷酸酶。Invitrogen AP化学发光底物增强剂是由Nitro-Block-II的20X溶液组成，在检测硝化纤维素膜上的印迹时，将增强剂与AP化学发光底物混合。增强剂不可用于PVDF膜，否则会产生高背景。膜孵育后20分钟，底物即可发光，并在45-60分钟达到峰强度。发光的最大波长为461–466 nm，并且可持续几小时，有些情况下可持续几天。Invitrogen AP化学发光底物和InvitrogenAP化学发光底物增强剂可作为独立产品（货号WP20002和WP20003）购买，同时，它们也是WesternBreeze化学发光检测试剂盒的组分。
Invitrogen ECL化学发光底物试剂盒由两部分试剂组成，包括鲁米诺溶液和增强剂，可用于检测辣根过氧化物酶。用于印迹膜检测前，将试剂A和试剂B等体积混合。Invitrogen ECL化学发光底物的发光强度在膜孵育后5-30分钟达到峰值，并在数小时内缓慢下降。

可以，您可使用以下货号单独购买这些产品：

•化学发光底物，货号WP20002
•化学发光底物增强剂，货号WP20003

Invitrogen AP Chemiluminescent Substrate (CDP-Star substrate) and Invitrogen ECL Chemiluminescent Substrate are non-radioactive substrates for chemiluminescence-based immunodetection of alkaline phosphatase (AP) or horse radish peroxidase (HRP), respectively, on western blots or dot blots. Invitrogen Chemiluminescent Substrates provide detection sensitivities superior to that of precipitating chromogenic substrates. Low picogram levels of detection can be achieved using either X-ray film or imaging equipment.

Invitrogen AP Chemiluminescent Substrate consists of a ready-to-use solution of CDP-Star substrate, a dioxetane-based substrate for detection of alkaline phosphatase. The Invitrogen AP Chemiluminescent Substrate Enhancer consists of a 20X solution of Nitro-Block-II enhancer that is mixed with the AP Chemiluminescent Substrate when probing blots on nitrocellulose membranes. Do not use the enhancer with PVDF membranes, as it can produce high background. Light emission for this substrate builds rapidly in the first 20 mins after membrane incubation and reaches peak intensity after 45-60 mins. Light emission has a maximal wavelength at 461-466 nm, and continues for several hours and in some cases, for days. Invitrogen AP Chemiluminescent Substrate and Invitrogen AP Chemiluminescent Substrate Enhancer are available as standalone products (Cat. Nos. WP20002 and WP20003) and are also components of the WesternBreeze Chemiluminescent Detection kits.

Invitrogen ECL Chemiluminescent Substrate Reagent Kit is a two-part reagent consisting of a luminol and an enhancer for the detection of horse radish peroxidase. Reagent A and Reagent B are mixed in equal volumes before application to the blot. Light emission for Invitrogen ECL Chemiluminescent Substrate is most intense from 5-30 minutes after membrane incubation and decreases slowly with time over the course of several hours.

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Yes, you can purchase them as standalone products and below are the Cat. Nos.:

- Chemiluminescent Substrate, Cat. No. WP20002
- Chemiluminescent Substrate Enhancer, Cat. No. WP20003

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.