Novex™ HRP Chromogenic Substrate (TMB) - FAQs

查看更多产品信息 Novex™ HRP Chromogenic Substrate (TMB) - FAQs (WP20004)

2 个常见问题解答

Invitrogen AP显色底物（BCIP/NBT）和Invitrogen HRP显色发光底物（TMB）是什么？

Invitrogen AP发光底物（BCIP/NBT）和Invitrogen HRP发光底物（TMB）是即用型的单一成分底物，可在免疫印迹膜或点印迹膜上分别对碱性磷酸酶（AP）或辣根过氧化酶（HRP）进行灵敏的免疫检测。AP底物的灵敏度在100 pg范围内，HRP底物在1 ng范围内。
Invitrogen AP显色底物由5-溴-4-氯-3-吲哚基-1-磷酸盐（BCIP）和硝基四氮唑蓝（NBT）的即用型溶液组成，与碱性磷酸酶反应可形成黑紫色沉淀。该底物可作为独立商品（货号WP20001）购买，也是WesternBreeze显色法检测试剂盒的一个组分。
Invitrogen HRP显色底物由3,3’,5,5’-四甲基联苯胺（TMB）的即用型无毒溶液组成，与辣根过氧化物酶反应可形成蓝色沉淀。该底物可作为独立产品（货号WP20004）购买。

*注意：使用不同的抗原和抗体进行检测，灵敏度也不同。

What are the Invitrogen AP Chromogenic Substrate (BCIP/NBT) and Invitrogen HRP Chromogenic Substrate (TMB)?

- Invitrogen AP Chromogenic Substrate (BCIP/NBT) and Invitrogen HRP Chromogenic Substrate (TMB) are ready-to-use, single component substrates for sensitive immunodetection of alkaline phosphatase (AP) or horse radish peroxidase (HRP), respectively, on western blots or dot blots. The sensitivity of the AP substrate is in the 100 pg range, while the HRP substrate is in the 1 ng range (see Note below).

- Invitrogen AP Chromogenic Substrate consists of a ready-to-use solution of 5-bromo-4-chloro-3-indolyl-1-phosphate (BCIP) and nitroblue tetrazolium (NBT) which forms a black-purple precipitate upon reaction with alkaline phosphatase. It is available as a standalone product (Cat. Nos. WP20001) and is also a component of the WesternBreeze Chromogenic Detection Kits.

- Invitrogen HRP Chromogenic Substrate consists of a ready-to-use non-toxic solution of 3,3',5,5'-tetramethylbenzidine (TMB) which forms a blue precipitate upon reaction with horse radish peroxidase. It is available as a standalone product (Cat. No. WP20004).

Note: Sensitivity may vary depending upon the antigen and antibody used for detection.

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