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查看更多产品信息 XCell4 SureLock™ Midi-Cell - FAQs (WR0100)
32 个常见问题解答
我们推荐使用Invitrogen电源适配器（货号ZA10001），帮助您将导线连接到您的电源上。该适配器随XCell4 SureLock Midi-Cell一起提供。如果使用这种适配器不能解决问题，您可联系电源生产商询问是否有其他方法。
以下是可能原因和解决方案：
- 未从凝胶盒底部去除胶带。应从凝胶盒底部去除胶带。
- 与电源连接不良。使用电压表检测所有连接处的电导率。
- 缓冲液不足。应确保上层缓冲液槽（阴极）中的缓冲液足以浸没上样孔。确保下层缓冲槽中有足够的缓冲液（到达填充线）。
- 上层缓冲液槽中的缓冲液泄漏。若电泳缓冲液高度降低，则表示电泳核心和凝胶盒未正确安置。应重新安装并确保XCell4 SureLock组装体处于锁定位置。
以下是可能原因和解决方案：
- 电压设置过低。按照使用手册的说明设置正确的电压。
- 缓冲液稀释过度。检查缓冲液配方；必要时，重新配制。
- 上层缓冲液槽泄漏。应确保缓冲液核心装置安置稳固。若您使用了缓冲液挡板，应确保将挡板正确安装到核心中。如果核心垫片损坏，应更换新的垫片。应确保XCell4 SureLock组装体处于锁定位置。
我们推荐每次使用完毕后先用温和的洗涤剂清洗装置，再用去离子水冲洗。
XCell4 SureLock Midi-Cell应与电泳专用的外加直流电源一起使用。该电源必须：
•与地面绝缘，使直流输出悬空（安全要求），并且必须配备空载或开路保护
•可在200V下运行
如果您想使用XCell4 SureLock Midi-Cell同时进行4块凝胶电泳，我们推荐使用ZOOM Dual Power Supply。其低电压/高电流输出口可处理4块NuPAGE Bis-Tris凝胶的电压和电流（电压200 V，电流约800 mA）。
PowerEase 90W电源和PowerEase 500电源（2013年12月31日起停产）可用于XCell4 SureLock Midi-Cell，但同时进行2两块凝胶电泳（200V 时，达到2 x ~200 mA最大电流）时将达到电流上限500 mA。若使用这些电源在XCell4 SureLock Midi-Cell中同时进行4块凝胶电泳，电流将超出上限，使电泳速度变慢。电泳耗时大约为原来的两倍。
注意：为了将XCell4 SureLock Midi-Cell的隐蔽式电源线连接到不能与隐蔽式或不可伸缩式电源线一起使用的指定电源，请单独使用Invitrogen电源适配器（货号ZA10001）。
我们不推荐将XCell SureLock Mini-Cell附带的凝胶张力楔用于XCell4 SureLock Midi-Cell，因为专为Mini-Cell设计的凝胶张力楔不适合用于Midi-Cell。
XCell4 SureLock组装体或CAM与凝胶张力楔(gel tension wedge)相似。XCell4 SureLock组装体的主动锁定操作确保每次蛋白质凝胶电泳无故障、无泄漏。将XCell4 SureLock组装体上的操纵杆向前推入锁定位置，即可产生均匀的水平力，从而将凝胶/缓冲液核心装置牢牢固定在下液槽中。XCell4 SureLock组装体上有锁定和非锁定位置的标记，使组装过程简单直观。
若您想使用XCell4 SureLock Midi-Cell进行少于3块凝胶的电泳，可使用XCell4排水挡板。排水挡板应放置于下液槽，替代第二个缓冲液核心。不要向排水挡板中加入任何缓冲液或水。但是，若不小心向排水挡板中加入了水或缓冲液，并不会影响电泳。
XCell4 SureLock Midi-Cell不受酒精的影响，但不能兼容氯化烃（如氯仿）、芳香烃（如甲苯和苯）或丙酮。
以下是我们提供的XCell4 SureLock Midi-Cell替换部件。这些产品只能通过电话订购：
替换部件 - 货号
XCell4 SureLock Midi-Cell盖子 - WR0101
XCell4 SureLock Midi-Cell下液槽 - WR0102
XCell4 SureLock Midi-Cell排水挡板(Displacement Dam) - WR0106
XCell SureLock升级包（包括1个凸轮、1个缓冲液挡板和2个核心）- WR0112
不能。虽然NuPAGE Invitrogen中型凝胶可兼容Bio-Rad Criterion系统（在使用特定适配器的情况下），但不能使用XCell4 SureLock Midi-Cell进行Bio-Rad Criterion凝胶电泳。
注意：我们的小型凝胶与Bio-Rad Mini-PROTEAN装置不兼容。
可以，XCell4 SureLock Midi-Cell是为同时进行1-4块中型凝胶的垂直电泳而设计的。若您想使用XCell4 SureLock Midi-Cell进行少于3块凝胶的电泳，可使用XCell4排水挡板。排水挡板应放置于下液槽，替代第二个缓冲液核心。不要向排水挡板中加入任何缓冲液或水。但是，若不小心向排水挡板中加入了水或缓冲液，并不会影响电泳。
我们的中型凝胶可使用XCell4 SureLock Midi-Cell进行电泳。
XCell4 SureLock Midi-Cell包含下液槽、带电线的盖子、2个缓冲液核心、缓冲液挡板、排水挡板、SureLock组装体和切胶刀。
The XCell4 Displacement Dam is used when fewer than 3 gels are run in the XCell4 SureLock Midi-Cell. The Displacement Dam is placed in the lower buffer chamber instead of a second buffer core. Do not add any buffer or water to the Displacement Dam. However, if water or buffer is accidentally added to the Displacement Dam, it does not affect the electrophoresis run.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Recommended maximum load volumes:
12 + 2-well gel: 45 µL in sample well, 15 µL in marker well
20-well gel: 25 µL
26-well gel: 15 µL
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
You will need a Midi Gel Adapter. The Midi Gel Adapter is a self-adhesive, upper buffer chamber used when running the NuPAGE Invitrogen Midi-Gel in a Bio-Rad Criterion Cell. The Midi Gel Adapter may be filled with up to 60 mL of buffer. The Midi Gel Adapters are available packaged with the Midi-Gels (Invitrogen and NuPAGE Invitrogen Midi Gels with Cat. Nos. that end in "A") and also on their own in packages of 10 (Cat. No. WA0999). Running the NuPAGE Midi-Gels in the XCell4 SureLock Gel Apparatus does not require the use of the Midi Gel Adapter.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Multichannel pipette compatibility: 12+2-well (8- and 12-channel pipette-compatible) and 26-well (8- and 12-channel pipette-compatible).
The 12-channel pipettes work with the 26-well gels. You will need to alternate lanes (e.g., aligns with every two lanes). There will be two extra lanes (for MW markers). The 12-channel pipettes work with the 12+2 well gels as well.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
We recommend using Invitrogen Power Supply Adapters (Cat. No. ZA10001) to help you connect the leads to your power supply. These adapters are supplied along with the XCell4 SureLock Midi-Cell. If these adapters don't work, you may contact the manufacturer of the power supply to see if other options are available.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- Tape has not been removed from the bottom of the cassette. Remove tape from the bottom of the cassette.
- Connection to power supply not complete. Check all connections with a voltmeter for conductance.
- Insufficient buffer level. Make sure the buffer in the Upper Buffer Chamber (cathode) is covering the wells. Be sure there is sufficient buffer (up to the fill line) in the Lower Buffer Chamber.
- Buffer is leaking from the Upper Buffer Chamber. If the level of running buffer drops, it indicates that the electrophoresis core and cassettes are not properly seated. Repeat the assembly and check to ensure the XCell4 SureLock Assembly is in the locked position.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- Voltage is set too low. Set correct voltage as described in the manual.
- Buffers are too dilute. Check buffer recipe; re-make if necessary.
- Upper buffer chamber is leaking. Make sure the buffer core is firmly seated. If you are using the buffer dam, make sure the dam is properly positioned in the core. If the core gasket is damaged, replace with a fresh gasket. Check to ensure that the XCell4 SureLock Assembly is in the locked position.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
We recommend washing the unit with a mild detergent and rinsing with deionized water after each use.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The XCell4 SureLock Midi-Cell should be used with an external DC power supply designed for electrophoresis. This power supply must:
- Be isolated from the ground so that the DC output is floating (safety requirement)and must be equipped with No Load or Open Circuit protection
- Be able to operate at 200 V
If you would like to simultaneously run 4 gels using the XCell4 SureLock Midi-Cell, we recommend using the ZOOM Dual Power Supply. The Low Voltage/High Current outlets can handle the voltage and current of 4 NuPAGE Bis-Tris gels (Voltage 200 V, Current ~800 mA). The PowerEase 90W Power Supply and PowerEase 500 Power Supply (discontinued as of December 31, 2013) can be used with the XCell4 SureLock Midi-Cell but the current limit of 500 mA will be reached with 2 gels (2 x ~200 mA max current at 200V). If 4 gels are run in the XCell4 SureLock Midi-Cell with these power supplies, the current will be maxed out and the run will slow down. It will take approximately twice as long to run the gels. Note: To allow connection of the covered power leads of the XCell4 SureLock Midi-Cell with certain power supplies that are not designed for use with covered or non-retractable power leads, use Invitrogen Power Supply Adapters available separately (Cat. No. ZA10001).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
We do not recommend using the gel tension wedge that is supplied with the XCell SureLock Mini-Cell for the XCell4 SureLock Midi-Cell as it is specifically designed for the Mini-Cell and will not fit correctly in the Midi-Cell.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The XCell4 SureLock Assembly or CAM is similar to a gel tension wedge. The positive locking action of the XCell SureLock Assembly ensures trouble-free, leak-free electrophoresis of protein gels. Move the lever on the XCell SureLock Assembly to a locked position to generate an even horizontal force which seals the gel cassette/Buffer Core assembly firmly into position in the Lower Buffer Chamber. The locked and unlocked positions are marked on the XCell SureLock Assembly for easy, intuitive assembly.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Use the XCell4 Displacement Dam when you wish to run less than 3 gels in the XCell4 SureLock Midi-Cell. The Displacement Dam is placed in the Lower Buffer Chamber instead of a second Buffer Core. Do not add any buffer or water to the Displacement Dam. However, if water or buffer is accidentally added to the Displacement Dam, it does not affect the electrophoresis run.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The XCell4 SureLock Midi-Cell is impervious to alcohol, but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene) or acetone.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are the replacement parts we offer for the XCell4 SureLock Midi-Cell. They can only be ordered by phone:
- XCell4 SureLock Midi-Cell Lid: Cat. No. WR0101
- XCell4 SureLock Midi-Cell Lower Buffer Chamber: Cat. No. WR0102
- XCell4 SureLock Midi-Cell Displacement Dam: Cat. No. WR0106
- XCell SureLock Upgrade kit: Cat. No. WR0112 (contains 1 SureLock Assembly, 1 buffer dam, and 2 cores) Please note that in addition to ordering on the phone by calling Customer Service, this item can also be ordered using the “Quick Order” link (https://www.thermofisher.com/store/quick-order) that can be found at the top right of most pages on our website. The price will display when the item is added to your shopping cart.
Find additional tips, troubleshooting help, and resources within our Protein Gel Electrophoresis Chambers, Power Supplies, and Accessories Support Center.
No. While the NuPAGE Invitrogen Midi Gels are compatible with the Bio-Rad Criterion system (when the specific adapter is used), the Bio-Rad Criterion gels will not work with the XCell4 SureLock Midi-Cell.
Note: Our Mini gels are not compatible with the Bio-Rad Mini-PROTEAN apparatus.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Yes, the XCell4 SureLock Midi-Cell is designed to perform simultaneous vertical electrophoresis of 1-4 midi-gels. Use the XCell4 Displacement Dam when you wish to run less than 3 gels in the XCell4 SureLock Midi-Cell. The Displacement Dam is placed in the Lower Buffer Chamber instead of a second Buffer Core. Do not add any buffer or water to the Displacement Dam. However, if water or buffer is accidentally added to the Displacement Dam, it does not affect the electrophoresis run.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Our Midi gels can be run using the XCell4 SureLock Midi-Cell.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The XCell4 SureLock Midi-Cell includes the lower buffer chamber, lid with cables, two buffer cores, buffer dam, displacement dam, SureLock assembly, and gel knife. For descriptions and images of the various parts, please refer to pages 1-3 of the product manual
(https://assets.thermofisher.com/TFS-Assets/LSG/manuals/xcell4_midicell_man.pdf).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.