Selective, high-resolution fluorescence imaging of mitochondrial Ca2+ concentration.
AuthorsGerencsér AA AA, Adam-Vizi V
JournalCell Calcium
PubMed ID11733937
'We have developed a digital image processing technique based on highpass filtering of microfluorimetric images for selective transmission of fine image details corresponding to mitochondria. This technique enabled the detection of the mitochondrial calcium signals with high selectivity, simultaneously with the cytosolic calcium signal. The validity of this technique was ... More
Imaging mitochondrial calcium signalling with fluorescent probes and single or two photon confocal microscopy.
AuthorsDavidson SM, Duchen MR,
JournalMethods Mol Biol
PubMed ID22057570
The concentration of calcium ions in the mitochondria has a profound impact on mitochondrial function, modulating respiratory activity at physiological concentrations, while causing lethal damage during calcium overload. The ... More
Predicting and avoiding subcellular compartmentalization artifacts arising from acetoxymethyl ester calcium imaging probes. The case of fluo-3 AM and a general account of the phenomenon including a problem avoidance chart.
AuthorsThompson K, Dockery P, Horobin RW,
JournalBiotech Histochem
PubMed ID22984940
Stimulated by difficulties experienced when using fluo-3 AM, we developed a general mechanistic model to aid understanding and practical application of calcium probes applied as acetoxymethyl (AM) esters. Several practical issues previously overlooked or under-emphasized are considered by this model. First, some AM ester probes are ... More