'Although action potentials are typically generated in the axon initial segment (AIS), the timing and pattern of action potentials are thought to depend on inward current originating in somatodendritic compartments. Using two-photon imaging, we show that T- and R-type voltage-gated Ca(2+) channels are colocalized with Na(+) channels in the AIS ... More
In vivo targeting of organic calcium sensors via genetically selected peptides.
AuthorsMarks KM, Rosinov M, Nolan GP
JournalChem Biol
PubMed ID15123264
A library of constrained peptides that form stable folded structures was screened for aptamers that bind with high affinity to the fluorescent dye Texas red. Two selected clones had binding constants to Texas red of 25 and 80 pM as phage and binding had minimal effects on the fluorescence of ... More
Regional differences in hippocampal calcium handling provide a cellular mechanism for limiting plasticity.
AuthorsSimons SB, Escobedo Y, Yasuda R, Dudek SM,
JournalProc Natl Acad Sci U S A
PubMed ID19666491
Although much is known about the mechanisms underlying synaptic plasticity, the cellular mechanisms that negatively regulate plasticity in some brain regions are considerably less studied. One region where neurons do not reliably express long-term potentiation (LTP) is the CA2 subfield of the hippocampus. Given the connection between synaptic plasticity and ... More