Mild mitochondrial uncoupling does not affect mitochondrial biogenesis but downregulates pyruvate carboxylase in adipocytes: role for triglyceride content reduction.
AuthorsDe Pauw A, Demine S, Tejerina S, Dieu M, Delaive E, Kel A, Renard P, Raes M, Arnould T,
JournalAm J Physiol Endocrinol Metab
PubMed ID22354779
'In adipocytes, mitochondrial uncoupling is known to trigger a triglyceride loss comparable with the one induced by TNFa, a proinflammatory cytokine. However, the impact of a mitochondrial uncoupling on the abundance/composition of mitochondria and its connection with triglyceride content in adipocytes is largely unknown. In this work, the effects of ... More
Extrusion of Ca2+ from mouse motor terminal mitochondria via a Na+-Ca2+ exchanger increases post-tetanic evoked release.
AuthorsGarcía-Chacón LE, Nguyen KT, David G, Barrett EF
JournalJ Physiol
PubMed ID16613870
'Mitochondria sequester much of the Ca2+ that enters motor nerve terminals during repetitive stimulation at frequencies exceeding 10-20 Hz. We studied the post-stimulation extrusion of Ca2+ from mitochondria by measuring changes in matrix [Ca2+] with fluorescent indicators loaded into motor terminal mitochondria in the mouse levator auris longus muscle. Trains ... More
Imaging mitochondrial calcium signalling with fluorescent probes and single or two photon confocal microscopy.
AuthorsDavidson SM, Duchen MR,
JournalMethods Mol Biol
PubMed ID22057570
The concentration of calcium ions in the mitochondria has a profound impact on mitochondrial function, modulating respiratory activity at physiological concentrations, while causing lethal damage during calcium overload. The ... More
Predicting and avoiding subcellular compartmentalization artifacts arising from acetoxymethyl ester calcium imaging probes. The case of fluo-3 AM and a general account of the phenomenon including a problem avoidance chart.
AuthorsThompson K, Dockery P, Horobin RW,
JournalBiotech Histochem
PubMed ID22984940
Stimulated by difficulties experienced when using fluo-3 AM, we developed a general mechanistic model to aid understanding and practical application of calcium probes applied as acetoxymethyl (AM) esters. Several practical issues previously overlooked or under-emphasized are considered by this model. First, some AM ester probes are ... More
In vivo targeting of organic calcium sensors via genetically selected peptides.
AuthorsMarks KM, Rosinov M, Nolan GP
JournalChem Biol
PubMed ID15123264
A library of constrained peptides that form stable folded structures was screened for aptamers that bind with high affinity to the fluorescent dye Texas red. Two selected clones had binding constants to Texas red of 25 and 80 pM as phage and binding had minimal effects on the fluorescence of ... More
Different molecular mechanisms involved in spontaneous and oxidative stress-induced mitochondrial fragmentation in TriPeptidyl Peptidase-1 (TPP-1) deficient fibroblasts.
AuthorsVan Beersel G, Tihon E, Demine S, Hamer I, Jadot M, Arnould T,
JournalBiosci Rep
PubMed ID23249249
The Neuronal Ceroid Lipofuscinosis (NCLs) form a group of 8 inherited autosomal recessive diseases characterized by the intralysosomal accumulation of autofluorescent pigments, called ceroids. Recent data suggest that the pathogenesis of NCLs is associated with the appearance of fragmented mitochondria with altered functions. However, even if an impairement in the ... More