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YO-PRO™-1 Iodide (491/509), 1 mL - FAQs

查看更多产品信息 Monomeric Cyanine Nucleic Acid Stains - FAQs (Y3603, P3581, T3605, Y3607, T3602)

4 个常见问题解答

什么是细胞连续凋亡的相对期间时限，我该用什么样的产品去检测不同的凋亡事件？

见本文(https://www.thermofisher.com/content/dam/LifeTech/migration/en/filelibrary/support/bioprobes/bioprobes-65.par.61751.file.dat/bioprobes-65-cellevent.pdf)的表1，在0-4小时的期间，用10 µM喜树碱诱导Jurkat细胞。值得注意的是，这些结果是通过使用单细胞类型和诱导体系研究得到的；对于其他实验体系结果可能不同。凋亡细胞的细胞膜通透性增加，使用YO-PRO-1染料结合碘化丙啶或SYTOX死细胞指示剂能将其与死细胞区分开。其他中期凋亡事件是细胞ROS产物增多（用CellROX reagents，H2DCFDA试剂检测），细胞的pH变化（BCECF, SNARF-1）和钙离子释放（Fluo-4, Fura-2, Indo-1）。任何条件下，没有单个参数能够定义凋亡，因此研究凋亡时最好采用多种参数的方法

What is the suggested working concentration for Monomeric Cyanine Nucleic Acid Stains (Cat. Nos. T3605, P3581, T3602, Y3603, Y3607) for fluorescence microscopy?

For fluorescence microscopy, the working concentration of Monomeric Cyanine Nucleic Acid Stains (Cat. Nos. T3605, P3581, T3602, Y3603, Y3607) is typically 1 - 10 µM. You may need to titrate the dye solution to optimize the working concentration for your sample type and application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the suggested working concentration for Monomeric Cyanine Nucleic Acid Stains (Cat. Nos. T3605, P3581, T3602, Y3603, Y3607) for flow cytometry?

For flow cytometry, the working concentration of Monomeric Cyanine Nucleic Acid Stains (Cat. Nos. T3605, P3581, T3602, Y3603, Y3607) is typically 25 nM - 1 µM. You may need to titrate the dye solution to optimize the working concentration for your sample type and application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the relative time-frame of apoptosis progression, and what products can I use to detect the different apoptotic events?

See Table 1 in this article (http://www.thermofisher.com/content/dam/LifeTech/migration/en/filelibrary/support/bioprobes/bioprobes-65.par.61751.file.dat/bioprobes-65-cellevent.pdf) where a Jurkat model system was induced with 10 µM camptothecin for time periods of 0 to 4 hours. It is important to note that these results were studied using a single cell type and induction system; results may differ for other experimental systems. Increased membrane permeability in apoptotic cells can be discriminated from dead cells using YO-PRO-1 dye in combination with propidium iodide or the SYTOX dead cell indicators. Other mid-apoptotic events are increased ROS production (detected with CellROX reagents, H2DCFDA), changes in cellular pH (BCECF, SNARF-1) and calcium release (Fluo-4, Fura-2, Indo-1). No single parameter defines apoptosis under any condition, so it is best to employ a multi-parametric approach when studying apoptosis.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.