Active caspase-8 translocates into the nucleus of apoptotic cells to inactivate poly(ADP-ribose) polymerase-2.
AuthorsBenchoua A, Couriaud C, Guégan C, Tartier L, Couvert P, Friocourt G, Chelly J, Ménissier-de Murcia J, Onténiente B
JournalJ Biol Chem
PubMed ID12065591
'Caspase-8 is the prototypic initiator of the death domain receptor pathway of apoptosis. Here, we report that caspase-8 not only triggers and amplifies the apoptotic process at cytoplasmic sites but can also act as an executioner at nuclear levels. In a murine model of acute ischemia, caspase-8 is relocated into ... More
Differential effects of deuterium oxide on the fluorescence lifetimes and intensities of dyes with different modes of binding to DNA.
AuthorsSailer BL, Nastasi AJ, Valdez JG, Steinkamp JA, Crissman HA
JournalJ Histochem Cytochem
PubMed ID9016307
'Deuterium oxide (D2O) increases both the fluorescence lifetime and the fluorescence intensity of the intercalating dyes propidium iodide (PI) and ethidium bromide (EB) when bound to nucleic acid structures. We have used spectroscopic analysis coupled with conventional and phase-sensitive flow cytometry to compare the alterations in intensity and lifetime of ... More
Photoreversible DNA condensation using light-responsive surfactants.
AuthorsLe Ny AL, Lee CT
JournalJ Am Chem Soc
PubMed ID16683804
'A means to control DNA compaction with light illumination has been developed using the interaction of DNA with a photoresponsive cationic surfactant. The surfactant undergoes a reversible photoisomerization upon exposure to visible (trans isomer, more hydrophobic) or UV (cis isomer, more hydrophilic) light. As a result, surfactant binding to DNA ... More
Correlating cell cycle with metabolism in single cells: combination of image and metabolic cytometry.
AuthorsKrylov SN, Zhang Z, Chan NW, Arriaga E, Palcic MM, Dovichi NJ
JournalCytometry
PubMed ID10451502
'BACKGROUND: We coin two terms: First, chemical cytometry describes the use of high-sensitivity chemical analysis techniques to study single cells. Second, metabolic cytometry is a form of chemical cytometry that monitors a cascade of biosynthetic and biodegradation products generated in a single cell. In this paper, we describe the combination ... More
Quantitative screening of single copies of human papilloma viral DNA without amplification.
AuthorsLi J, Lee JY, Yeung ES
JournalAnal Chem
PubMed ID16970325
'We describe a novel quantitative viral screening method based on single-molecule detection that does not require amplification. DNA of human papilloma virus (HPV), the major etiological agent of cervical cancer, served as the screening target in this study. Eight 100-nucleotide single-stranded DNA probes were designed complementary to the E6-E7 gene ... More
Some new properties of DNA-YOYO-3 homodimer complexes revealed by electrophoresis and fluorescence lifetime measurements.
AuthorsPopa LM, Winter S, Löber G
JournalBiochem Mol Biol Int
PubMed ID7696991
The DNA bis-intercalator oxazole homodimer (YOYO-3: 1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-methy l- 2,3-dihydro-(benzo-1,3-oxazole)-2-methylidene]-quinolinium tetraiodide) specifically alters the electrophoretic pattern of covalently closed circular (CCC) DNA molecules. Thus, YOYO-3 seems to remove the CCC DNA supercoils and induces the appearance of additional bands by changing the linking number. It also promotes an unusual "star" activity ... More
Quantitative and sequence-specific analysis of DNA-ligand interaction by means of fluorescent intercalator probes.
AuthorsKirschstein O, Sip M, Kittler L
JournalJ Mol Recognit
PubMed ID10867711
A novel method of analysis of double-stranded DNA-ligand interaction is presented. The interaction is monitored by the fluorescence of a DNA bis-intercalator oxazole homodimer YoYo-3. The fluorescence intensity or its decay time reflects the modification of the DNA double helix. The DNA sequence is scanned by hybridization with short oligomers ... More
The positioning and dynamics of origins of replication in the budding yeast nucleus.
AuthorsHeun P, Laroche T, Raghuraman MK, Gasser SM
JournalJ Cell Biol
PubMed ID11266454
We have analyzed the subnuclear position of early- and late-firing origins of DNA replication in intact yeast cells using fluorescence in situ hybridization and green fluorescent protein (GFP)-tagged chromosomal domains. In both cases, origin position was determined with respect to the nuclear envelope, as identified by nuclear pore staining or ... More
Fluorescence enhancement of DNA-bound TO-PRO-3 by incorporation of bromodeoxyuridine to monitor cell cycle kinetics.
AuthorsBeisker W, Weller-Mewe EM, Nüsse M
JournalCytometry
PubMed ID10520203
BACKGROUND:The detection of DNA-incorporated bromodeoxyuridine (BrdUrd) in mammalian cells is a well-known and important technique to study cell cycle. The use of TO-PRO-3 for detection of BrdUrd substitution of DNA by dual-laser flow cytometry has been investigated. METHODS:Fluorescence enhancement of TO-PRO-3 in BrdUrd-labeled cells is registered in combination with the ... More
Influence of M-phase chromatin on the anisotropy of microtubule asters.
AuthorsDogterom M, Félix MA, Guet CC, Leibler S
JournalJ Cell Biol
PubMed ID8601601
In many eukaryotic cells going through M-phase, a bipolar spindle is formed by microtubules nucleated from centrosomes. These microtubules, in addition to being "captured" by kinetochores, may be stabilized by chromatin in two different ways: short-range stabilization effects may affect microtubules in close contact with the chromatin, while long-range stabilization ... More
Corrigendum to 'High-affinity human programmed death-1 ligand-1 variant promotes redirected T cells to kill tumor cells' [Cancer Lett. 447 (2019) 164-173].
AuthorsLiang Z, Li Y, Tian Y, Zhang H, Cai W, Chen A, Chen L, Bao Y, Xiang B, Kan H, Li Y
JournalCancer Lett
PubMed ID30929810
RIPK3 Activation Leads to Cytokine Synthesis that Continues after Loss of Cell Membrane Integrity.
AuthorsOrozco SL, Daniels BP, Yatim N, Messmer MN, Quarato G, Chen-Harris H, Cullen SP, Snyder AG, Ralli-Jain P, Frase S, Tait SWG, Green DR, Albert ML, Oberst A
JournalCell Rep
PubMed ID31461645
'Necroptosis is a form of programmed cell death that is defined by activation of the kinase RIPK3 and subsequent cell membrane permeabilization by the effector MLKL. RIPK3 activation can also promote immune responses via production of cytokines and chemokines. How active cytokine production is coordinated with the terminal process of ... More
Measurement and models accounting for cell death capture hidden variation in compound response.
AuthorsBae SY, Guan N, Yan R, Warner K, Taylor SD, Meyer AS
JournalCell Death Dis
PubMed ID32312951
'Cancer cell sensitivity or resistance is almost universally quantified through a direct or surrogate measure of cell number. However, compound responses can occur through many distinct phenotypic outcomes, including changes in cell growth, apoptosis, and non-apoptotic cell death. These outcomes have divergent effects on the tumor microenvironment, immune response, and ... More
Optimization of Tetrahydroindazoles as Inhibitors of Human Dihydroorotate Dehydrogenase and Evaluation of Their Activity and In Vitro Metabolic Stability.
Authors
JournalJ Med Chem
PubMed ID32212728
Transcriptomic characterization of cancer-testis antigens identifies MAGEA3 as a driver of tumor progression in hepatocellular carcinoma.
Authors
JournalPLoS Genet
PubMed ID34166362
Targeting the ESCRT-III component CHMP2A for noncanonical Caspase-8 activation on autophagosomal membranes.
Authors
JournalCell Death Differ
PubMed ID32807832
Spectral analysis of multiplex Raman probe signatures.
Authors
JournalACS Nano
PubMed ID19206397
SAMHD1 Limits the Efficacy of Forodesine in Leukemia by Protecting Cells against the Cytotoxicity of dGTP.
Authors
JournalCell Rep
PubMed ID32402273
Quantifying cell cycle-dependent drug sensitivities in cancer using a high throughput synchronisation and screening approach.
Authors
JournalEBioMedicine
PubMed ID34049239
Polymer stealthing and mucin-1 retargeting for enhanced pharmacokinetics of an oncolytic vaccinia virus.
Authors
JournalMol Ther Oncolytics
PubMed ID33869742
Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling Couples Proliferation and Cell Death Mechanisms.
Authors
JournalDev Cell
PubMed ID31564612
High-Throughput Cell Death Assays with Single-Cell and Population-Level Analyses Using Real-Time Kinetic Labeling (SPARKL).
Authors
JournalSTAR Protoc
PubMed ID32793892
The pattern recognition receptors dectin-2, mincle, and FcR? impact the dynamics of phagocytosis of Candida, Saccharomyces, Malassezia, and Mucor species.
AuthorsHaider M, Dambuza IM, Asamaphan P, Stappers M, Reid D, Yamasaki S, Brown GD, Gow NAR, Erwig LP
JournalPLoS One
PubMed ID31393930
Phagocytosis is a receptor-mediated process critical to innate immune clearance of pathogens. It proceeds in a regulated sequence of stages: (a) migration of phagocytes towards pathogens, (b) recognition of PAMPs and binding through PRRs, (c) engulfment and internalisation into phagosomes, (d) phagosome maturation, and (e) killing of pathogen or host ... More
EPA and DHA attenuate deoxynivalenol-induced intestinal porcine epithelial cell injury and protect barrier function integrity by inhibiting necroptosis signaling pathway.
AuthorsXiao K, Liu C, Qin Q, Zhang Y, Wang X, Zhang J, Odle J, Lin X, Hu CA, Liu Y
JournalFASEB J
PubMed ID31909535
Deoxynivalenol (DON) is one of the most common mycotoxins that contaminates food or feed and cause intestinal damage. Long-chain n-3 polyunsaturated fatty acids (PUFA) such as EPA and DHA exert beneficial effects on intestinal integrity in animal models and clinical trials. Necroptosis signaling pathway plays a critical role in intestinal ... More