Active caspase-8 translocates into the nucleus of apoptotic cells to inactivate poly(ADP-ribose) polymerase-2.
AuthorsBenchoua A, Couriaud C, Guégan C, Tartier L, Couvert P, Friocourt G, Chelly J, Ménissier-de Murcia J, Onténiente B
JournalJ Biol Chem
PubMed ID12065591
'Caspase-8 is the prototypic initiator of the death domain receptor pathway of apoptosis. Here, we report that caspase-8 not only triggers and amplifies the apoptotic process at cytoplasmic sites but can also act as an executioner at nuclear levels. In a murine model of acute ischemia, caspase-8 is relocated into ... More
P2X purinergic receptor channel expression and function in bovine aortic endothelium.
AuthorsRamirez AN, Kunze DL
JournalAm J Physiol Heart Circ Physiol
PubMed ID12003818
'We examined bovine aortic endothelial cells (BAECs) for the functional expression of P2X receptors, the ATP-gated cation channels. We identified the P2X subtypes present in BAECs using RT-PCR. mRNA was present for only three of seven family members: P2X4, P2X5, and P2X7. We then characterized agonist-activated currents in whole cell ... More
Versatile low-viscosity sieving matrices for nondenaturing DNA separations using capillary array electrophoresis.
AuthorsMadabhushi RS, Vainer M, Dolnik V, Enad S, Barker DL, Harris DW, Mansfield ES
JournalElectrophoresis
PubMed ID9059830
'The high-resolution separation of double-stranded DNA (dsDNA) has important applications in physical mapping strategies and in the analysis of polymerase chain reaction (PCR) products. Although high-resolution separations of dsDNA by capillary electrophoresis (CE) have been reported, pulsed fields were required to achieve complete resolution of DNA fragments beyond 23 kilobase ... More
TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections.
AuthorsBink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Höfler H, Werner M
JournalHistochem Cell Biol
PubMed ID11405057
Confocal laser scanning microscopy (CLSM) is an extensive but reliable tool for assessing the hybridisation signals in fluorescence in situ hybridisation (FISH). Most CLSMs are equipped with an argon-laser and a helium/neon-laser illumination system with excitation wavelengths of 488, 543 and 633 nm. A protocol for an optimal nuclear counterstaining ... More
Fluorescence enhancement of DNA-bound TO-PRO-3 by incorporation of bromodeoxyuridine to monitor cell cycle kinetics.
AuthorsBeisker W, Weller-Mewe EM, Nüsse M
JournalCytometry
PubMed ID10520203
BACKGROUND:The detection of DNA-incorporated bromodeoxyuridine (BrdUrd) in mammalian cells is a well-known and important technique to study cell cycle. The use of TO-PRO-3 for detection of BrdUrd substitution of DNA by dual-laser flow cytometry has been investigated. METHODS:Fluorescence enhancement of TO-PRO-3 in BrdUrd-labeled cells is registered in combination with the ... More
Sensitivity, reproducibility, and accuracy in short tandem repeat genotyping using capillary array electrophoresis.
AuthorsMansfield ES, Vainer M, Enad S, Barker DL, Harris D, Rappaport E, Fortina P
JournalGenome Res
PubMed ID8889558
The Human Genome Initiative has increased significantly the rate at which disease-causing genes are being mapped and sequenced. New cost-effective methods to locate the genes and to characterize disease-causing mutations require robust, reproducible, and accurate protocols for measuring DNA fragment lengths. Capillary array electrophoresis (CAE) offers rapid, high-resolution separations, high ... More
Pan-Cancer Drivers Are Recurrent Transcriptional Regulatory Heterogeneities in Early-Stage Luminal Breast Cancer.
Authors
JournalCancer Res
PubMed ID33531373
Drosophila Sulf1 is required for the termination of intestinal stem cell division during regeneration.
Authors
JournalJ Cell Sci
PubMed ID27888216
Development of the post-natal growth plate requires intraflagellar transport proteins.