Fluorescence-intensity multiplexing: simultaneous seven-marker, two-color immunophenotyping using flow cytometry.
AuthorsBradford JA, Buller G, Suter M, Ignatius M, Beechem JM
JournalCytometry A
PubMed ID15382027
'BACKGROUND: To address the need to resolve multiple biological targets simultaneously, flow cytometers with as many as 10-15 detection channels have been developed. In this study, a new Zenon immunolabeling technology is developed that allows for multiple antigen detection per detection channel using a single fluorophore, through a unique method ... More
A p53 axis regulates B cell receptor-triggered, innate immune system-driven B cell clonal expansion.
Resting mature human B cells undergo a dynamic process of clonal expansion, followed by clonal contraction, during an in vitro response to surrogate C3d-coated Ag and innate immune system cytokines, IL-4 and BAFF. In this study, we explore the mechanism for clonal contraction through following the time- and division-influenced expression ... More