Libraries against libraries for combinatorial selection of replicating antigen-antibody pairs.
AuthorsBowley DR, Jones TM, Burton DR, Lerner RA,
JournalProc Natl Acad Sci U S A
PubMed ID19139405
'Antibodies are among the most highly selective tight-binding ligands for proteins. Because the human genome project has deciphered the proteome, there is an opportunity to use combinatorial antibody libraries to select high-affinity antibodies to every protein encoded by the genome. However, this is a large task because the selection formats ... More
Flow cytometry and FISH to measure the average length of telomeres (flow FISH).
AuthorsBaerlocher GM, Vulto I, de Jong G, Lansdorp PM
JournalNat Protoc
PubMed ID17406480
Telomeres have emerged as crucial cellular elements in aging and various diseases including cancer. To measure the average length of telomere repeats in cells, we describe our protocols that use fluorescent in situ hybridization (FISH) with labeled peptide nucleic acid (PNA) probes specific for telomere repeats in combination with fluorescence ... More
Binding and transfer of human immunodeficiency virus by DC-SIGN+ cells in human rectal mucosa.
AuthorsGurney KB, Elliott J, Nassanian H, Song C, Soilleux E, McGowan I, Anton PA, Lee B
JournalJ Virol
PubMed ID15827191
The role of DC-SIGN on human rectal mucosal dendritic cells is unknown. Using highly purified human rectal mucosal DC-SIGN+ cells and an ultrasensitive real-time reverse transcription-PCR assay to quantify virus binding, we found that HLA-DR+/DC-SIGN+ cells can bind and transfer more virus than the HLA-DR+/DC-SIGN- cells. Greater than 90% of ... More