Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry.
AuthorsFung E, Esposito L, Todd JA, Wicker LS,
JournalNat Protoc
PubMed ID20134434
We describe two modular protocols for immunostaining and multiparameter flow cytometric analysis of major human antigen-presenting cells (APCs; e.g., dendritic cells, monocytes and B lymphocytes) in minimally manipulated whole blood samples. Simultaneous detection of up to eight colors is enabled by careful selection and testing of cell-subset-defining monoclonal antibodies (anchor ... More