Fluorescence-intensity multiplexing: simultaneous seven-marker, two-color immunophenotyping using flow cytometry.
AuthorsBradford JA, Buller G, Suter M, Ignatius M, Beechem JM
JournalCytometry A
PubMed ID15382027
'BACKGROUND: To address the need to resolve multiple biological targets simultaneously, flow cytometers with as many as 10-15 detection channels have been developed. In this study, a new Zenon immunolabeling technology is developed that allows for multiple antigen detection per detection channel using a single fluorophore, through a unique method ... More