Differential localization of the centromere-specific proteins in the major centromeric satellite of Arabidopsis thaliana.
AuthorsShibata F, Murata M
JournalJ Cell Sci
PubMed ID15161939
'The 180 bp family of tandem repetitive sequences, which constitutes the major centromeric satellite in Arabidopsis thaliana, is thought to play important roles in kinetochore assembly. To assess the centromere activities of the 180 bp repeats, we performed indirect fluorescence immunolabeling with antibodies against phosphorylated histone H3 at Ser10, HTR12 ... More
Activity-dependent phosphorylation of tyrosine hydroxylase in dopaminergic neurons of the rat retina.
AuthorsWitkovsky P, Veisenberger E, Haycock JW, Akopian A, Garcia-Espana A, Meller E
JournalJ Neurosci
PubMed ID15115820
'We studied in vivo activity-dependent phosphorylation of tyrosine hydroxylase (TH) in dopaminergic (DA) neurons of the rat retina. TH phosphorylation (TH-P) was evaluated by immunocytochemistry, using antibodies specific for each of three regulated phosphorylation sites. TH synthesis rate was measured by dihydroxyphenylalanine (DOPA) accumulation in the presence of NSD-1015, an ... More
Microtubule-associated [corrected] protein 7 increases the membrane expression of transient receptor potential vanilloid 4 (TRPV4).
AuthorsSuzuki M, Hirao A, Mizuno A
JournalJ Biol Chem
PubMed ID14517216
'The molecular mechanism of the transmission of changes in the shape of the cell surface to ion channels remains obscure. Ca2+ influx induced by cell deformity is inhibited by actin-freezing reagents, suggesting that the actin microfilament couples with an ion channel. Transient receptor potential vanilloid 4 (TRPV4) is a candidate ... More
Multiplex detection of RNA expression in Drosophila embryos.
AuthorsKosman D, Mizutani CM, Lemons D, Cox WG, McGinnis W, Bier E
JournalScience
PubMed ID15297669
We present a fluorescence-based, multiplex in situ hybridization method that permits the simultaneous detection of five differently labeled antisense RNA probes and up to seven differ-ent transcripts in a single Drosophila embryo. We also show that it should be possible to increase the number of detected transcripts substantially with nascent ... More