Administration of Human Protein-C concentrate prevents apoptotic brain cell death after experimental sepsis.
AuthorsMemos N, Betrosian A, Messaris E, Boutsikou M, Kataki A, Chatzigianni E, Nikolopoulou M, Leandros E, Konstadoulakis M,
JournalBrain Res
PubMed ID19368819
Activated Protein C renders anti-apoptotic properties in neurons and endothelial cells. The aim of the present study was to evaluate the in vivo cytoprotective role of Protein C zymogen (PC) administration in septic rat brain. Male Wistar rats (n=60) were subjected to sepsis via Cecal Ligation and Puncture (CLP). Animals ... More
An improved zinc-finger nuclease architecture for highly specific genome editing.
AuthorsMiller JC, Holmes MC, Wang J, Guschin DY, Lee YL, Rupniewski I, Beausejour CM, Waite AJ, Wang NS, Kim KA, Gregory PD, Pabo CO, Rebar EJ
JournalNat Biotechnol
PubMed ID17603475
Genome editing driven by zinc-finger nucleases (ZFNs) yields high gene-modification efficiencies (>10%) by introducing a recombinogenic double-strand break into the targeted gene. The cleavage event is induced using two custom-designed ZFNs that heterodimerize upon binding DNA to form a catalytically active nuclease complex. Using the current ZFN architecture, however, cleavage-competent ... More