Intimin types alpha, beta, and gamma bind to nucleolin with equivalent affinity but lower avidity than to the translocated intimin receptor.
AuthorsSinclair JF, O'Brien AD
JournalJ Biol Chem
PubMed ID15173179
The outer membrane adhesins of enteropathogenic Escherichia coli, Citrobacter rodentium, and enterohemorrhagic E. coli (EHEC) O157:H7 that mediate attach and efface intestinal lesions are classified as intimin alpha, beta, and gamma, respectively. Each of these intimin types binds to its cognate, bacterially encoded receptor (called Tir for translocated intimin receptor) ... More
EphA receptors direct the differentiation of mammalian neural precursor cells through a mitogen-activated protein kinase-dependent pathway.
AuthorsAoki M, Yamashita T, Tohyama M
JournalJ Biol Chem
PubMed ID15145949
'Ephrins are cell surface-associated ligands for Eph receptor tyrosine kinases and are implicated in repulsive axon guidance and cell migration. EphA2, 3, and 4 receptors and one of their cognate ligands, ephrin-A2, are expressed by cells in the subventricular zone and ganglionic eminence of the embryonic day 14.5 telencephalon and ... More
Rapid visualization of microtubules in blood cells and other cell types in marine model organisms.
AuthorsLee KG, Braun A, Chaikhoutdinov I, DeNobile J, Conrad M, Cohen W
JournalBiol Bull
PubMed ID12414579
DNase X is a glycosylphosphatidylinositol-anchored membrane enzyme that provides a barrier to endocytosis-mediated transfer of a foreign gene.
AuthorsShiokawa D, Matsushita T, Shika Y, Shimizu M, Maeda M, Tanuma S
JournalJ Biol Chem
PubMed ID17416904
'DNase X is the first mammalian DNase to be isolated that is homologous to DNase I. In this study, we have examined its function using a novel monoclonal antibody and showed it to be expressed on the cell surface as a glycosylphosphatidylinositolanchored membrane protein. High level expression was observed in ... More
Differential localization of the centromere-specific proteins in the major centromeric satellite of Arabidopsis thaliana.
AuthorsShibata F, Murata M
JournalJ Cell Sci
PubMed ID15161939
'The 180 bp family of tandem repetitive sequences, which constitutes the major centromeric satellite in Arabidopsis thaliana, is thought to play important roles in kinetochore assembly. To assess the centromere activities of the 180 bp repeats, we performed indirect fluorescence immunolabeling with antibodies against phosphorylated histone H3 at Ser10, HTR12 ... More
A seed for Alzheimer amyloid in the brain.
AuthorsHayashi H, Kimura N, Yamaguchi H, Hasegawa K, Yokoseki T, Shibata M, Yamamoto N, Michikawa M, Yoshikawa Y, Terao K, Matsuzaki K, Lemere CA, Selkoe DJ, Naiki H, Yanagisawa K
JournalJ Neurosci
PubMed ID15152051
'A fundamental question about the early pathogenesis of Alzheimer''s disease (AD) concerns how toxic aggregates of amyloid beta protein (Abeta) are formed from its nontoxic soluble form. We hypothesized previously that GM1 ganglioside-bound Abeta (GAbeta) is involved in the process. We now examined this possibility using a novel monoclonal antibody ... More
Cocaine-induced dendritic spine formation in D1 and D2 dopamine receptor-containing medium spiny neurons in nucleus accumbens.
AuthorsLee KW, Kim Y, Kim AM, Helmin K, Nairn AC, Greengard P
JournalProc Natl Acad Sci U S A
PubMed ID16492766
'Psychostimulant-induced alteration of dendritic spines on dopaminoceptive neurons in nucleus accumbens (NAcc) has been hypothesized as an adaptive neuronal response that is linked to long-lasting addictive behaviors. NAcc is largely composed of two distinct subpopulations of medium-sized spiny neurons expressing high levels of either dopamine D1 or D2 receptors. In ... More
Interaction of Hsp90 with the nascent form of the mutant epidermal growth factor receptor EGFRvIII.
AuthorsLavictoire SJ, Parolin DA, Klimowicz AC, Kelly JF, Lorimer IA
JournalJ Biol Chem
PubMed ID12471035
'EGFRvIII is a mutant epidermal growth factor that promotes aggressive growth of glioblastomas. We made a plasmid that directed the expression of an EGFRvIII with three copies of the Flag epitope at its amino terminus. Flag-tagged EGFRvIII was expressed at the same levels as unmodified EGFRvIII, and showed the same ... More
Desmosomal proteins, including desmoglein 3, serve as novel negative markers for epidermal stem cell-containing population of keratinocytes.
AuthorsWan H, Stone MG, Simpson C, Reynolds LE, Marshall JF, Hart IR, Hodivala-Dilke KM, Eady RA
JournalJ Cell Sci
PubMed ID12953062
'No single method has been universally adopted for identifying and isolating epidermal stem/progenitor cells, and the emergence of new markers of stem cell populations is worth exploring. Here we report, for the first time, that clusters of basal keratinocytes at the tips of the rete ridges in human palm, previously ... More
Activity-dependent phosphorylation of tyrosine hydroxylase in dopaminergic neurons of the rat retina.
AuthorsWitkovsky P, Veisenberger E, Haycock JW, Akopian A, Garcia-Espana A, Meller E
JournalJ Neurosci
PubMed ID15115820
'We studied in vivo activity-dependent phosphorylation of tyrosine hydroxylase (TH) in dopaminergic (DA) neurons of the rat retina. TH phosphorylation (TH-P) was evaluated by immunocytochemistry, using antibodies specific for each of three regulated phosphorylation sites. TH synthesis rate was measured by dihydroxyphenylalanine (DOPA) accumulation in the presence of NSD-1015, an ... More
Espins are multifunctional actin cytoskeletal regulatory proteins in the microvilli of chemosensory and mechanosensory cells.
AuthorsSekerková G, Zheng L, Loomis PA, Changyaleket B, Whitlon DS, Mugnaini E, Bartles JR
JournalJ Neurosci
PubMed ID15190118
'Espins are associated with the parallel actin bundles of hair cell stereocilia and are the target of mutations that cause deafness and vestibular dysfunction in mice and humans. Here, we report that espins are also concentrated in the microvilli of a number of other sensory cells: vomeronasal organ sensory neurons, ... More
DNAM-1 and PVR regulate monocyte migration through endothelial junctions.
AuthorsReymond N, Imbert AM, Devilard E, Fabre S, Chabannon C, Xerri L, Farnarier C, Cantoni C, Bottino C, Moretta A, Dubreuil P, Lopez M
JournalJ Exp Med
PubMed ID15136589
'DNAX accessory molecule 1 (DNAM-1; CD226) is a transmembrane glycoprotein involved in T cell and natural killer (NK) cell cytotoxicity. We demonstrated recently that DNAM-1 triggers NK cell-mediated killing of tumor cells upon engagement by its two ligands, poliovirus receptor (PVR; CD155) and Nectin-2 (CD112). In the present paper, we ... More
Importance of TRF1 for functional telomere structure.
AuthorsIwano T, Tachibana M, Reth M, Shinkai Y
JournalJ Biol Chem
PubMed ID14559908
'Telomeres are comprised of telomeric DNA sequences and associated binding molecules. Their structure functions to protect the ends of linear chromosomes and ensure chromosomal stability. One of the mammalian telomere-binding factors, TRF1, localizes telomeres by binding to double-stranded telomeric DNA arrays. Because the overexpression of wild-type and dominant-negative TRF1 induces ... More
Mixed gastric- and intestinal-type metaplasia is formed by cells with dual intestinal and gastric differentiation.
AuthorsNiwa T, Ikehara Y, Nakanishi H, Tanaka H, Inada K, Tsukamoto T, Ichinose M, Tatematsu M
JournalJ Histochem Cytochem
PubMed ID15637340
'We have proposed to divide intestinal metaplasia (IM) into two categories, i.e., a mixed gastric and intestinal (GI) type, and a solely intestinal (I) type, based on the residual gastric phenotype cells. The GI-mixed-type IM can be identified by the presence of both cells with either gastric or intestinal phenotypes ... More
Evaluation of glucose transport and its regulation by insulin in human monocytes using flow cytometry.
AuthorsDimitriadis G, Maratou E, Boutati E, Psarra K, Papasteriades C, Raptis SA
JournalCytometry A
PubMed ID15688355
'BACKGROUND: We investigated the effects of insulin on glucose transport in human monocytes using flow cytometry, a method with several advantages over previously used techniques. We hypothesized that monocytes could be used as tools to study insulin action at the cellular level and facilitate the investigation of mechanisms that lead ... More
Fibroblast growth factor 2 endocytosis in endothelial cells proceed via syndecan-4-dependent activation of Rac1 and a Cdc42-dependent macropinocytic pathway.
AuthorsTkachenko E, Lutgens E, Stan RV, Simons M
JournalJ Cell Sci
PubMed ID15226395
'Full activity of fibroblast growth factors (FGFs) requires their internalization in addition to the interaction with cell surface receptors. Recent studies have suggested that the transmembrane proteoglycan syndecan-4 functions as a FGF2 receptor. In this study we investigated the molecular basis of syndecan endocytosis and its role in FGF2 internalization ... More
Another view of T cell antigen recognition: cooperative engagement of glycolipid antigens by Va14Ja18 natural T(iNKT) cell receptor [corrected].
AuthorsStanic AK, Shashidharamurthy R, Bezbradica JS, Matsuki N, Yoshimura Y, Miyake S, Choi EY, Schell TD, Van Kaer L, Tevethia SS, Roopenian DC, Yamamura T, Joyce S
JournalJ Immunol
PubMed ID14568927
'Va14Ja18 natural T (iNKT) cells rapidly elicit a robust effector response to different glycolipid Ags, with distinct functional outcomes. Biochemical parameters controlling iNKT cell function are partly defined. However, the impact of iNKT cell receptor beta-chain repertoire and how alpha-galactosylceramide (alpha-GalCer) analogues induce distinct functional responses have remained elusive. Using ... More
Chromatin of the Barr body: histone and non-histone proteins associated with or excluded from the inactive X chromosome.
AuthorsChadwick BP, Willard HF
JournalHum Mol Genet
PubMed ID12915472
'The Barr body has long been recognized as the cytological manifestation of the inactive X chromosome (Xi) in interphase nuclei. Despite being known for over 50 years, relatively few components of the Barr body have been identified. In this study, we have screened over 30 histone variants, modified histones and ... More
Matrix protein microarrays for spatially and compositionally controlled microspot thrombosis under laminar flow.
AuthorsOkorie UM, Diamond SL
JournalBiophys J
PubMed ID16905604
'Microarraying allows the spatial and compositional control of surfaces, typically for the purpose of binding reactions. Collagen and/or von Willebrand Factor (vWF) in 5% glycerol was contact printed onto glass slides to create defined microspots (176-microm diameter) of adsorbed protein without sample dehydration. The arrays were mounted on flow chambers ... More
ICln, a novel integrin alphaIIbbeta3-associated protein, functionally regulates platelet activation.
AuthorsLarkin D, Murphy D, Reilly DF, Cahill M, Sattler E, Harriott P, Cahill DJ, Moran N
JournalJ Biol Chem
PubMed ID15075326
'A critical role for the conserved alpha-integrin cytoplasmic motif, KVGFFKR, is recognized in the regulation of activation of the platelet integrin alpha(IIb)beta(3). To understand the molecular mechanisms of this regulation, we sought to determine the nature of the protein interactions with this cytoplasmic motif. We used a tagged synthetic peptide, ... More
Interaction codes within the family of mammalian Phox and Bem1p domain-containing proteins.
AuthorsLamark T, Perander M, Outzen H, Kristiansen K, Øvervatn A, Michaelsen E, Bjørkøy G, Johansen T,
JournalJ Biol Chem
PubMed ID12813044
'The Phox and Bem1p (PB1) domain constitutes a recently recognized protein-protein interaction domain found in the atypical protein kinase C (aPKC) isoenzymes, lambda/iota- and zeta PKC; members of mitogen-activated protein kinase (MAPK) modules like MEK5, MEKK2, and MEKK3; and in several scaffold proteins involved in cellular signaling. Among the last ... More
Tumor cell alpha3beta1 integrin and vascular laminin-5 mediate pulmonary arrest and metastasis.
AuthorsWang H, Fu W, Im JH, Zhou Z, Santoro SA, Iyer V, DiPersio CM, Yu QC, Quaranta V, Al-Mehdi A, Muschel RJ
JournalJ Cell Biol
PubMed ID15024036
'Arrest of circulating tumor cells in distant organs is required for hematogenous metastasis, but the tumor cell surface molecules responsible have not been identified. Here, we show that the tumor cell alpha3beta1 integrin makes an important contribution to arrest in the lung and to early colony formation. These analyses indicated ... More
Transcriptional regulation of P450scc gene expression in the embryonic rodent nervous system.
AuthorsHammer F, Compagnone NA, Vigne JL, Bair SR, Mellon SH
JournalEndocrinology
PubMed ID14576192
'Steroid hormones are synthesized in adrenals, gonads, placenta, and the central and peripheral nervous systems (neurosteroids). Neurosteroidogenesis, like conventional steroidogenesis, begins with the conversion of cholesterol to pregnenolone, catalyzed by mitochondrial P450 side-chain cleavage enzyme (P450scc). Transcription of the P450scc gene in the adrenals and gonads requires steroidogenic factor-1, which ... More
Internalization of exogenously added memapsin 2 (beta-secretase) ectodomain by cells is mediated by amyloid precursor protein.
'Memapsin 2 (beta-secretase) is the protease that initiates cleavage of amyloid precursor protein (APP) leading to the production of amyloid-beta (Abeta) peptide and the onset of Alzheimer''s disease. Both APP and memapsin 2 are Type I transmembrane proteins and are endocytosed into endosomes where APP is cleaved by memapsin 2. ... More
Expression of the integrin subunit alpha8 in murine lung development.
AuthorsWagner TE, Frevert CW, Herzog EL, Schnapp LM
JournalJ Histochem Cytochem
PubMed ID14500699
'The complex interplay between cells and extracellular matrix (ECM) proteins is critical for lung development. Integrins are key modulators of this interaction. The integrin subunit alpha 8 associates with the beta(1)-subunit to form an RGD-binding integrin. We previously showed that, in adult lung, alpha 8 is expressed in contractile interstitial ... More
Molecular properties of adult mouse gastric and intestinal epithelial progenitors in their niches.
AuthorsGiannakis M, Stappenbeck TS, Mills JC, Leip DG, Lovett M, Clifton SW, Ippolito JE, Glasscock JI, Arumugam M, Brent MR, Gordon JI
JournalJ Biol Chem
PubMed ID16464855
'We have sequenced 36,641 expressed sequence tags from laser capture microdissected adult mouse gastric and small intestinal epithelial progenitors, obtaining 4031 and 3324 unique transcripts, respectively. Using Gene Ontology (GO) terms, each data set was compared with cDNA libraries from intact adult stomach and small intestine. Genes in GO categories ... More
Microtubule-associated [corrected] protein 7 increases the membrane expression of transient receptor potential vanilloid 4 (TRPV4).
AuthorsSuzuki M, Hirao A, Mizuno A
JournalJ Biol Chem
PubMed ID14517216
'The molecular mechanism of the transmission of changes in the shape of the cell surface to ion channels remains obscure. Ca2+ influx induced by cell deformity is inhibited by actin-freezing reagents, suggesting that the actin microfilament couples with an ion channel. Transient receptor potential vanilloid 4 (TRPV4) is a candidate ... More
Inhibition of respiratory syncytial virus infection with intranasal siRNA nanoparticles targeting the viral NS1 gene.
AuthorsZhang W, Yang H, Kong X, Mohapatra S, San Juan-Vergara H, Hellermann G, Behera S, Singam R, Lockey RF, Mohapatra SS
JournalNat Med
PubMed ID15619625
'Respiratory syncytial virus (RSV) infection is one of the major causes of respiratory tract infection for which no vaccine or antiviral treatment is available. The RSV NS1 protein seems to antagonize the host interferon (IFN) response; however, its mechanism is unknown. Here, we used a plasmid-borne small interfering RNA targeting ... More
Adenosine 5'-triphosphate-sensitive potassium channel-mediated blood-brain tumor barrier permeability increase in a rat brain tumor model.
AuthorsNingaraj NS, Rao MK, Black KL
JournalCancer Res
PubMed ID14695207
'Brain tumor microvessels/capillaries limit drug delivery to tumors by forming a blood-brain tumor barrier (BTB). The BTB overexpresses ATP-sensitive potassium (K(ATP)) channels that are barely detectable in normal brain capillaries, and which were targeted for BTB permeability modulation. In a rat brain tumor model, we infused minoxidil sulfate (MS), a ... More
Cytoskeletal events preceding polar body formation in activated Spisula eggs.
AuthorsPielak RM, Gaysinskaya VA, Cohen WD
JournalBiol Bull
PubMed ID14583524
Outbreak of Burkholderia cepacia bacteremia in a pediatric hospital due to contamination of lipid emulsion stoppers.
AuthorsDoit C, Loukil C, Simon AM, Ferroni A, Fontan JE, Bonacorsi S, Bidet P, Jarlier V, Aujard Y, Beaufils F, Bingen E
JournalJ Clin Microbiol
PubMed ID15131197
We describe a 7-month outbreak of nosocomial Burkholderia cepacia bacteremia involving eight children in a pediatric hospital and the results of epidemiological investigations. A B. cepacia strain genotypically identical to the blood isolates was recovered from the upper surface of capped rubber stoppers of bottles of a commercial lipid emulsion ... More
Cutting edge: influence of the TCR V beta domain on the avidity of CD1d:alpha-galactosylceramide binding by invariant V alpha 14 NKT cells.
AuthorsSchümann J, Voyle RB, Wei BY, MacDonald HR
JournalJ Immunol
PubMed ID12794105
CD1d tetramers loaded with alpha-galactosylceramide (alpha-GalCer) bind selectively to mouse invariant Valpha14 (Valpha14i) NKT cells and their human counterparts. Whereas tetramer binding strictly depends on the expression of a Valpha14-Jalpha18 chain in murine NKT cells, the associated beta-chain (typically expressing Vbeta8.2 or Vbeta7) appears not to influence tetramer binding. In ... More
Mammalian Fat1 cadherin regulates actin dynamics and cell-cell contact.
AuthorsTanoue T, Takeichi M
JournalJ Cell Biol
PubMed ID15148305
Fat cadherins form a distinct subfamily of the cadherin gene superfamily, and are featured by their unusually large extracellular domain. In this work, we investigated the function of a mammalian Fat cadherin. Fat1 was localized at filopodial tips, lamellipodial edges, and cell-cell boundaries, overlapping with dynamic actin structures. RNA interference-mediated ... More
Alternative inclusion of fibroblast growth factor receptor 2 exon IIIc in Dunning prostate tumors reveals unexpected epithelial mesenchymal plasticity.
AuthorsOltean S, Sorg BS, Albrecht T, Bonano VI, Brazas RM, Dewhirst MW, Garcia-Blanco MA
JournalProc Natl Acad Sci U S A
PubMed ID16963563
In epithelial cells, alternative splicing of fibroblast growth factor receptor 2 (FGFR2) transcripts leads to the expression of the FGFR2(IIIb) isoform, whereas in mesenchymal cells, the same process results in the synthesis of FGFR2(IIIc). Expression of the FGFR2(IIIc) isoform during prostate tumor progression suggests a disruption of the epithelial character ... More
Asymmetrical beta-actin mRNA translation in growth cones mediates attractive turning to netrin-1.
AuthorsLeung KM, van Horck FP, Lin AC, Allison R, Standart N, Holt CE
JournalNat Neurosci
PubMed ID16980963
Local protein synthesis regulates the turning of growth cones to guidance cues, yet little is known about which proteins are synthesized or how they contribute to directional steering. Here we show that beta-actin mRNA resides in Xenopus laevis retinal growth cones where it binds to the RNA-binding protein Vg1RBP. Netrin-1 ... More
Human mesenchymal stem cells form Purkinje fibers in fetal sheep heart.
AuthorsAirey JA, Almeida-Porada G, Colletti EJ, Porada CD, Chamberlain J, Movsesian M, Sutko JL, Zanjani ED
JournalCirculation
PubMed ID15023887
BACKGROUND: We have investigated the usefulness of a model of cardiac development in a large mammal, sheep, for studies of engraftment of human stem cells in the heart. METHODS AND RESULTS: Adult and fetal human mesenchymal stem cells were injected intraperitoneally into sheep fetuses in utero. Hearts at late fetal ... More
Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes.
AuthorsBache KG, Brech A, Mehlum A, Stenmark H
JournalJ Cell Biol
PubMed ID12900395
Hrs and the endosomal sorting complexes required for transport, ESCRT-I, -II, and -III, are involved in the endosomal sorting of membrane proteins into multivesicular bodies and lysosomes or vacuoles. The ESCRT complexes are also required for formation of intraluminal endosomal vesicles and for budding of certain enveloped RNA viruses such ... More
Molecular characterization of Rab11 interactions with members of the family of Rab11-interacting proteins.
AuthorsJunutula JR, Schonteich E, Wilson GM, Peden AA, Scheller RH, Prekeris R
JournalJ Biol Chem
PubMed ID15173169
The Rab11 subfamily of GTPases plays an important role in vesicle trafficking from endosomes to the plasma membrane. At least six Rab11 effectors (family of Rab11-interacting proteins (FIPs)) have been shown to interact with Rab11 and are hypothesized to regulate various membrane trafficking pathways such as transferrin recycling, cytokinesis, and ... More
Complement receptor type 1 (CD35) mediates inhibitory signals in human B lymphocytes.
AuthorsJózsi M, Prechl J, Bajtay Z, Erdei A
JournalJ Immunol
PubMed ID11884446
The complement system---particularly component C3---has been demonstrated to be a key link between innate and adaptive immunity. The trimolecular complex of complement receptor type 2 (CR2), CD19, and CD81 is known to promote B cell activation when coligated with the B cell Ag receptor. In the present study, we aimed ... More
Physiological calcium concentrations regulate calmodulin binding and catalysis of adenylyl cyclase exotoxins.
AuthorsShen Y, Lee YS, Soelaiman S, Bergson P, Lu D, Chen A, Beckingham K, Grabarek Z, Mrksich M, Tang WJ
JournalEMBO J
PubMed ID12485993
Edema factor (EF) and CyaA are calmodulin (CaM)-activated adenylyl cyclase exotoxins involved in the pathogenesis of anthrax and whooping cough, respectively. Using spectroscopic, enzyme kinetic and surface plasmon resonance spectroscopy analyses, we show that low Ca(2+) concentrations increase the affinity of CaM for EF and CyaA causing their activation, but ... More
An efflux transporter of silicon in rice.
AuthorsMa JF, Yamaji N, Mitani N, Tamai K, Konishi S, Fujiwara T, Katsuhara M, Yano M
JournalNature
PubMed ID17625566
Silicon is an important nutrient for the optimal growth and sustainable production of rice. Rice accumulates up to 10% silicon in the shoot, and this high accumulation is required to protect the plant from multiple abiotic and biotic stresses. A gene, Lsi1, that encodes a silicon influx transporter has been ... More
Monocyte recruitment into the lungs in pneumococcal pneumonia.
AuthorsGoto Y, Hogg JC, Whalen B, Shih CH, Ishii H, Van Eeden SF
JournalAm J Respir Cell Mol Biol
PubMed ID14578212
The recruitment of monocytes into the alveolar spaces is crucial for clearing infections and resolving the inflammatory response. We have previously reported the effect of acute pneumonia on monocyte transport through the bone marrow, and the present study concerns their clearance from the blood and migration into the lung airspaces. ... More
Application of antigen retrieval by heating for double-label fluorescent immunohistochemistry with identical species-derived primary antibodies.
AuthorsIno H
JournalJ Histochem Cytochem
PubMed ID15314088
Double-label fluorescent immunohistochemistry (IHC) is frequently used to identify cellular and subcellular co-localization of independent antigens. In general, primary antibodies for double labeling should be derived from independent species. However, such convenient pairs of antibodies are not always available. To overcome this problem, several methods for double labeling with primary ... More
Bcl-2 on the endoplasmic reticulum regulates Bax activity by binding to BH3-only proteins.
AuthorsThomenius MJ, Wang NS, Reineks EZ, Wang Z, Distelhorst CW
JournalJ Biol Chem
PubMed ID12477729
Bcl-2 family members have been shown to be key mediators of apoptosis as either pro- or anti-apoptotic factors. It is thought that both classes of Bcl-2 family members act at the level of the mitochondria to regulate apoptosis, although the founding anti-apoptotic family member, Bcl-2 is localized to the endoplasmic ... More
Somitic origin of limb muscle satellite and side population cells.
AuthorsSchienda J, Engleka KA, Jun S, Hansen MS, Epstein JA, Tabin CJ, Kunkel LM, Kardon G
JournalProc Natl Acad Sci U S A
PubMed ID16418263
Repair of mature skeletal muscle is mediated by adult muscle progenitors. Satellite cells have long been recognized as playing a major role in muscle repair, whereas side population (SP) cells have more recently been identified as contributing to this process. The developmental source of these two progenitor populations has been ... More
Properties of phosphoenolpyruvate mutase, the first enzyme in the aminoethylphosphonate biosynthetic pathway in Trypanosoma cruzi.
AuthorsSarkar M, Hamilton CJ, Fairlamb AH
JournalJ Biol Chem
PubMed ID12672809
Phosphoenolpyruvate (PEP) mutase catalyzes the conversion of phosphoenolpyruvate to phosphonopyruvate, the initial step in the formation of many naturally occurring phosphonate compounds. The phosphonate compound 2-aminoethylphosphonate is present as a component of complex carbohydrates on the surface membrane of many trypanosomatids including glycosylinositolphospholipids of Trypanosoma cruzi. Using partial sequence information ... More
Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging.
AuthorsHua W, Sheff D, Toomre D, Mellman I
JournalJ Cell Biol
PubMed ID16567501
Although epithelial cells are known to exhibit a polarized distribution of membrane components, the pathways responsible for delivering membrane proteins to their appropriate domains remain unclear. Using an optimized approach to three-dimensional live cell imaging, we have visualized the transport of newly synthesized apical and basolateral membrane proteins in fully ... More
Validation of a novel ultra-short immunolabeling method for high-quality mRNA preservation in laser microdissection and real-time reverse transcriptase-polymerase chain reaction.
Authorsvon Smolinski D, Blessenohl M, Neubauer C, Kalies K, Gebert A
JournalJ Mol Diagn
PubMed ID16645212
Laser microdissection allows isolation of tiny samples from tissue sections for analysis of gene expression by real-time quantitative polymerase chain reaction (PCR). Although immunohistochemical labeling is often required to identify target structures, it drastically degrades mRNA so that shortened protocols are needed. Here, we present a novel method that allows ... More
Caspase-cleavage of tau is an early event in Alzheimer disease tangle pathology.
Neurofibrillary tangles (NFTs) are composed of abnormal aggregates of the cytoskeletal protein tau. Together with amyloid beta (Abeta) plaques and neuronal and synaptic loss, NFTs constitute the primary pathological hallmarks of Alzheimer disease (AD). Recent evidence also suggests that caspases are activated early in the progression of AD and may ... More
SLC26A7: a basolateral Cl-/HCO3- exchanger specific to intercalated cells of the outer medullary collecting duct.
AuthorsPetrovic S, Barone S, Xu J, Conforti L, Ma L, Kujala M, Kere J, Soleimani M
JournalAm J Physiol Renal Physiol
PubMed ID12965893
The outer medullary collecting duct (OMCD) plays an important role in bicarbonate reabsorption and acid-base regulation. An apical V-type H+-ATPase and a basolateral Cl-/HCO3- exchanger, located in intercalated cells of OMCD, mediate the bicarbonate reabsorption. Here we report the identification of a new basolateral Cl-/HCO3- exchanger in OMCD intercalated cells ... More
Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP.
AuthorsUeda J, Tachibana M, Ikura T, Shinkai Y
JournalJ Biol Chem
PubMed ID16702210
G9a is a SET-domain mammalian histone methyltransferase responsible for mono- and dimethylation of lysine 9 in histone H3 (H3K9) at euchromatic regions. Recently we reported that G9a forms a stoichiometric heteromeric complex with another SET-domain-containing molecule, GLP/Eu-HMTase1. Although G9a and GLP can independently methylate H3K9 in vitro, G9a/GLP heteromeric formation ... More
The low density lipoprotein receptor-related protein modulates protease activity in the brain by mediating the cellular internalization of both neuroserpin and neuroserpin-tissue-type plasminogen activator complexes.
AuthorsMakarova A, Mikhailenko I, Bugge TH, List K, Lawrence DA, Strickland DK
JournalJ Biol Chem
PubMed ID14522960
Proteases contribute to a variety of processes in the brain; consequently, their activity is carefully regulated by protease inhibitors, such as neuroserpin. This inhibitor is thought to be secreted by axons at synaptic regions where it controls tissue-type plasminogen activator (tPA) activity. Mechanisms regulating neuroserpin are not known, and the ... More
Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes.
AuthorsTirouvanziam R, Davidson CJ, Lipsick JS, Herzenberg LA
JournalProc Natl Acad Sci U S A
PubMed ID14976247
Drosophila is a powerful model for molecular studies of hematopoiesis and innate immunity. However, its use for functional cellular studies remains hampered by the lack of single-cell assays for hemocytes (blood cells). Here we introduce a generic method combining fluorescence-activated cell sorting and nonantibody probes that enables the selective gating ... More
Dual roles of PspC, a surface protein of Streptococcus pneumoniae, in binding human secretory IgA and factor H.
AuthorsDave S, Carmicle S, Hammerschmidt S, Pangburn MK, McDaniel LS
JournalJ Immunol
PubMed ID15210807
Streptococcus pneumoniae, also known as the pneumococcus, contains several surface proteins that along with the polysaccharide capsule function in antiphagocytic activities and evasion of the host immune system. These pneumococcal proteins interact with the host immune system in various ways and possess a wide range of biological activities that suggests ... More
Binding and transfer of human immunodeficiency virus by DC-SIGN+ cells in human rectal mucosa.
AuthorsGurney KB, Elliott J, Nassanian H, Song C, Soilleux E, McGowan I, Anton PA, Lee B
JournalJ Virol
PubMed ID15827191
The role of DC-SIGN on human rectal mucosal dendritic cells is unknown. Using highly purified human rectal mucosal DC-SIGN+ cells and an ultrasensitive real-time reverse transcription-PCR assay to quantify virus binding, we found that HLA-DR+/DC-SIGN+ cells can bind and transfer more virus than the HLA-DR+/DC-SIGN- cells. Greater than 90% of ... More
Interactions between synaptic vesicle fusion proteins explored by atomic force microscopy.
AuthorsYersin A, Hirling H, Steiner P, Magnin S, Regazzi R, Hüni B, Huguenot P, De los Rios P, Dietler G, Catsicas S, Kasas S
JournalProc Natl Acad Sci U S A
PubMed ID12853568
Measuring the biophysical properties of macromolecular complexes at work is a major challenge of modern biology. The protein complex composed of vesicle-associated membrane protein 2, synaptosomal-associated protein of 25 kDa, and syntaxin 1 [soluble N-ethyl-maleimide-sensitive factor attachment protein receptor (SNARE) complex] is essential for docking and fusion of neurotransmitter-filled synaptic ... More
The combination of the Zenon labeling technique and microscopic image analysis to study cell populations in normal and psoriatic epidermis.
Authorsvan Duijnhoven MW, van de Kerkhof PC, Pasch MC, Muys L, van Erp PE
JournalJ Cutan Pathol
PubMed ID15701083
BACKGROUND: In order to better characterize epidermal cell populations in psoriatic vs. normal skin, fluorescent immunohistochemical techniques were extended with a new labeling technique. The Zenon technique conjugates primary antibodies rapidly and quantitatively after which they are used in the same manner as covalently labeled primary antibodies. Digital microscopic images ... More
Distinct endosomal compartments in early trafficking of low density lipoprotein-derived cholesterol.
AuthorsSugii S, Reid PC, Ohgami N, Du H, Chang TY
JournalJ Biol Chem
PubMed ID12721287
We previously studied the early trafficking of low density lipoprotein (LDL)-derived cholesterol in mutant Chinese hamster ovary cells defective in Niemann-Pick type C1 (NPC1) using cyclodextrin (CD) to monitor the arrival of cholesterol from the cell interior to the plasma membrane (PM) (Cruz, J. C., Sugii, S., Yu, C., and ... More
Actinobacillus actinomycetemcomitans cytolethal distending toxin (Cdt): evidence that the holotoxin is composed of three subunits: CdtA, CdtB, and CdtC.
AuthorsShenker BJ, Besack D, McKay T, Pankoski L, Zekavat A, Demuth DR
JournalJ Immunol
PubMed ID14688349
We have shown the Actinobacillus actinomycetemcomitans produces an immunosuppressive factor encoded by the cytolethal distending toxin (cdt)B gene, which is homologous to a family of Cdts expressed by several Gram-negative bacteria. We now report that the capacity for CdtB to induce G(2) arrest in Jurkat cells is greater in the ... More
Proinflammatory phenotype of coronary arteries promotes endothelial apoptosis in aging.
AuthorsCsiszar A, Ungvari Z, Koller A, Edwards JG, Kaley G
JournalPhysiol Genomics
PubMed ID15020720
Previously we demonstrated that aging in coronary arteries is associated with proinflammatory phenotypic changes and decreased NO bioavailability, which, we hypothesized, promotes vascular disease by enhancing endothelial apoptosis. To test this hypothesis we characterized proapoptotic alterations in the phenotype of coronary arteries of aged (26 mo old) and young (3 ... More
Regulation of angiotensin II type 1A receptor intracellular retention, degradation, and recycling by Rab5, Rab7, and Rab11 GTPases.
AuthorsDale LB, Seachrist JL, Babwah AV, Ferguson SS
JournalJ Biol Chem
PubMed ID14711821
Previous studies have demonstrated that the interaction of the angiotensin II type 1A receptor (AT(1A)R) carboxyl-terminal tail with Rab5a may modulate Rab5a activity, leading to the homotypic fusion of endocytic vesicles. Therefore, we have investigated whether AT(1A)R/Rab5a interactions mediate the retention of AT(1A)R.beta-arrestin complexes in early endosomes and whether the ... More
Adaptor protein complex 1 mediates the transport of lysosomal proteins from a Golgi-like organelle to peripheral vacuoles in the primitive eukaryote Giardia lamblia.
AuthorsTouz MC, Kulakova L, Nash TE
JournalMol Biol Cell
PubMed ID15107467
Giardia lamblia is an early branching protist that possesses peripheral vacuoles (PVs) with characteristics of lysosome-like organelles, located underneath the plasma membrane. In more evolved cells, lysosomal protein trafficking is achieved by cargo recognition involving adaptor protein (AP) complexes that recognize specific amino acid sequences (tyrosine and/or dileucine motifs) within ... More
Nrf1 is targeted to the endoplasmic reticulum membrane by an N-terminal transmembrane domain. Inhibition of nuclear translocation and transacting function.
AuthorsWang W, Chan JY
JournalJ Biol Chem
PubMed ID16687406
Expression of antioxidant and phase 2 xenobiotic metabolizing enzyme genes is regulated through cis-acting sequences known as antioxidant response elements. Transcriptional activation through the antioxidant response elements involves members of the CNC (Cap 'n' Collar) family of basic leucine zipper proteins including Nrf1 and Nrf2. Nrf2 activity is regulated by ... More
Active and passive mechanisms drive secretory granule biogenesis during differentiation of the intestinal parasite Giardia lamblia.
AuthorsGottig N, Elías EV, Quiroga R, Nores MJ, Solari AJ, Touz MC, Luján HD
JournalJ Biol Chem
PubMed ID16611634
The parasitic protozoan Giardia lamblia undergoes important changes to survive outside the intestine of its host by differentiating into infective cysts. During encystation, three cyst wall proteins (CWPs) are specifically expressed and concentrated within encystation-specific secretory vesicles (ESVs). ESVs are electron-dense secretory granules that transport CWPs before exocytosis and extracellular ... More
Covalent and noncovalent modifications induce allosteric binding behavior in a monoclonal antibody.
AuthorsBlake RC, Li X, Yu H, Blake DA
JournalBiochemistry
PubMed ID17279622
Detailed equilibrium binding studies were conducted on a monoclonal antibody (8A11) directed against UO22+ complexed with 2,9-dicarboxy-1,10-phenanthroline (DCP-UO22+). Covalent modification of 8A11 with amine-reactive derivatives of Cy5 or Alexa 488 altered the binding curves obtained with DCP-UO22+ from hyperbolic to sigmoidal, the latter characterized by Hill coefficients of 1.5-1.6. Binding ... More
Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a.
AuthorsRastaldi MP, Armelloni S, Berra S, Li M, Pesaresi M, Poczewski H, Langer B, Kerjaschki D, Henger A, Blattner SM, Kretzler M, Wanke R, D'Amico G
JournalAm J Pathol
PubMed ID12937130
Several recent studies have focused on similarities between glomerular podocytes and neurons because the two cells share a specialized cytoskeletal organization and several expression-restricted proteins, such as nephrin and synaptopodin. In neurons, the small guanosine triphosphatase Rab3A and its effector rabphilin-3A form a complex required for the correct docking of ... More
Effects of overexpression of membrane-bound transferrin-like protein (MTf) on chondrogenic differentiation in Vitro.
AuthorsSuardita K, Fujimoto K, Oda R, Shimazu A, Miyazaki K, Kawamoto T, Kato Y
JournalJ Biol Chem
PubMed ID12374788
Membrane-bound transferrin-like protein (MTf) is expressed in parallel with the expression of cartilage-characteristic genes during differentiation of chondrocytes, and the MTf level is much higher in cartilage than in other tissues. To investigate the role of MTf in cartilage, we examined the effects of growth factors on MTf expression in ... More
Laminin 5 deposition regulates keratinocyte polarization and persistent migration.
AuthorsFrank DE, Carter WG
JournalJ Cell Sci
PubMed ID14996912
Repair of wounded epidermis requires both keratinocyte migration and deposition of laminin 5 over exposed dermal collagen. To understand the coupling between leading cell migration and laminin 5 deposition, we developed a novel migration assay using time-lapse microscopy. We demonstrate that in migrating, human keratinocytes the deposition of laminin 5 ... More
Matrix metalloproteinases expressed by astrocytes mediate extracellular amyloid-beta peptide catabolism.
AuthorsYin KJ, Cirrito JR, Yan P, Hu X, Xiao Q, Pan X, Bateman R, Song H, Hsu FF, Turk J, Xu J, Hsu CY, Mills JC, Holtzman DM, Lee JM
JournalJ Neurosci
PubMed ID17065436
It has been postulated that the development of amyloid plaques in Alzheimer's disease (AD) may result from an imbalance between the generation and clearance of the amyloid-beta peptide (Abeta). Although familial AD appears to be caused by Abeta overproduction, sporadic AD (the most prevalent form) may result from impairment in ... More
Expression of the tumor suppressor protein 14-3-3 sigma is down-regulated in invasive transitional cell carcinomas of the urinary bladder undergoing epithelial-to-mesenchymal transition.
AuthorsMoreira JM, Gromov P, Celis JE
JournalMol Cell Proteomics
PubMed ID14736829
The 14-3-3 proteins constitute a family of abundant, highly conserved and broadly expressed acidic polypeptides that are involved in the regulation of various cellular processes such as cell-cycle progression, cell growth, differentiation, and apoptosis. One member of this family, the 14-3-3 isoform sigma, is expressed only in epithelial cells and ... More
Plasma membrane topology of syncytial domains of herpes simplex virus type 1 glycoprotein K (gK): the UL20 protein enables cell surface localization of gK but not gK-mediated cell-to-cell fusion.
AuthorsFoster TP, Alvarez X, Kousoulas KG
JournalJ Virol
PubMed ID12477855
Most spontaneously occurring mutations that cause extensive herpes simplex virus type 1 (HSV-1)-induced cell fusion are single amino acid changes within glycoprotein K (gK). Despite the strong genetic association of gK with virus-induced cell fusion, its direct involvement in cellular membrane fusion has been controversial, largely due to previously unsuccessful ... More
CD4 receptor localized to non-raft membrane microdomains supports HIV-1 entry. Identification of a novel raft localization marker in CD4.
AuthorsPopik W, Alce TM
JournalJ Biol Chem
PubMed ID14570906
Despite the preferential localization of CD4 to lipid rafts, the significance and role of these microdomains in HIV-1 entry is still controversial. The possibility that CD4, when localized to non-raft domains, might be able to support virus entry cannot be excluded. Because disintegration of rafts by extraction of cellular cholesterol ... More
Growth repression in diethylstilbestrol/dimethylbenz[a]anthracene-induced rat mammary gland tumor using Hecate-CGbeta conjugate.
AuthorsZaleska M, Waclawik A, Bodek G, Zezula-Szpyra A, Li X, Janowski T, Hansel WH, Rahman NA, Ziecik AJ
JournalExp Biol Med (Maywood)
PubMed ID15044717
Recently, we have shown that Hecate-CGbeta conjugate, which is a fusion of the lytic peptide Hecate and a 15-amino acid fragment of the beta-chain of chorionic gonadotropin (CGbeta), selectively destroys mammary gland carcinoma cells that possess luteinizing hormone receptors (LHR) in vitro. We induced mammary gland tumors using combined prenatal ... More
The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) interacts with membrane type 1 matrix metalloproteinase and CD13/aminopeptidase N and modulates their endocytic pathways.
AuthorsMiki T, Takegami Y, Okawa K, Muraguchi T, Noda M, Takahashi C
JournalJ Biol Chem
PubMed ID17329256
The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) is anchored to the cell surface via glycosylphosphatidylinositol. This molecule antagonizes the function of membrane type 1 matrix metalloproteinase (MT1-MMP) to promote proMMP-2 maturation. Here, we attempt to clarify the mechanism underlying RECK functions. First, we found that RECK forms a complex ... More
Wnt2b controls retinal cell differentiation at the ciliary marginal zone.
AuthorsKubo F, Takeichi M, Nakagawa S
JournalDevelopment
PubMed ID12490564
The ciliary marginal zone of the vertebrate retina contains undifferentiated progenitor cells that continue to proliferate and add new neurons and glia peripherally during the embryonic stages - even after the formation of a functional retina. To understand the molecular mechanism that controls the prolonged progenitor cell proliferation in the ... More
Cell-type-dependent targeting of human immunodeficiency virus type 1 assembly to the plasma membrane and the multivesicular body.
AuthorsOno A, Freed EO
JournalJ Virol
PubMed ID14722309
The human immunodeficiency virus type 1 (HIV-1) assembly-and-release pathway begins with the targeting of the Gag precursor to the site of virus assembly. The molecular mechanism by which Gag is targeted to the appropriate subcellular location remains poorly understood. Based on the analysis of mutant Gag proteins, we and others ... More