Comparisons of CapG and gelsolin-null macrophages: demonstration of a unique role for CapG in receptor-mediated ruffling, phagocytosis, and vesicle rocketing.
AuthorsWitke W, Li W, Kwiatkowski DJ, Southwick FS
JournalJ Cell Biol
PubMed ID11514591
'Capping the barbed ends of actin filaments is a critical step for regulating actin-based motility in nonmuscle cells. The in vivo function of CapG, a calcium-sensitive barbed end capping protein and member of the gelsolin/villin family, has been assessed using a null Capg allele engineered into mice. Both CapG-null mice ... More
A fluorescence technique to distinguish attached from ingested erythrocytes and zymosan particles in phagocytosing macrophages.
AuthorsGreenberg S, el Khoury J, Kaplan E, Silverstein SC
JournalJ Immunol Methods
PubMed ID2040809
While investigating the role of [Ca2+]i in Fc receptor-mediated phagocytosis, we found that clamping the [Ca2+]i to low levels by a variety of methods often led to increased plasma membrane permeability, rendering the hypotonic lysis method for calculating the phagocytosis index unreliable. To overcome this difficulty we developed a method ... More