Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing
AuthorsSiala, M; Jaulhac, B; Gdoura, R; Sibilia, J; Fourati, H; Younes, M; Baklouti, S; Bargaoui, N; Sellami, S; Znazen, A; Barthel, C; Collin, E; Hammami, A; Sghir, A
JournalARTHRITIS RESEARCH & THERAPY
PubMed ID
Introduction Bacteria and/or their antigens have been implicated in the pathogenesis of reactive arthritis (ReA). Several studies have reported the presence of bacterial antigens and nucleic acids of bacteria other than those specified by diagnostic criteria for ReA in joint specimens from patients with ReA and various arthritides. The present ... More
Developmental Validation of Short Tandem Repeat Reagent Kit for Forensic DNA Profiling of Canine Biological Material
AuthorsDayton, M; Koskinen, MT; Tom, BK; Mattila, AM; Johnston, E; Halverson, J; Fantin, D; DeNise, S; Budowle, B; Smith, DG; Kanthaswamy, S
JournalCROATIAN MEDICAL JOURNAL
PubMed ID
Aim To develop a reagent kit that enables multiplex polymerase chain reaction (PCR) amplification of 18 short tandem repeats (STR) and the canine sex-determining Zinc Finger marker. Methods Validation studies to determine the robustness and reliability in forensic DNA typing of this multiplex assay included sensitivity testing, reproducibility studies, ... More
Phenotypic and genotypic diversity among strains of Aureobasidium pullulans in comparison with related species
AuthorsLoncaric, I; Oberlerchner, JT; Heissenberger, B; Moosbeckhofer, R
JournalAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
PubMed ID
Intra-specific diversity of 200 Aureobasidium pullulans strains isolated from different sources and their relatives Kabatiella lini CBS 125.21 T and Hormonema prunorum CBS 933.72 T were studied by assessment of macromorphological, and physiological tests, sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique (SDS-PAGE) of whole-cell proteins as well as enterobacterial repetitive intergenic ... More
Identification of common traits in improved xylose-growing Saccharomyces cerevisiae for inverse metabolic engineering
AuthorsBengtsson, O; Jeppsson, M; Sonderegger, M; Parachin, NS; Sauer, U; Hahn-Haegerdal, B; Gorwa-Grauslund, MF
JournalYEAST
PubMed ID
Four recombinant Saccharomyces cerevisiae strains with enhanced xylose growth (TMB3400, C1, C5 and BH42) were compared with two control strains (TMB3399, TMB3001) through genome-wide transcription analysis in order to identify novel targets for inverse metabolic engineering. A subset of 13 genes with changed expression levels in all improved strains was ... More
Primary extragastrointestinal stromal tumor of the transverse mesocolon without c-kit mutations but with PDGFRA mutations
AuthorsTerada, T
JournalMEDICAL ONCOLOGY
PubMed ID
The author reports a rare case of primary large cystic extragastrointestinal stromal tumor (eGIST) of the transverse mesocolon with genetic analyses of the c-kit and platelet-derived growth factor receptor-α (PDGFRA) genes. A 78-year-old man was found to have a large cystic tumor in the abdomen, and the tumor was resected. ... More
Cellulolytic streptomycetes from Sphagnum peat bogs and factors controlling their activity
AuthorsPankratov, TA; Dedysh, SN
JournalMICROBIOLOGY
PubMed ID
Two strains of Actinobacteria, ACTY and ACTR, were isolated from cellulolytic microbial communities obtained from an ombrotrophic Sphagnum peat bog. The strains were able to degrade cellulose, the main component of plant phytomass in this ecosystem. On the basis of their phenotypic and phylogenetic characteristics, the strains were identified as ... More
Development and genetic mapping of microsatellite markers from genome survey sequences in Brassica napus
Microsatellite or simple sequence repeat (SSR) markers are routinely used for tagging genes and assessing genetic diversity. In spite of their importance, there are limited numbers of SSR markers available for Brassica crops. A total of 627 new SSR markers (designated BnGMS) were developed based on publicly available genome survey ... More
Management units of the endangered herb Primula sieboldii based on microsatellite variation among and within populations throughout Japan
AuthorsHonjo, M; Kitamoto, N; Ueno, S; Tsumura, Y; Washitani, I; Ohsawa, R
JournalCONSERVATION GENETICS
PubMed ID
To promote programs for the conservation and restoration of the endangered species Primula sieboldii, we examined genetic variation at eight microsatellite loci among and within 32 remnant wild populations throughout Japan. Total allelic diversity within a population was higher in larger populations, but not so after rarefaction adjustment. The positive ... More
Nineteen novel microsatellite markers for the Olympia oyster, Ostrea conchaphila/lurida
AuthorsStick, DA; Langdon, CJ; Banks, MA; Camara, MD
JournalMolecular Ecology Resources
PubMed ID
Accurate evaluation of remnant Ostrea conchaphila/lurida population structure is critical for developing appropriate restoration efforts. Here we report 19 polymorphic microsatellites suitable for analyses of population differentiation, pedigree reconstruction and linkage map construction. We screened clones from four enriched genomic libraries, identified 73 microsatellite-containing sequences and designed polymerase chain reaction ... More
Multiple species of ectomycorrhizal fungi are frequently detected on individual oak root tips in a tropical cloud forest
The ecological importance of ectomycorrhizal (EM) fungi in tropical ecosystems is increasingly recognized, but few studies have used molecular methods to examine EM fungal communities in tropical forests. The diversity and composition of the EM community on Quercus crassifolia in a tropical montane cloud forest in southern Mexico were characterized ... More
Roles of naofen, a novel WD-repeat-2 protein, in the CCl(4)-treated livers - A possible relationship to cell proliferation
Naofen (GenBank accession no. EF613262), a newly found intracellular protein in the WD-repeat-2 protein family, has been cloned as an anti-verotoxin II antibody immunoreactive substance, and the nucleotide- and amino acid-sequences have been clarified. The present study was undertaken to evaluate the roles of naofen especially in carbon tetrachloride (CCl(4))-induced ... More
Detection of eight GMO maize events by qualitative, multiplex PCR and fluorescence capillary gel electrophoresis
AuthorsHeide, BR; Heir, E; Holck, A
JournalEUROPEAN FOOD RESEARCH AND TECHNOLOGY
PubMed ID
Specific legislation in the EU and several other countries requires that foods containing genetically modified organisms (GMOs) should be approved and labelled. This has necessitated the development of methods for detection of such materials. For screening purposes these methods should preferably enable detection of several different GMOs. Here we present ... More
Association of attentional network function with exon 5 variations of the CHRNA4 gene.
Mutational analyses in xenopus oocyte and mice models indicate that the positive effect of nicotine on attention may be modulated by genetic variations within exon 5 of the alpha4 subunit of the nicotinergic acetylcholine receptor gene CHRNA4. The potential relevance of exon 5 is further emphasized by two recent family-based ... More
The active conformation of avilamycin A is conferred by AviX12, a radical AdoMet enzyme.
AuthorsBoll R; Hofmann C; Heitmann B; Hauser G; Glaser S; Koslowski T; Friedrich T; Bechthold A
JournalThe Journal of Biological Chemistry
PubMed ID
The antibiotic avilamycin A is produced by Streptomyces viridochromogenes Tu57. Avilamycin belongs to the family of orthosomycins with a linear heptasaccharide chain linked to a terminal dichloroisoeverninic acid as aglycone. The gene cluster for avilamycin biosynthesis contains 54 open reading frames. Inactivation of one of these genes, namely aviX12, led ... More
Hypoxia up-regulates expression of Eph receptors and ephrins in mouse skin.
JournalThe Faseb Journal : Official Publication of the Federation of American Societies for Experimental Biology
PubMed ID
Eph receptor tyrosine kinases and their ligands (ephrins) are key players during the development of the embryonic vasculature; however, their role and regulation in adult angiogenesis remain to be defined. Both receptors and ligands have been shown to be up-regulated in a variety of tumors. To address the hypothesis that ... More
Long-range multilocus haplotype phasing of the MHC.
AuthorsGuo Z; Hood L; Malkki M; Petersdorf EW
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID
Haplotypes are a powerful tool for identifying the genetic basis of common complex diseases. Disease-association mapping requires molecular methods for haplotyping biallelic SNP variation and highly complex polymorphisms. We developed a method for phasing HLA-A, HLA-B, and HLA-DRB1 alleles on chromosome 6 in unrelated individuals. This method uses the highly ... More
The impact of CYP2C9 genetics and oral contraceptives on cytochrome P450 2C9 phenotype.
AuthorsSandberg M; Johansson I; Christensen M; Rane A; Eliasson E
JournalDrug Metabolism and Disposition: the Biological Fate of Chemicals
PubMed ID
CYP2C9-dependent drug metabolism is subject to large interindividual variation. To some extent, this is explained by genetic polymorphism with expression of enzyme variants that differ in catalytic activity. The aim of this study was to characterize the variation in CYP2C9 phenotype in relation to genotype, with further analysis of the ... More
Improvement in sensitivity of allele-specific PCR facilitates reliable noninvasive prenatal detection of cystic fibrosis.
AuthorsNasis O; Thompson S; Hong T; Sherwood M; Radcliffe S; Jackson L; Otevrel T
JournalClinical Chemistry
PubMed ID
BACKGROUND: Cell-free fetal DNA circulating in maternal blood has potential as a safer alternative to invasive methods of prenatal testing for paternally inherited genetic alterations, such as cystic fibrosis (CF) mutations. METHODS: We used allele-specific PCR to detect mutated CF D1152H DNA in the presence of an excess of the ... More
Role of hepatitis B virus genotypes Ba and C, core promoter and precore mutations on hepatocellular carcinoma: a case control study.
AuthorsYuen MF; Tanaka Y; Mizokami M; Yuen JC; Wong DK; Yuan HJ; Sum SM; Chan AO; Wong BC; Lai CL
JournalCarcinogenesis
PubMed ID
The role of hepatitis B virus (HBV) genotypes, core promoter (CP) and precore mutants on hepatocellular carcinoma (HCC) is still controversial. We aimed to determine their role on the development and clinical features of HCC. HBV genotypes and CP/precore mutations were determined in 90 HCC patients and 180 matched control ... More
Plakophilin 3 oncogene as prognostic marker and therapeutic target for lung cancer.
AuthorsFurukawa C; Daigo Y; Ishikawa N; Kato T; Ito T; Tsuchiya E; Sone S; Nakamura Y
JournalCancer Research
PubMed ID
We investigated gene expression profiles of non-small cell lung carcinomas (NSCLC) to screen candidate molecules that might be useful as diagnostic markers or for development of novel molecular-targeting therapies. Here we report evidence that a member of the armadillo protein family, plakophilin 3 (PKP3), is a potential molecular target for ... More
Unique catabolic pathway of glycosphingolipids in a hydrozoan, Hydra magnipapillata, involving endoglycoceramidase.
AuthorsHoribata Y; Sakaguchi K; Okino N; Iida H; Inagaki M; Fujisawa T; Hama Y; Ito M
JournalThe Journal of Biological Chemistry
PubMed ID
Endoglycoceramidase (EGCase; EC 3.2.1.123) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. We detected strong EGCase activity in animals belonging to Cnidaria, Mollusca, and Annelida and cloned the enzyme from a hydra, Hydra magnipapillata. The hydra EGCase, consisting of 517 amino acid ... More
Expression of claudin7 is tightly associated with epithelial structures in synovial sarcomas and regulated by an Ets family transcription factor, ELF3.
Synovial sarcoma, a soft tissue sarcoma that develops in adults, is pathologically subclassified into monophasic spindle synovial sarcoma and biphasic synovial sarcoma with epithelial components. The molecular mechanism building the epithelial components in biphasic synovial sarcoma is totally unknown. Here we investigated claudins, critical molecules in the tight junction, in ... More
Nonmammalian gonadotropin-releasing hormone molecules in the brain of promoter transgenic rats.
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID
Mammalian gonadotropin-releasing hormone (GnRH1) and nonmammalian immunoreactive GnRH subtypes were examined in transgenic rats carrying an enhanced GFP (EGFP) reporter gene driven by a rat GnRH1 promoter. Double-label immunocytochemistry was performed on EGFP(+)/GnRH1 brain sections by using antisera against GnRH1, GnRH2 (chicken II), GnRH3 (salmon), or seabream GnRH. EGFP(+)/GnRH1 neurons ... More
Quantitative analysis of diverse Lactobacillus species present in advanced dental caries.
AuthorsByun R; Nadkarni MA; Chhour KL; Martin FE; Jacques NA; Hunter N
JournalJournal of Clinical Microbiology
PubMed ID
Our previous analysis of 65 advanced dental caries lesions by traditional culture techniques indicated that lactobacilli were numerous in the advancing front of the progressive lesion. Production of organic acids by lactobacilli is considered to be important in causing decalcification of the dentinal matrix. The present study was undertaken to ... More
Alteration of pharmacokinetics of cyclophosphamide and suppression of the cytochrome p450 genes by ciprofloxacin.
AuthorsXie HJ; Broberg U; Griskevicius L; Lundgren S; Carlens S; Meurling L; Paul C; Rane A; Hassan M
JournalBone Marrow Transplantation
PubMed ID
Recently, it has been reported that prophylactic administration of ciprofloxacin during cyclophosphamide (CY) conditioning was a high-risk factor for relapse in patients undergoing allogeneic BMT. In the present study, we investigated the possible mechanisms of this interaction in male Sprague-Dawley rats. The kinetics of CY and its active 4-OH-CY metabolite ... More
Five molecular markers reveal extensive morphological homoplasy and reticulate evolution in the Malva alliance (Malvaceae)
The Malva alliance is a well-defined group with extensive morphological homoplasy. As a result, the relationships among the taxa as well as the evolution of morphological traits have remained elusive and the traditional classifications are highly artificial. Using five molecular markers (nuclear ITS, plastid matK plus trnK, ndhF, trnL-trnF, psbA-trnH), ... More
Effects of Mycoplasma pneumoniae infection on sphingolipid metabolism in human lung carcinoma A549 cells
'The role of sphingolipids in bacterial pathogenesis has been gradually recognized. In an effort to identify the possible involvement of sphingolipids during Mycoplasma pneumoniae (M. pneumoniae) infection, we first adopted a lipidomic approach to achieve the profiles of major sphingolipid species of M. pneumoniae as well as human lung carcinoma ... More
A functional role of gelatinase A in the development of nutritionally induced obesity in mice
AuthorsVan Hul, M; Lijnen, HR
Journal
PubMed ID
'Background: Development of adipose tissue is a complex process involving adipogenesis, angiogenesis and proteolytic remodeling of the extracellular matrix. The matrix metalloproteinase (MMP) system plays an important role in these processes. Objective: To establish a functional role of gelatinase A (MMP-2) in the development of adipose tissue. Methods: Mice with ... More
Association of RGS2 gene Polymorphisms with suicide and increased RGS2 immunoreactivity in the postmortem brain of suicide victims
AuthorsCui, H; Nishiguchi, N; Ivleva, E; Yanagi, M; Fukutake, M; Nushida, H; Ueno, Y; Kitamura, N; Maeda, K; Shirakawa, O
Journal
PubMed ID
'Regulators of G-protein signaling are a family of proteins that negatively regulate the intracellular signaling of G protein-coupled receptors, such as the serotonin receptor. Recent studies have suggested that one of these proteins, the regulator of G-protein signaling 2 (RGS2), plays an important part in anxiety and/or aggressive behavior. To ... More
Country-wide assessment of the genetic polymorphism in Plasmodium falciparum and Plasmodium vivax antigens detected with rapid diagnostic tests for malaria
AuthorsMariette, N; Barnadas, C; Bouchier, C; Tichit, M; Menard, D
Journal
PubMed ID
'Background: Rapid diagnostic tests (RDTs) are becoming increasingly indispensable in malaria management, as a means of increasing the accuracy of diagnosis. The WHO has issued recommendations, but the selection of the most suitable RDT remains difficult for users in endemic countries. The genetic variability of the antigens detected with RDTs ... More
Polymorphisms in steroid hormone biosynthesis genes and risk of breast cancer and fibrocystic breast conditions in Chinese women
'Common variants in genes encoding for key enzymes involved in steroidogenesis may alter sex steroid hormone levels, thereby influencing susceptibility to breast carcinoma and related conditions. In a case-control study of Chinese women, we examined genotypes of the CYP11A1 pentanucleotide [(TAAAA)(n)] repeat (D15S520), CYP17A1 rs743572, and HSD17B1 rs605059 polymorphisms in ... More
Selective Contribution of Interleukin-6 and Leptin to Brain Inflammatory Signals Induced by Systemic LPS Injection in Mice
AuthorsRummel, C; Inoue, W; Sachot, C; Poole, S; Huebschle, T; Luheshi, GN
Journal
PubMed ID
'This study aimed to address the relative contributions of the proinflammatory cytokine interleukin-6 (IL-6) and the cytokine-like hormone leptin to the genomic activation of brain cells during lipopolysaccharide (LPS)-induced systemic inflammation. Wildtype and IL-6KO mice were injected with LPS (50 μg/kg, intraperitoneally) and the brains analyzed by immunohistochemistry and reverse-transcriptase ... More
Genetic variation in the transforming growth factor-β1 gene is associated with susceptibility to IgA nephropathy
AuthorsMai, TV; Lundberg, S; Gunnarsson, I; Wramner, L; Seddighzadeh, M; Hahn-Zoric, M; Fernstroem, A; Hanson, LA; Lieu, TD; Jacobson, SH; Padyukov, L
Journal
PubMed ID
'Background. There is growing evidence of genetic risk for susceptibility to IgA nephropathy. Among several candidate genes related to immunological regulation in renal tissue, TGFB1 is known to be a contributor to proliferation and the development of fibrosis. Methods. We analysed several SNPs in a region of this gene using ... More
Evidence for both lytic replication and tightly regulated human herpesvirus 8 latency in circulating mononuclear cells, with virus loads frequently below common thresholds of detection.
AuthorsMartro E; Cannon MJ; Dollard SC; Spira TJ; Laney AS; Ou CY; Pellett PE
Journal
PubMed ID
'To address whether human herpesvirus 8 (HHV-8) DNA in peripheral blood mononuclear cells (PBMCs) might be the product of latent or lytic infection and to shed light on sporadic detection of HHV-8 DNA in individuals seropositive for the virus, we studied the frequency of infected cells, total virus load, and ... More
Expression of functional nicotinic acetylcholine receptors in rat urinary bladder epithelial cells.
AuthorsBeckel JM; Kanai A; Lee SJ; de Groat WC; Birder LA
Journal
PubMed ID
'Although nicotinic acetylcholine receptors in both the central and peripheral nervous systems play a prominent role in the control of urinary bladder function, little is known regarding expression or function of nicotinic receptors in the bladder epithelium, or urothelium. Nicotinic receptors have been described in epithelial cells lining the upper ... More
Autosomal recessive retinitis pigmentosa is associated with mutations in RP1 in three consanguineous Pakistani families.
'PURPOSE: To localize and identify the gene and mutations causing autosomal recessive retinitis pigmentosa in three consanguineous Pakistani families. METHODS: Blood samples were collected and DNA was extracted. A genome-wide scan was performed by using 382 polymorphic microsatellite markers on genomic DNA from affected and unaffected family members, and lod ... More
Discrimination between the main activating and inhibitory killer cell immunoglobulin-like receptor positive natural killer cell subsets using newly characterized monoclonal antibodies
AuthorsDavid, G; Morvan, M; Gagne, K; Kerdudou, N; Willem, C; Devys, A; Bonneville, M; Follea, G; Bignon, JD; Retiere, C
Journal
PubMed ID
'Natural killer (NK) cells are key components of the innate anti-viral and anti-tumour immune responses. NK cell function is regulated by the interaction of killer cell immunoglobulin-like receptors (KIR) with human leucocyte antigen (HLA) class I molecules. In this study, we report on the generation of KIR-specific antibodies allowing for ... More
RASSF1A and NORE1A methylation and BRAFV600E mutations in thyroid tumors.
'We analyzed RASSF1A and NORE1A methylation and BRAF mutation in 89 thyroid tumors, 42 non-neoplastic thyroid tissues and three thyroid tumor cell lines using polymerase chain reaction (PCR), methylation-specific PCR, Western blotting and DNA sequencing in order to study thyroid tumor pathogenesis and progression. RASSF1A promoter methylation was present in ... More
Simple polymerase chain reaction for the detection of mutations and deletions in the epidermal growth factor receptor gene: Applications of this method for the diagnosis of non-small-cell lung cancer
AuthorsUhara, M; Matsuda, K; Taira, C; Higuchi, Y; Okumura, N; Yamauchi, K
Journal
PubMed ID
'Background: Somatic mutations in the epidermal growth factor receptor (EGFR) gene are associated with the responses to the tyrosine kinase inhibitors gefitinib and erlotinib in patients with non-small-cell lung cancer (NSCLC). Although various methods for detecting EGFR gene mutations have been developed, they have several disadvantages. We attempted to establish ... More
Down-regulation of pregnane X receptor contributes to cell growth inhibition and apoptosis by anticancer agents in endometrial cancer cells.
AuthorsMasuyama H; Nakatsukasa H; Takamoto N; Hiramatsu Y
Journal
PubMed ID
'Recent studies have revealed that pregnane X receptor (PXR) can function as a master regulator to control the expression of drug-metabolizing enzymes, cytochrome P450 3A (CYP3A) family, and members of the drug transporter family, including multiple drug resistance 1 (MDR1). We demonstrated previously that steroid/xenobiotic metabolism by tumor tissue through ... More
Differential telomere processing by Borrelia telomere resolvases in vitro but not in vivo.
AuthorsTourand Y; Bankhead T; Wilson SL; Putteet-Driver AD; Barbour AG; Byram R; Rosa PA; Chaconas G
Journal
PubMed ID
'Causative agents of Lyme disease and relapsing fever, including Borrelia burgdorferi and Borrelia hermsii, respectively, are unusual among bacteria in that they possess a segmented genome with linear DNA molecules terminated by hairpin ends, known as telomeres. During replication, these telomeres are processed by the essential telomere resolvase, ResT, in ... More
Quadruplex genotyping of F5, F2, and MTHFR variants in a single closed tube by high-resolution amplicon melting.
'BACKGROUND: Multiplexed amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, asymmetric PCR, or allele-specific PCR; however, correct differentiation of homozygous mutant and wild-type samples by melting temperature (T(m)) analysis requires high-resolution melting analysis and controlled reaction conditions. METHODS: We designed 4 amplicons bracketing ... More
Proteolytic excision of a repressive loop domain in tartrate-resistant acid phosphatase by cathepsin K in osteoclasts.
AuthorsLjusberg J; Wang Y; Lang P; Norgard M; Dodds R; Hultenby K; Ek-Rylander B; Andersson G
Journal
PubMed ID
'Tartrate-resistant acid phosphatase (TRAP) is a metallophosphoesterase participating in osteoclast-mediated bone turnover. Activation of TRAP is associated with the redox state of the di-iron metal center as well as with limited proteolytic cleavage in an exposed loop domain. The cysteine proteinases cathepsin B, L, K, and S as well as ... More
Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats
AuthorsJumabay, M; Matsumoto, T; Yokoyama, S; Kano, K; Kusumi, Y; Masuko, T; Mitsumata, M; Saito, S; Hirayama, A; Mugishima, H; Fukuda, N
Journal
PubMed ID
'Adipose tissue-derived stem cells have been demonstrated to differentiate into cardiomyocytes and vascular endothelial cells. Here we investigate whether mature adipocyte-derived dedifferentiated fat (DFAT) cells can differentiate to cardiomyocytes in vitro and in vivo by establishing DFAT cell lines via ceiling culture of mature adipocytes. DFAT cells were obtained by ... More
Genetic analysis of the GRM1 gene in human melanoma susceptibility.
'Data obtained from a mouse model indicated that the ectopic expression of the Grm1 gene is sufficient for transforming melanocytes and causing malignant melanoma in vivo. In addition, it has also been documented that the GRM1 gene is aberrantly expressed in human melanomas. Here we have performed a genetic association ... More
Two mutations in the HSN2 gene explain the high prevalence of HSAN2 in French Canadians.
AuthorsRoddier K; Thomas T; Marleau G; Gagnon AM; Dicaire MJ; St-Denis A; Gosselin I; Sarrazin AM; Larbrisseau A; Lambert M; Vanasse M; Gaudet D; Rouleau GA; Brais B
Journal
PubMed ID
'BACKGROUND: Hereditary sensory and autonomic neuropathy type 2 (HSAN2; MIM 201300) is a rare recessive neuropathy typically diagnosed in the first decade. The 1973 study of a French Canadian family led to the definition of HSAN2. OBJECTIVES: To demonstrate that the apparent higher prevalence of HSAN2 in Quebec is due ... More
Competitive facilitation of drug-resistant Plasmodium falciparum malaria parasites in pregnant women who receive preventive treatment
AuthorsHarrington, WE; Mutabingwa, TK; Muehlenbachs, A; Sorensen, B; Bolla, MC; Fried, M; Duffy, PE
Journal
PubMed ID
'Intermittent preventive treatment in pregnancy (IPTp) is used to prevent Plasmodium falciparum malaria. However, parasites resistant to the IPTp drug sulfadoxine-pyrimethamine (SP) have emerged worldwide, and infections with mixed resistant and susceptible parasites are exacerbated by pyrimethamine in mice. In a prospective delivery cohort in Muheza, Tanzania, we examined the ... More
A region of barley chromosome 6H harbors multiple major genes associated with net type net blotch resistance
'Net type net blotch (NTNB), caused by Pyrenophora teres f. teres Drechs., is prevalent in barley growing regions worldwide. A population of 118 doubled haploid (DH) lines developed from a cross between barley cultivars ''Rika'' and ''Kombar'' were used to evaluate resistance to NTNB due to their differential reaction to ... More
Relation of Genetic Polymorphisms in the Cytochrome P450 Gene With Clopidogrel Resistance After Drug-Eluting Stent Implantation in Koreans
AuthorsLee, JM; Park, S; Shin, DJ; Choi, D; Shim, CY; Ko, YG; Kim, JS; Shin, ES; Chang, CW; Lee, JE; Jang, Y
Journal
PubMed ID
'Clopidogrel is a prodrug that has to be converted to an active metabolite by hepatic cytochrome P450 (CYP) isoenzymes to inhibit platelet aggregation. Individual variability of platelet inhibition by clopidogrel suggests a possibility for genetic factors having a significant influence on clopidogrel responsiveness. In this study, we sought to determine ... More
Identification and characterization of epp, the secreted processing protease for the Vibrio anguillarum EmpA metalloprotease
AuthorsVarina, M; Denkin, SM; Staroscik, AM; Nelson, DR
Journal
PubMed ID
'The zinc metalloprotease EmpA is a virulence factor for the fish pathogen Vibrio anguillarum. Previous studies demonstrated that EmpA is secreted as a 46-kDa proenzyme that is activated extracellularly by the removal of an ∼10-kDa propeptide. We hypothesized that a specific protease is responsible for processing secreted pro-EmpA into mature ... More
Gene flow and hybridisation in a mixed oak forest (Quercus pyrenaica Willd. and Quercus petraea (Matts.) Liebl.) in central Spain.
AuthorsValbuena-Carabana M; Gonzalez-Martinez SC; Sork VL; Collada C; Soto A; Goicoechea PG; Gil L
Journal
PubMed ID
'Oaks are long-standing models for the study of gene flow and hybridisation. Temperate (Quercus petraea) and sub-Mediterranean (Quercus pyrenaica) oaks coexist in central Spain, showing remarkable differences in population size and structure. Q. petraea has a scattered distribution in central Spain, where it is at one of the southernmost limits ... More
Lack of correlation between phenotype and genotype in untreated 21-hydroxylase-deficient Indonesian patients
'Background Mutations in CYP21A2 lead to deficiency of 21-hydroxylase and can have either severe or moderate effects on phenotype, which can be prevented by early treatment. We studied long-term effects of this deficiency on phenotype in patients who had not been treated for prolonged periods and correlated these phenotypes ... More
Expression, Localization, and Binding Activity of the Ezrin/Radixin/Moesin Proteins in the Mouse Testis
AuthorsWakayama, T; Nakata, H; Kurobo, M; Sai, Y; Iseki, S
Journal
PubMed ID
'The ezrin, radixin, and moesin (ERM) proteins represent a family of adaptor proteins linking transmembrane proteins to the cytoskeleton. The seminiferous epithelium undergoes extensive changes in cellular composition, location, and shape, implicating roles of the membrane-cytoskeleton interaction. It remains unknown, however, whether the ERM proteins are expressed and play significant ... More
Allele *1 of HS1.2 Enhancer Associates with Selective IgA Deficiency and IgM Concentration
AuthorsGiambra, V; Cianci, R; Lolli, S; Mattioli, C; Tampella, G; Cattalini, M; Kilic, SS; Pandolfi, F; Plebani, A; Frezza, D
Journal
PubMed ID
'Selective IgA deficiency (IGAD) is the most common primary immunodeficiency, yet its pathogenesis is elusive. The IG (heavy) H chain human 3'' Regulatory Region harbors three enhancers and has an important role In Ig synthesis. HS1.2 is the only polymorphic enhancer of the 3''RRs. We therefore evaluated HS1.2 allelic frequencies ... More
Synergistic effect of galantamine on nicotine-induced neuroprotection in hemiparkinsonian rat model
'Recent studies have reported that smokers tend to be less susceptible to Parkinson''s disease (PD) and the stimulation of nicotinic acetylcholine receptor (nAChR) is considered to confer a neuroprotective effect. Galantamine is an acetylcholinesterase inhibitor and an allosteric potentiating ligand for nAChRs. However, the effects of galantamine and nicotine on ... More
Detecting pathway-based gene-gene and gene-environment interactions in pancreatic cancer
'Data mining and data reduction methods to detect interactions in epidemiologic data are being developed and tested. In these analyses, multifactor dimensionality reduction, focused interaction testing framework, and traditional logistic regression models were used to identify potential interactions with up to three factors. These techniques were used in a population-based ... More
A Bayesian approach for discriminating among alternative inheritance hypotheses in plant polyploids: the allotetraploid origin of genus Borderea (Dioscoreaceae).
AuthorsCatalan P; Segarra-Moragues JG; Palop-Esteban M; Moreno C; Gonzalez-Candelas F
Journal
PubMed ID
'Polyploidy is a common phenomenon occurring in a vast number of land plants. Investigations of patterns of inheritance and the origins of plants (i.e., autopolyploidy vs. allopolyploidy) usually involve cytogenetic and molecular studies of chromosome pairing, chromosome mapping, and marker segregation analysis through experimental crosses and progeny tests. Such studies ... More
Thiabendazole resistance and mutations in the β-tubulin gene of Penicillium expansum strains isolated from apples and pears with blue mold decay
'Penicillium expansum is the causal agent of blue mold rot, a postharvest decay of stored fruits. This fungus also produces the mycotoxins patulin and citrinin. Control of P. expansum still relies mainly on the use of fungicides such as thiabendazole. Since its introduction, resistant strains have been reported. The aim ... More
Oligonucleotide properties determination and primer designing: a critical examination of predictions.
AuthorsChavali S; Mahajan A; Tabassum R; Maiti S; Bharadwaj D
Journal
PubMed ID
'MOTIVATION: Precise prediction of melting temperature (T(m)), secondary structures and design of oligonucleotides determine the efficiency and success of experimentation in molecular biology. Availability of a plethora of software and the users unawareness about their limitations compromises the accuracy and reliability of the predictions. RESULTS: Comparative analysis of 56 modules ... More
PCR testing for Treponema pallidum in paraffin-embedded skin biopsy specimens: test design and impact on the diagnosis of syphilis.
AuthorsBehrhof W; Springer E; Brauninger W; Kirkpatrick CJ; Weber A
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PubMed ID
'BACKGROUND: Syphilis, a chronic infection caused by Treponema pallidum, is a disease which is increasing in incidence, and thus more and more becoming a differential diagnosis in routine pathology. AIMS: To develop a PCR-based molecular assay for the detection of T pallidum in formalin-fixed, paraffin-embedded tissues, and evaluate its diagnostic ... More
Bone augmentation by bone marrow mesenchymal stem cells cultured in three-dimensional biodegradable polymer scaffolds
AuthorsTanaka, T; Hirose, M; Kotobuki, N; Tadokoro, M; Ohgushi, H; Fukuchi, T; Sato, J; Set, K
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'Poly-lactic-glycolic acid (PLGA) is a biocompatible as well as biodegradable polymer and used in various medical applications. In this study, we evaluated efficiency of the specially designed three-dimensional porous PLGA as a scaffold for bone augmentation. First, cell attachment/proliferation, differentiation, and mineralization of Fisher 344 rat marrow mesenchymal stem cells ... More
TNF-α and IL-10 gene polymorphisms versus cardioimmunological responses in sudden infant death
'We hypothesized that genetic variations of cytokines could contribute to the risk of developing a fatal immunological reaction in the heart of infants. Thus, tumor necrosis factor (TNF)-α and interleukin (IL)-10 gene polymorphisms versus induction of cardioimmunologxical responses in victims of sudden infant death syndrome (SIDS) were explored. We genotyped ... More
Differential radiation sensitivity to morphological, functional and molecular changes of human thyroid tissues and bone marrow cells maintained in SCID mice
'Morphology and function of human organs and tissues are well maintained in the improved SCID (severe combined immunodeficient) mice for a long period (∼3 years). To study the radiation-induced damage on human thyroid gland, human thyroid tissues transplanted to SCID mice were consecutively exposed to Xrays or (137)Cs γ-rays at ... More
Evaluation of eotaxin-1, -2, and -3 protein production and messenger RNA expression in patients with vernal keratoconjunctivitis
AuthorsShoji, J; Inada, N; Sawa, M
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'To evaluate the in vivo expression of members of the eotaxin subfamily, eotaxin-1, -2, and -3, at the ocular surface, we analyzed the messenger RNA (mRNA) expression of the eotaxin subfamily in conjunctival epithelium and the protein expression of the eotaxin subfamily in tears of patients with vernal keratoconjunctivitis (VKC) ... More
Isolation and characterization of alginate-degrading bacteria for disposal of seaweed wastes
AuthorsTang, JC; Taniguchi, H; Chu, H; Zhou, Q; Nagata, S
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'Isolation of novel alginate degrading bacteria for the disposal of seaweed waste in composting process. Decomposition of alginate polymers was checked by the 3,5-dinitrosalicylic acid (DNS) method for reducing sugar, and absorbance at 235 nm for unsaturated sugar. A bacterium A7 was isolated from wakame compost and confirmed to belong ... More
Specific Detection of Viable Legionella Cells by Combined Use of Photoactivated Ethidium Monoazide and PCR/Real-Time PCR
'Legionella organisms are prevalent in manmade water systems and cause legionellosis in humans. A rapid detection method for viable Legionella cells combining ethidium monoazide (EMA) and PCR/real-time PCR was assessed. EMA could specifically intercalate and cleave the genomic DNA of heat- and chlorine-treated dead Legionella cells. The EMA-PCR assay clearly ... More
Vancomycin heteroresistance and biofilm formation in Staphylococcus epidermidis from food
AuthorsGazzola, S; Cocconcelli, PS
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'Staphylococcus epidermidis CNBL 7032 is a heteroresistant strain, with subpopulations resistant to vancomycin concentrations up to 32 mg 1(-1), which was isolated from cured pork meat. The mechanisms of glycopeptide resistance in this strain were investigated in this study. S. epidermidis CNBL 7032 does not harbour enterococcal transmissible vancomycin-resistance genes. ... More
Association of TBX21 polymorphisms in a Korean population with rheumatoid arthritis
AuthorsChae, SC; Shim, SC; Chung, HT
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'TBX21 (T-bet) is a member of the T-box family of transcriptional factors that contain a conserved DNA binding domain. TBX21 is a critical regulator of the commitment to the Th1 lineage and IFN-γ production. Th1 and Th2 cells cross-regulate the differentiation of each other, and in this way TBX21 could ... More
Nystatin interferes with the effects of N-methyl-N′-nitro-N-nitrosoguanidine on sphingolipid metabolism in human FL cells
'Previously we have shown that an alkylating agent N-methyl-N''-nitro-N- nitrosoguanidine (MNNG) can induce receptor clustering and the activation of a downstream signal molecule NF-κB, and that the receptor clustering is associated with changes in sphingolipids metabolism. On the other hand, the polyene antibiotic nystatin can block MNNG-induced receptor clustering. In ... More
The existence of a local 5-hydroxytryptaminergic system in peripheral arteries
'Background and purpose: 5-HT is a vasoconstrictor exhibiting enhanced effects in systemic arteries from subjects with cardiovascular disease. The effect of endogenous 5-HT on arteries is controversial, because the concentration of free circulating 5-HT is low and a 5-hydroxytryptaminergic system has not been identified in peripheral arteries. We hypothesized that ... More
Coordinated changes in xenobiotic metabolizing enzyme gene expression in aging male rats
'In order to gain insight into the effects of aging on susceptibility to environmental toxins, we characterized the expression of xenobiotic metabolizing enzymes (XMEs) from the livers of male F344 and Brown Norway (BN) rats across the adult lifespan. Using full-genome Affymetrix arrays, principal component analysis showed a clear age-dependent ... More
A chloroplast genealogy of hordeum (poaceae): Long-term persisting haplotypes, incomplete lineage sorting, regional extinction, and the consequences for phylogenetic inference.
AuthorsJakob SS; Blattner FR
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'To analyze reasons for inconclusive results of earlier chloroplast phylogenies in the grass genus Hordeum, we established a genealogy of chloroplast haplotypes by sequencing the trnL-trnF region in 875 individuals, covering all 31 species of the genus. Although the outcomes of phenetic and parsimony analyses of 88 haplotypes were ambiguous, ... More
Detection of the sul1, sul2, and sul3 genes in sulfonamide-resistant bacteria from wastewater and shrimp ponds of north Vietnam
AuthorsHoa, PTP; Nonaka, L; Viet, PH; Suzuki, S
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'To assess the presence and distribution of the sul genes (sul1, sul2, and sul3) and plasmids in human-mediated environments of north Vietnam, we examined a total of 127 sulfonamide-resistant (SR) bacterial isolates from four shrimp ponds (HNAQs), a city canal (HNCs) and three fish ponds that received wastewater directly from ... More
Functional characterization of two isoforms of the P2Y-like receptor GPR17: [(35)S]GTPγS binding and electrophysiological studies in 1321N1 cells
'Pugliese AM, Trincavelli ML, Lecca D, Coppi E, Fumagalli M, Ferrario S, Failli P, Daniele S, Martini C, Pedata F, Abbracchio MP. Functional characterization of two isoforms of the P2Y-like receptor GPR17: [(35)S]GTPγS binding and electrophysiological studies in 1321N1 cells. Am J Physiol Cell Physiol 297: C1028-C1040, 2009. First published ... More
Induction of proinflammatory cytokines by long-chain saturated fatty acids in human macrophages
AuthorsHaaversen, L; Danielsson, KN; Fogelstrand, L; Wiklund, O
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'Increased circulating free fatty acids in subjects with type 2 diabetes may contribute to activation of macrophages, and thus the development of atherosclerosis. In this study, we investigated the effect of the saturated fatty acids (SFA) palmitate, stearate, myristate and laurate, and the unsaturated fatty acid linoleate, on the production ... More
Spectrum of factor VIII mutations in Arab patients with severe haemophilia A
AuthorsAbu-Amero, KK; Hellani, A; Al-Mahed, M; Al-Sheikh, I
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'Haemophilia A is an X-linked recessive bleeding disorder caused by mutations in the factor VIII (FVIII) gene. The mutation spectrum is known in various populations, but not in Arabs. We selected 20 unrelated Arab patients with severe haemophilia A. Those patients underwent detailed clinical examination and their plasma FVIII:C activity ... More
Phylogenetic relationships among the species of Elymus sensu lato in Triticeae (Poaceae) based on nuclear rDNA ITS sequences
'The genus Elymus L. sensu lato includes Roegneria, Elymus, Hystrix, Sitanion and Kengyilia, and they are very important group in the tribe Triticeae. However, the phylogenetic relationships and taxonomic status of them are still in dispute. The ITS sequences were obtained and analyzed for their phylogenetic relationships by using Maximum ... More
Chronic colonization with Pandoraea apista in cystic fibrosis patients determined by repetitive-element-sequence PCR.
AuthorsAtkinson RM; Lipuma JJ; Rosenbluth DB; Dunne WM Jr
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'Pandoraea apista is recovered with increasing frequency from the lungs of patients with cystic fibrosis (CF) and may represent an emerging pathogen (I. M. Jorgensen et al., Pediatr. Pulmonol. 36:439-446, 2003). We identified two CF patients from our hospital whose sputum specimens were culture positive for P. apista over the ... More
Impact of EGFR immunoexpression on STAT3 activation and association with proinflammatory/regulatory cytokine pattern in laryngeal squamous cell carcinoma
AuthorsStarska, K; Brys, M; Forma, E; Glowacka, E; Lewy-Trenda, I; Stasikowska, O; Krajewska, WM; Lukomski, M
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'Stimulation of epidermal growth factor receptor (EGFR) results in the activation of signal transducer and activator of transcription-3 (STAT3), a transcriptional factor associated with carcinogenesis. Proinflammatory cytokines are capable of activating a tumor cell death program by reducing EGFR tyrosine phosphorylation. This study aimed to identify EGFR expression in laryngeal ... More
Positive Selection Results in Frequent Reversible Amino Acid Replacements in the G Protein Gene of Human Respiratory Syncytial Virus
'Human respiratory syncytial virus (HRSV) is the major cause of lower respiratory tract infections in children under 5 years of age and the elderly, causing annual disease outbreaks during the fall and winter. Multiple lineages of the HRSVA and HRSVB serotypes co-circulate within a single outbreak and display a strongly ... More
Occurrence of two genotypes of tetracycline (TC) resistance gene tet(M) in the TC-resistant bacteria in marine sediments of Japan
AuthorsRahman, MH; Nonaka, L; Tago, R; Suzuki, S
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'The tetracycline (TC) resistance gene team) was monitored in bacteria isolated from Japanese coastal and off-shore marine sediments. The high rate of occurrence of TC resistant (TC(r)) bacteria (120 μg mL(-1) TC) was observed at frequency ranges between 0.0-0.08% in Tokyo Bay, 1.67-1.82% in Sagami Bay and 0.0-4.35% in the ... More
FOXP3 Inhibits Activation-Induced NFAT2 Expression in T Cells Thereby Limiting Effector Cytokine Expression
'The forkhead DNA-binding protein FOXP3 is critical for the development and suppressive function of CD4(+)CD25(+) regulatory T cells (T(REG)), which play a key role in maintaining self-tolerance. Functionally, FOXP3 is capable of repressing transcription of cytokine genes regulated by NEAT. Various mechanisms have been proposed by which FOXP3 mediates these ... More
Selection of bacterial populations in the mycosphere of Laccaria proxima: is type III secretion involved?
AuthorsWarmink, JA; van Elsas, JD
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'The bacterial communities in the Laccaria proxima mycosphere (soil from beneath fruiting bodies) and the corresponding bulk soil were compared by cultivation-dependent and cultivation-independent methods. To assess the distribution of type III secretion systems (TTSS), a PCR-based system for the broad detection of a highly conserved gene involved in TTSS, ... More
Genomic identification in the historical case of the Nicholas II royal family
'Accurate unambiguous identification of ancient or historical specimens can potentially be achieved by DNA analysis. The controversy surrounding the fate of the last Russian Emperor, Nicholas II, and his family has persisted, in part, because the bodies of 2 children, Prince Alexei and 1 of his sisters, have not been ... More
'The genus Listeria comprises six closely related bacterial species that share considerable morphological, ecological, biochemical and molecular similarities. In this study, through examination of a large collection (n = 84) of Listeria monocytogenes strains, we identified four serovar 4c strains (i.e., RM3894, RM3899, RM3901 and RM3905) that cross-reacted with Listeria ... More
The C609T variant of NQ01 is associated with carotid artery plaques in patients with type 2 diabetes
AuthorsHan, SJ; Kang, ES; Kim, HJ; Kim, SH; Chun, SW; Ahn, CW; Cha, BS; Nam, M; Lee, HC
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'Objective: Atherosclerosis in patients with type 2 diabetes has been linked to oxidative stress. NADP[1]:quinone oxidoreductase 1 (NQO1) plays a key role in cellular antioxidant defense. Recent reports suggest that highly expressed and inducible enclogenous NQO1 from cardiovascular cells may act as a potential superoxide scavenger. We examined the relationship ... More
No genetic association of the human prolyl endopeptidase gene in the Dutch celiac disease population.
AuthorsDiosdado B; Stepniak DT; Monsuur AJ; Franke L; Wapenaar MC; Mearin ML; Koning F; Wijmenga C
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'Celiac disease (CD) is a complex genetic disorder of the small intestine. The DQ2/DQ8 human leucocyte antigen (HLA) genes explain approximately 40% of the genetic component of the disease, but the remaining non-HLA genes have not yet been identified. The key environmental factor known to be involved in the disease ... More
Molecular evidence for fragmentation among populations of Taxus wallichiana var. mairei, a highly endangered conifer in China
AuthorsZhang, XM; Gao, LM; Moeller, M; Li, DZ
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'Taxus wallichiana var. mairei (Lemee & Leveille) L. K. Fu & Nan Li is a highly endangered conifer in China. It is dioecious and wind pollinated, with seed dispersal by animals. Extant natural populations on mainland China and Taiwan are highly scattered, largely as a result of habitat loss and ... More
Invasion of Lotus japonicus root hairless 1 by Mesorhizobium loti involves the nodulation factor-dependent induction of root hairs.
AuthorsKaras B; Murray J; Gorzelak M; Smith A; Sato S; Tabata S; Szczyglowski K
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'In many legumes, including Lotus japonicus and Medicago truncatula, susceptible root hairs are the primary sites for the initial signal perception and physical contact between the host plant and the compatible nitrogen-fixing bacteria that leads to the initiation of root invasion and nodule organogenesis. However, diverse mechanisms of nodulation have ... More
Detection and quantification of genetically modified organisms using very short, locked nucleic acid TaqMan probes
AuthorsSalvi, S; D'Orso, F; Morelli, G
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'Many countries have introduced mandatory labeling requirements on foods derived from genetically modified organisms (GMOs). Real-time quantitative polymerase chain reaction (PCR) based upon the TaqMan probe chemistry has become the method mostly used to support these regulations; moreover, event-specific PCR is the preferred method in GMO detection because of its ... More
Phenotypic and Genotypic Characteristics of Mastocytosis According to the Age of Onset
'Adult''s mastocytosis is usually associated with persistent systemic involvement and c-kit 816 mutation, while pediatrics disease is mostly limited to the skin and often resolves spontaneously. We prospectively included 142 adult patients with histologically proven mastocytosis. We compared phenotypic and genotypic features of adults patients whose disease started during childhood ... More
Glutathione S-transferase M1 and P1 polymorphisms and risk of breast cancer and fibrocystic breast conditions in Chinese women
'Enzymes encoded by the glutathione S-tranferase mu 1 (GSTM1) and pi 1 (GSTP1) genes, which are expressed in breast tissue, catalyze the detoxification of endogenous and exogenous electrophiles. Reduced enzyme activity, due to carriage of the GSTM1 deletion or the GSTP1 Ile105Val Val allele, may therefore affect susceptibility to breast ... More
Cancer-associated splicing variants of the CDCA1 and MSMB genes expressed in cancer cell lines and surgically resected gastric cancer tissues
'Background. Alternative splicing is a molecular mechanism by which different combinations of exons can be alternatively spliced to produce different mRNA isoforms. Recently, several databases have been published to predict the alto-native splicing of mRNA; cancer-specific alternative splicing has also been predicted with these databases. Those variants may be potentially ... More
Genetic variants in COMT and neurocognitive impairment in families of patients with schizophrenia
'This study examined the relations of genetic variants in catechol-O-methyltransferase (COMT) gene, including rs737865 in intron 1, rs4680 in exon 4 (Val158Met) and downstream rs165599, to schizophrenia and its related neurocognitive functions in families of patients with schizophrenia. Totally, 680 individuals from 166 simplex (166 affected members and 354 nonpsychotic ... More
Identification and cloning of the α2-macroglobulin of giant freshwater prawn Macrobrachium rosenbergii and its expression in relation with the molt stage and bacteria injection
AuthorsHo, PY; Cheng, CH; Cheng, WT
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'The full-length complementary (c)DNA of the α2-macroglobulin (α2M) gene was cloned from haemocytes of the giant freshwater prawn Macrobrachium rosenbergii by reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) methods. The 4875-bp cDNA contains an open reading frame (ORF) of 4419 bp, a 95-bp 5''-untranslated region ... More
Development of a multilocus variable-number of tandem repeat typing method for Listeria monocytogenes serotype 4b strains
AuthorsMiya, S; Kimura, B; Sato, M; Takahashi, H; Ishikawa, T; Suda, T; Takakura, C; Fujii, T; Wiedmann, M
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'Listeria monocytogenes serotype 4b strains have been identified as the Causative agent in many human listeriosis epidemics as well as in a considerable number of sporadic cases. Due to the genetic homogeneity of serotype 4b isolates, development of rapid subtyping methods with high discriminatory power for serotype 4b isolates is ... More
Molecular epidemiology of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from global clinical trials
AuthorsGoering, RV; Shawar, RM; Scangarella, NE; O'Hara, FP; Amrine-Madsen, H; West, JM; Dalessandro, M; Becker, JA; Walsh, SL; Miller, LA; van Horn, SF; Thomas, ES; Twynholm, ME
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'Determining the genetic characteristics of Staphylococcus aureus is important for better understanding of the global and dynamic epidemiology of this organism as we witness the emergence and spread of virulent and antibiotic-resistant clones. We genotyped 292 S. aureus isolates (105 methicillin resistant and 187 methicillin susceptible) using a combination of ... More
Preclinical Characterization of PF-00868554, a Potent Nonnucleoside Inhibitor of the Hepatitis C Virus RNA-Dependent RNA Polymerase
'PF-00868554 is a nonnucleoside inhibitor of the hepatitis C virus (HCV) RNA polymerase, which exerts its inhibitory effect by binding to the thumb base domain of the protein. It is a potent and selective inhibitor, with a mean 50% inhibitory concentration of 0.019 μM against genotype 1 polymerases and a ... More
Diversity of bacteria associated with grassland soil nematodes of different feeding groups
AuthorsLadygina, N; Johansson, T; Canbaeck, B; Tunlid, A; Hedlund, K
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'The bacterial diversity associated with soil nematodes and its relationship with their feeding habits are as yet poorly understood. In the present study the diversity and abundance of bacteria from nematodes and their surrounding soil were analysed and compared. The nematodes were collected from a grassland soil and sorted into ... More
Quantitative proteomic signature of liver cancer cells: Tissue transglutaminase 2 could be a novel protein candidate of human hepatocellular carcinoma
'Hepatocellular carcinoma (HCC) is one of the most common diseases worldwide, with extremely poor prognosis due to failure in diagnosing it early. Alpha-fetoprotein (AFP) is the only available biomarker for HCC diagnosis; however, its use in the early detection of HCC is limited, especially because about one-third of patients afflicted ... More