ZOOM™ Dual Power Supply (100-120 VAC 47 - 60 Hz) œ - FAQs

以下是更换ZOOM Dual Power Supply保险丝的说明：

注意：ZOOM Dual Power Supply有一个备用保险丝。
1. 关闭 ZOOM Dual Power Supply后方的主电源开关，从 ZOOM Dual Power Supply后方拔掉电源线。
2. 将一字螺丝刀插入开关上方的槽内，转动螺丝刀，轻轻撬开保险丝盒，打开电源接入模块内部的保险丝盒（见使用手册第13页的 ZOOM Dual Power Supply后视图）。
注意：未拔掉电源线时，保险丝盒不会打开。
3. 从盒内拉出保险丝座，并检查保险丝。如果保险丝烧断或保险丝组件有损坏，则使用保险丝座内提供的指定型号保险丝（4 A/250 V）进行更换（见使用手册第39页的图）。
4. 将保险丝座放回盒内。
5. 将盖子扣紧。

这表示电源过热。关闭电源并检查电源风扇周围的气流是否充足。降温后，通过接通电源开关重启电源。如果您无法重启仪器，请关闭电源并从电源插座上拔掉电源线，然后发送邮件至techsupport@lifetech.com来联系技术服务。

这表示在运行期间检测到接地泄漏。应检查电泳系统是否存在不正确的接地情况。然后关闭和打开电源开关以重启电源。

这表示电路中断。请进行以下检查：

- 确认使用了正确的电泳缓冲液。
- 确认所有电线正确连接。
- 关闭电源并重新启动；重新开启应用。
- 如果您无法重启仪器，请关闭电源并从电源插座上拔掉电源线，然后发送邮件至techsupport@lifetech.com来联系技术服务。

以下是可能原因和解决方案：

- 在很低的电流下运行高电压应用。关闭电泳屏幕上的空载警报（详情见使用手册），例如在运行等电点聚焦应用的情况下。
- 电泳导线未连接到电源或电泳装置，或电泳槽出现短路。检查电源和电泳槽的连接处，确保连接完好无损；检查电泳装置中的电线。重新连接好电线以接通电路。按开始/暂停按钮，重新开始电泳。
- 由预制胶上的胶带残留、错误的缓冲液浓度或电泳槽中错误的缓冲液体积导致的高电阻。应确保移除预制胶上的胶带、正确配制缓冲液并在电泳装置中加入推荐的缓冲液体积。

以下是可能原因和解决方案：

- 交流电电源线未连接。检查交流电电源线两端的连接情况。使用正确的电源线。
- 保险丝熔断。按照使用手册中的说明来更换保险丝。

ZOOM Dual Power Supply不需要定期维护，只需偶尔用干布擦拭仪器即可。

我们建议所有需要250V或更低电压的应用都使用ZOOM Dual Power Supply的低电压/高电流侧。

ZOOM Dual Power Supply包含以下组件：ZOOM Dual Power Supply、备用保险丝和电源线。

XCell4 SureLock Midi-Cell应与电泳专用的外加直流电源一起使用。该电源必须：
•与地面绝缘，使直流输出悬空（安全要求），并且必须配备空载或开路保护
•可在200V下运行

如果您想使用XCell4 SureLock Midi-Cell同时进行4块凝胶电泳，我们推荐使用ZOOM Dual Power Supply。其低电压/高电流输出口可处理4块NuPAGE Bis-Tris凝胶的电压和电流（电压200 V，电流约800 mA）。

PowerEase 90W电源和PowerEase 500电源（2013年12月31日起停产）可用于XCell4 SureLock Midi-Cell，但同时进行2两块凝胶电泳（200V 时，达到2 x ~200 mA最大电流）时将达到电流上限500 mA。若使用这些电源在XCell4 SureLock Midi-Cell中同时进行4块凝胶电泳，电流将超出上限，使电泳速度变慢。电泳耗时大约为原来的两倍。

注意：为了将XCell4 SureLock Midi-Cell的隐蔽式电源线连接到不能与隐蔽式或不可伸缩式电源线一起使用的指定电源，请单独使用Invitrogen电源适配器（货号ZA10001）。

The NativePAGE Invitrogen Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analyzing native membrane protein complexes, native soluble proteins, molecular mass estimations, and assessing purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis technique (BN PAGE) developed by Schagger and von Jagow.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

We recommend rinsing the membrane briefly in water, air drying and store it at room temperature in a ziplock bag. Do not place nitrocellulose in the freezer because it will shatter. Unlike PVDF, nitrocellulose membranes should never be pre-wetted in alcohol as it will cause them to shrivel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Here are instructions for replacing the fuse on the ZOOM Dual Power Supply:

Note: One extra fuse is supplied with the ZOOM Dual Power Supply.
- Turn off the main power switch at the rear of the ZOOM Dual Power Supply and detach the power cord from the rear of the ZOOM Dual Power Supply.
- Open the fuse compartment located inside the Power Entry Module (see page xiii of the manual for rear view of the ZOOM Dual Power Supply) by inserting a small flat blade screwdriver into the slot above the On/Off switch. Turn the screwdriver to gently pry open the fuse compartment. Note: The fuse compartment will not open with the power cord in place.
- Pull the fuse holder out of the compartment and inspect the fuse. If the fuse is burned or if there is a break in the fuse element, replace the fuse with the identical type fuse (4 A/250 V) provided in the fuse holder (see figure on page 39 of the manual).
- Place the fuse holder back into the compartment.
- Snap the cover closed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

This indicates that the power supply is overheating. Turn off the power supply and check for sufficient airflow around the power supply fan. After cooling down, restart the power supply by turning the Power switch to the on position. If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet, and contact Technical Support at techsupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

This indicates that a ground leak was detected during the run. Check the electrophoresis system for improper grounding. Restart the power supply by turning the Power switch off and on.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

This indicates that the circuit is interrupted. Please check the following:

- Verify that the running buffer is correct.
- Verify that all cables are attached correctly.
- Turn the Power switch off and on again; restart the application.
- If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet and contact Technical Support at techsupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Here are possible causes and solutions:

- High voltage application is set to run on a very low current. DISABLE No Load alarm on the Running Screen (see manual for details), for example when performing an isoelectric focusing application.
- Electrophoresis leads are not connected to the power supply or to the electrophoresis unit(s), or there is a broken circuit in the electrophoresis cell. Check the connections to the power supply and on your electrophoresis cell to make sure the connection is intact; check condition of wires in electrophoresis unit. Close the circuit by reconnecting the cables. Press Stop/Start to restart the run.
- High resistance due to tape left on a pre-cast gel, incorrect buffer concentration, or incorrect buffer volumes in the electrophoresis cell. Correct the condition by making sure the tape is removed from the pre-cast gel, buffers are prepared correctly, and the recommended volume of buffer is sadded to the electrophoresis unit.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Here are possible causes and solutions:

- AC power cord is not connected. Check AC power cord connections at both ends. Use the correct cords.
- The fuse has blown. Replace the fuse as described in the manual.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The ZOOM Dual Power supply requires no periodic maintenance program with the exception of an occasional dry wipe-down of the instrument.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

We recommend running all applications that require 250 Volts or less on the low voltage/high current side of the ZOOM Dual Power Supply.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The ZOOM Dual Power Supply includes the following components: ZOOM Dual Power Supply, extra fuse, and power cord.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The XCell4 SureLock Midi-Cell should be used with an external DC power supply designed for electrophoresis. This power supply must:

- Be isolated from the ground so that the DC output is floating (safety requirement)and must be equipped with No Load or Open Circuit protection
- Be able to operate at 200 V

If you would like to simultaneously run 4 gels using the XCell4 SureLock Midi-Cell, we recommend using the ZOOM Dual Power Supply. The Low Voltage/High Current outlets can handle the voltage and current of 4 NuPAGE Bis-Tris gels (Voltage 200 V, Current ~800 mA). The PowerEase 90W Power Supply and PowerEase 500 Power Supply (discontinued as of December 31, 2013) can be used with the XCell4 SureLock Midi-Cell but the current limit of 500 mA will be reached with 2 gels (2 x ~200 mA max current at 200V). If 4 gels are run in the XCell4 SureLock Midi-Cell with these power supplies, the current will be maxed out and the run will slow down. It will take approximately twice as long to run the gels. Note: To allow connection of the covered power leads of the XCell4 SureLock Midi-Cell with certain power supplies that are not designed for use with covered or non-retractable power leads, use Invitrogen Power Supply Adapters available separately (Cat. No. ZA10001).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.