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View additional product information for ZOOM™ Dual Power Supply (100-120 VAC 47 - 60 Hz) œ - FAQs (ZP10001)
12 product FAQs found
The NativePAGE Invitrogen Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analyzing native membrane protein complexes, native soluble proteins, molecular mass estimations, and assessing purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis technique (BN PAGE) developed by Schagger and von Jagow.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
We recommend rinsing the membrane briefly in water, air drying and store it at room temperature in a ziplock bag. Do not place nitrocellulose in the freezer because it will shatter. Unlike PVDF, nitrocellulose membranes should never be pre-wetted in alcohol as it will cause them to shrivel.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are instructions for replacing the fuse on the ZOOM Dual Power Supply:
Note: One extra fuse is supplied with the ZOOM Dual Power Supply.
- Turn off the main power switch at the rear of the ZOOM Dual Power Supply and detach the power cord from the rear of the ZOOM Dual Power Supply.
- Open the fuse compartment located inside the Power Entry Module (see page xiii of the manual for rear view of the ZOOM Dual Power Supply) by inserting a small flat blade screwdriver into the slot above the On/Off switch. Turn the screwdriver to gently pry open the fuse compartment. Note: The fuse compartment will not open with the power cord in place.
- Pull the fuse holder out of the compartment and inspect the fuse. If the fuse is burned or if there is a break in the fuse element, replace the fuse with the identical type fuse (4 A/250 V) provided in the fuse holder (see figure on page 39 of the manual).
- Place the fuse holder back into the compartment.
- Snap the cover closed.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
This indicates that the power supply is overheating. Turn off the power supply and check for sufficient airflow around the power supply fan. After cooling down, restart the power supply by turning the Power switch to the on position. If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet, and contact Technical Support at techsupport@thermofisher.com.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
This indicates that a ground leak was detected during the run. Check the electrophoresis system for improper grounding. Restart the power supply by turning the Power switch off and on.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
This indicates that the circuit is interrupted. Please check the following:
- Verify that the running buffer is correct.
- Verify that all cables are attached correctly.
- Turn the Power switch off and on again; restart the application.
- If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet and contact Technical Support at techsupport@thermofisher.com.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- High voltage application is set to run on a very low current. DISABLE No Load alarm on the Running Screen (see manual for details), for example when performing an isoelectric focusing application.
- Electrophoresis leads are not connected to the power supply or to the electrophoresis unit(s), or there is a broken circuit in the electrophoresis cell. Check the connections to the power supply and on your electrophoresis cell to make sure the connection is intact; check condition of wires in electrophoresis unit. Close the circuit by reconnecting the cables. Press Stop/Start to restart the run.
- High resistance due to tape left on a pre-cast gel, incorrect buffer concentration, or incorrect buffer volumes in the electrophoresis cell. Correct the condition by making sure the tape is removed from the pre-cast gel, buffers are prepared correctly, and the recommended volume of buffer is sadded to the electrophoresis unit.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- AC power cord is not connected. Check AC power cord connections at both ends. Use the correct cords.
- The fuse has blown. Replace the fuse as described in the manual.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The ZOOM Dual Power supply requires no periodic maintenance program with the exception of an occasional dry wipe-down of the instrument.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
We recommend running all applications that require 250 Volts or less on the low voltage/high current side of the ZOOM Dual Power Supply.
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The ZOOM Dual Power Supply includes the following components: ZOOM Dual Power Supply, extra fuse, and power cord.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The XCell4 SureLock Midi-Cell should be used with an external DC power supply designed for electrophoresis. This power supply must:
- Be isolated from the ground so that the DC output is floating (safety requirement)and must be equipped with No Load or Open Circuit protection
- Be able to operate at 200 V
If you would like to simultaneously run 4 gels using the XCell4 SureLock Midi-Cell, we recommend using the ZOOM Dual Power Supply. The Low Voltage/High Current outlets can handle the voltage and current of 4 NuPAGE Bis-Tris gels (Voltage 200 V, Current ~800 mA). The PowerEase 90W Power Supply and PowerEase 500 Power Supply (discontinued as of December 31, 2013) can be used with the XCell4 SureLock Midi-Cell but the current limit of 500 mA will be reached with 2 gels (2 x ~200 mA max current at 200V). If 4 gels are run in the XCell4 SureLock Midi-Cell with these power supplies, the current will be maxed out and the run will slow down. It will take approximately twice as long to run the gels. Note: To allow connection of the covered power leads of the XCell4 SureLock Midi-Cell with certain power supplies that are not designed for use with covered or non-retractable power leads, use Invitrogen Power Supply Adapters available separately (Cat. No. ZA10001).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.