50 bp DNA 分子量标准
50 bp DNA 分子量标准
Invitrogen™

50 bp DNA 分子量标准

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Invitrogen 50 bp DNA 分子量标准设计用于 50 bp 至 2,500 bp了解更多信息
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货号数量
1041601450 μg
货号 10416014
价格(CNY)
658.00
Online Exclusive
Ends: 31-Dec-2026
2,617.00
共减 1,959.00 (75%)
Each
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数量:
50 μg
请求批量或定制报价
价格(CNY)
658.00
Online Exclusive
Ends: 31-Dec-2026
2,617.00
共减 1,959.00 (75%)
Each
添加至购物车
Invitrogen 50 bp DNA 分子量标准设计用于 50 bp 至 2,500 bp 内双链 DNA 片段的大小鉴定和近似定量。50 bp DNA 分子量标准由 17 个层析纯化的个体 DNA 片段组成,参比条带分别为 2500、800 和 350 bp,便于轻松定向。

50 bp DNA 分子量标准非常适合利用 2% 琼脂糖凝胶进行 DNA 片段分离。

50 bp DNA 分子量标准的特点:
明显、清晰的条带—层析纯化片段可获得一致和可靠的结果
便利—已经预混了 6X TrackIt Cyan/Orange 上样缓冲液,便于追踪样品 DNA 迁移
精确—可获得每条带中的 DNA 精确量

产品用途
溴化乙锭或 SYBR Safe 染色后,可在 2% 琼脂糖凝胶上观测到双链 DNA 分子量标准。该分子量标准设计有强度均匀的 DNA 条带,便于清楚查看每一条带。每个条带中确切量的 DNA 可实现样品 DNA 近似定量。

该分子量标准可使用 T4 多聚核苷酸激酶或 T4 DNA 聚合酶进行放射性标记。

仅供科研使用。不可用于诊断程序。
规格
最大浓度0.5 μg/μL
凝胶兼容性琼脂糖凝胶
环保功能绿色可持续包装
反应次数100应用
产品类型DNA 分子量标准品
数量50 μg
即用型
上样容量1 mL
运输条件经批准可在室温或干冰上运输
技术层析纯化的个体 DNA 片段
容积(公制)100 µL
凝胶类型琼脂糖
大小范围50 bp - 2,500 bp
Unit SizeEach
内容与储存
• 100 µL 50 bp DNA 分子量标准
• 1 mL 6X TrackIt Cyan/Orange 上样缓冲液

储存于 -20°C。

常见问题解答 (FAQ)

我的DNA ladder出现异常迁移。这是为什么?

如果marker被加热了,会出现这种情况。请确保DNA ladder在使用前没有被加热过。

TrackIt DNA梯度标准品与其他DNA梯度标准品有何区别?

TrackIt DNA梯度标准品的样品缓冲液中含有2种示踪染料,可作为观察凝胶电泳进程的视觉指示分子,并能指示何时达到最大分辨率。示踪染料不会遮盖梯度标准品中的DNA条带,因为染料会跑出梯度标准品中大部分DNA条带的范围。
TrackIt蓝绿色/橙黄色上样缓冲液的配方含有独特的示踪染料,二甲苯蓝FF和橙黄G。我们推荐将TrackIt蓝绿色/橙黄色上样缓冲液用于10bp至1kb间的DNA片段。
TrackIt蓝绿色/黄色上样缓冲液含有独特的示踪染料,二甲苯蓝FF和柠檬黄。我们推荐将TrackIt蓝绿色/黄色上样缓冲液用于100bp至10kb间的DNA片段。二甲苯蓝FF的分子量为638.6,橙黄G为452.4,柠檬黄为534.4。
备注:不推荐将TrackIt DNA 梯度标准品用于聚丙烯酰氨凝胶,并且不推荐用于定量分析。

Can I know the sequences of Invitrogen DNA ladders?

Sequences of Invitrogen DNA and RNA ladders are proprietary.

Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?

Invitrogen DNA ladders contain linear dsDNA fragments.

Are Invitrogen DNA ladders composed of single-stranded or double-stranded DNA fragments?

Invitrogen DNA ladders are composed of double-stranded DNA fragments only.

引用和文献 (10)

引用和文献
Abstract
ChIP-Seq using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites.
Authors:Lefrançois P, Zheng W, Snyder M
Journal:Methods Enzymol
PubMed ID:20946807
'Much of eukaryotic gene regulation is mediated by binding of transcription factors near or within their target genes. Transcription factor binding sites (TFBS) are often identified globally using chromatin immunoprecipitation (ChIP) in which specific protein-DNA interactions are isolated using an antibody against the factor of interest. Coupling ChIP with high-throughput ... More
Post intrastromal corneal ring segments insertion complicated by Candida parapsilosis keratitis.
Authors:Mitchell BM, Kanellopoulos AJ, Font RL
Journal:Clin Ophthalmol
PubMed ID:23467516
'This case report describes the clinical and histopathologic features, including molecular confirmation, of fungal keratitis after intrastromal corneal ring segments placement for keratoconus. A 52-year-old woman underwent insertion of Intacs(®) corneal implants for treatment of keratoconus. Extrusion of the implants was noted 5 months post insertion and replaced. Three months ... More
Development of PCR-RFLP assay for the discrimination of Plasmodium species and variants of P. vivax (VK210, VK247 and P. vivax-like) in Anopheles mosquitoes.
Authors:Cassiano GC, Storti-Melo LM, Póvoa MM, Galardo AK, Rossit AR, Machado RL
Journal:Acta Trop
PubMed ID:21420375
'The identification of Plasmodium species in Anopheles mosquitoes is an integral component of malaria control programs. We developed a new assay to identify Plasmodium falciparum, Plasmodium malariae, and Plasmodium vivax variants. Specific primers were designed to hybridize to CS gene-specific regions. Polymerase chain reaction (PCR) and restriction fragment length polymorphism ... More
Report of an international collaborative study to evaluate the suitability of multiplex PCR as an identity assay for different sub-strains of BCG vaccine.
Authors:Markey K, Ho MM, Choudhury B, Seki M, Ju L, Castello-Branco LR, Gairola S, Zhao A, Shibayama K, Andre M, Corbel MJ
Journal:Vaccine
PubMed ID:20732463
'Current methods for the identification of BCG vaccine in quality control settings involve acid-fast staining with microscopic examination. However, this method is unable to distinguish the many different sub-strains of BCG, or to differentiate BCG strains from virulent members of the Mycobacterium tuberculosis complex. A multiplex PCR (mPCR) which uses ... More
Identification of novel mutation in cathepsin C gene causing Papillon-Lefèvre Syndrome in Mexican patients.
Authors:Romero-Quintana JG, Frías-Castro LO, Arámbula-Meraz E, Aguilar-Medina M, Dueñas-Arias JE, Melchor-Soto JD, Romero-Navarro JG, Ramos-Payán R
Journal:BMC Med Genet
PubMed ID:23311634
Papillon-Lefèvre Syndrome (PLS) is a type IV genodermatosis caused by mutations in cathepsin C (CTSC), with a worldwide prevalence of 1-4 cases per million in the general population. In México, the prevalence of this syndrome is unknown, and there are few case reports. The diagnosis of twenty patients in the ... More